Abstract
Protocols for in vitro propagation of non-toxic variety of J. curcas through axillary bud proliferation and direct adventitious shoot bud regeneration from leaf segments have been established. Shoot bud proliferation from axillaries was assessed on an initial basal Murashige and Skoog (MS) salt medium supplemented with different concentrations of benzyladenine (BA), kinetin and thidiazuron (TDZ) followed by subculture to medium with 4.4-8.9 μM BA. Regardless of the concentration of BA in the subculture medium, shoot multiplication rate was optimum (10–12.3) with primary culture on medium supplemented with 2.3–4.5 μM TDZ. Efficient adventitious shoot regeneration from leaf tissues was achieved with culture on medium with 8.9–44.4 μM BA + 4.9 μM indole-3-butyric acid (IBA) followed by transfer to medium supplemented with 8.9 μM BA + 2.5 μM IBA. Similarity index between toxic Indian variety and the non-toxic variety based on 435 RAPD markers was 96.3%. Crossing studies followed by phorbol ester quantitation revealed that outcrosses with toxic J. curcas do not affect the phorbol ester content of seeds borne on the non-toxic variety.
Similar content being viewed by others
Abbreviations
- BA:
-
N6-benzyladenine
- 1BA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog (1962) medium
- RAPD:
-
random amplified polymorphic DNA
- TDZ:
-
1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron)
References
Becker K. and Makkar H.P.S. 1997. Potential of Jatropha seed cake as protein supplement in livestock feed and constraints to its utilization. In Proceedings of Jatropha 97: International Symposium on Biofuel and Industrial Products from Jatropha curcas and Other Tropical Oilseed Plants 23–27 Feb. 1997. Managua/NicaraguaMexico.
K. Becker H.P.S. Makkar (1998) ArticleTitleEffects of phorbol esters in carp (Cyprinus carpio L.) Vet. Hum. Toxicol. 40 82–89 Occurrence Handle9554059
B. Dehgan (1984) ArticleTitlePhylogenetic significance of interspecific hybridization in Jatropha (Euphorbiaceae) Syst. Bot. 9 467–478
S.J. Dellaporta J. Wood J.B. Hicks (1983) ArticleTitleA plant DNA minipreparation. Version II Plant Mol. Biol. Rep. 1 19–21
George E.F. 1993. Plant propagation by tissue culture Part I. The technology. Exegetics Ltd.
G.M. Gubitz M. Mittelbach M. Trabi (1999) ArticleTitleExploitation of the tropical oil seed plant Jatropha curcas L Bioresource Technol. 67 73–82 Occurrence Handle10.1016/S0960-8524(99)00069-3
Heller J. 1996. Physic nut – Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. 1. International Plant Genetic Resources InstituteRomeItaly (http://www.ipgri.cgiar.org/publications/pdf/161.pdf).
C.A. Huetteman J.E. Preece (1993) ArticleTitleThidiazuron: a potent cytokinin for woody plant tissue culture Plant Cell Tiss. Org. Cult. 33 105–119 Occurrence Handle10.1007/BF01983223
M. Jayasingh (2004) The use of biodiesel by the Indian railways N.G. Hegde J.N. Daniel S. Dhar (Eds) Jatropha and Other Perennial Oilseed Crops BAIF Development Research Foundation PuneIndia 31–33
N. Jones J.H. Miller (1991) ArticleTitleJatropha curcas. A multipurpose species for problematic sites Land Resour. Ser. 1 1–12
C.W. Lee J. Yeckes J.C. Thomas (1982) ArticleTitleTissue culture propagation of Euphorbia lathyris Asclepias erosa HortScience 17 533
H.P.S. Makkar A.O. Aderibigbe K. Becker (1998) ArticleTitleComparative evaluation of non-toxic and toxic varieties of Jatropha curcas for chemical composition, digestibility, protein degradability and toxic factors Food Chem. 62 207–215 Occurrence Handle10.1016/S0308-8146(97)00183-0
T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473–497
N.G. Nair K.K. Kartha O.L. Gamborg (1979) ArticleTitleEffect of growth regulators on plant regeneration from shoot apical meristems of cassava (Manihot esculenta Crantz) and on the culture of internodes in vitro Z. Pflanzenphysiol. 95 51–56
K. Openshaw (2000) ArticleTitleA review of Jatropha curcas: an oil plant of unfulfilled promise Biomass Bioenerg. 19 1–15 Occurrence Handle10.1016/S0961-9534(00)00019-2
A.J. Prabakaran M. Sujatha (1999) ArticleTitle Jatropha tanjorensis Ellis & Sarojaa natural interspecific hybrid occurring in Tamil NaduIndia Genet. Resour. Crop Evol. 46 213–218 Occurrence Handle10.1023/A:1008635821757
K.P. Ripley J.E. Preece (1986) ArticleTitleMicropropagation of Euphorbia lathyrus L Plant Cell Tiss. Org. Cult. 5 213–218 Occurrence Handle10.1007/BF00040132
M. Sujatha M. Dhingra (1993) ArticleTitleRapid plant regeneration from various explants of Jatropha integerrima Plant Cell Tiss. Org. Cult. 35 293–296 Occurrence Handle10.1007/BF00037284
M. Sujatha N. Mukta (1996) ArticleTitleMorphogenesis and plant regeneration from tissue cultures of Jatropha curcas Plant Cell Tiss. Org. Cult. 44 135–141 Occurrence Handle10.1007/BF00048191
M. Sujatha A.J. Prabakaran (2003) ArticleTitleNew ornamental Jatropha hybrids through interspecific hybridization Genet. Resour. Crop Evol. 50 75–82 Occurrence Handle10.1023/A:1022961028064
M. Sujatha T.P. Reddy (1998) ArticleTitleDifferential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L) Plant Cell Rep. 17 561–566 Occurrence Handle10.1007/s002990050442
M. Sujatha T.P. Reddy (2000) ArticleTitleMorphogenic responses of Jatropha integerrina explants to cytokinins Biologia 55 99–104
J. Tideman J.S. Hawker (1982) ArticleTitle In vitro propagation of latex producing plants Ann. Bot. 49 273–279
J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res. 18 6531–6535 Occurrence Handle1979162
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sujatha, M., Makkar, H. & Becker, K. Shoot Bud Proliferation from Axillary Nodes and Leaf Sections of Non-toxic Jatropha curcas L.. Plant Growth Regul 47, 83–90 (2005). https://doi.org/10.1007/s10725-005-0859-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10725-005-0859-0