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Identification of different ABA biosynthesis sites at seedling and fruiting stages in Arachis hypogaea L. following water stress

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Abstract

Abscisic acid (ABA) is one of the most important phytohormones involved in abiotic stress responses. ABA transport in plants is important in determining endogenous ABA levels and their resulting physiological responses. However, the regulation of ABA transport remains unclear. In this study, we compared the ABA concentrations and AhNCED1 levels at seedling and fruiting stages in peanut (Arachis hypogaea L.), in response to water stress. At the seedling stage, ABA initially accumulated in roots (1 h), followed by the lower stem (2 h) and finally in the upper stem (4 h). The expression/activity of an ABA biosynthesis rate-limiting enzyme, AhNCED1, showed the same accumulation patterns. In contrast, during the fruiting stage, ABA and AhNCED1 increases were initially detected in the first apical leaf of main stem, followed by the stem, and finally in the root. These results imply that biosynthesis of ABA in peanut plants subject to water deficiency could be dependent on developmental stage with the roots being the initial site of ABA biosynthesis during the seedling stage, whereas during the fruiting stage ABA biosynthesis occurs initially in the leaf. The distribution patterns of ABA in seedling stage peanuts in response to water stress were: root-stem-leaf, while in fruiting stage peanuts the distribution patterns of ABA were: leaf-stem-root. These findings will help to understand plant regulatory water deficit resistance mechanisms at seedling and fruiting stages and to advance our total understanding of the regulation of ABA transport.

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Acknowledgments

We are very grateful to Dr. Nicole Grant at University of Technology, Sydney for critical reading of the manuscript. This work was supported by Natural Science Foundation of China (30971715 to Ling Li), Guangdong Natural Science Foundation (915106310100001 and 10251063101000010 to Ling Li), China Postdoctoral Science Foundation (20110490907 to Dr. Bo Hu) and Science and Technology Planning Project of Guangdong Province (2011B020301009 to Dr. Bo Hu).

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Authors and Affiliations

  1. Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, 510631, People’s Republic of China

    B. Hu, L. Hong, X. Liu, S. N. Xiao, Y. Lv & L. Li

  2. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, People’s Republic of China

    L. Hong

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  1. B. Hu
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  2. L. Hong
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  3. X. Liu
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  4. S. N. Xiao
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  5. Y. Lv
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  6. L. Li
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Correspondence to L. Li.

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Bo Hu and Lan Hong contributed equally to this work.

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Hu, B., Hong, L., Liu, X. et al. Identification of different ABA biosynthesis sites at seedling and fruiting stages in Arachis hypogaea L. following water stress. Plant Growth Regul 70, 131–140 (2013). https://doi.org/10.1007/s10725-013-9785-8

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  • DOI: https://doi.org/10.1007/s10725-013-9785-8

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