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Development of DArT markers and evaluation of phylogenetic relationship of key Citrus species

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Abstract

In the past decades, studies based on DNA markers have been widely conducted to understand the phylogenetic relationship of Citrus species. The recent development of diversity arrays technology (DArT) offers new possibilities to study the phylogenetic relationship of Citrus species. Here, we report the successful development of DArT microarrays and their utility in phylogenetic analysis of Citrus species. The DArT microarrays revealed a total 727 polymorphic markers with an average call rate of 91.16 % and a scoring reproducibility of 96.5 %. Phylogenetic analyses based on DArT markers strongly supported delineation of the genus Citrus as a monophyletic group. Most Citrus species analyzed formed different clusters, but Ichang papeda and Mangshan wild mandarin formed separate clusters as two single branches, suggesting that various mandarin accessions have different genetic backgrounds. As the first attempt to develop a high-throughput DArT markers system for the genus Citrus, the present study generated significant information about the genetic relationship between cultivated species and their putative wild progenitors, which suggested that the DArT markers system could be a powerful tool to study the genetic relationships of the genus Citrus.

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Acknowledgments

The research was supported by National Natural Science Foundation of China (31171930).

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Authors and Affiliations

  1. College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400715, China

    Xiaogang Liu, Liang Tang, Hongkun Wu, Wanpeng Xi, Jie Yu & Zhiqin Zhou

  2. Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing, 400715, China

    Xiaogang Liu, Liang Tang, Hongkun Wu, Wanpeng Xi, Jie Yu & Zhiqin Zhou

  3. Post-doctoral Station of Horticulture, Southwest University, Chongqing, 400715, China

    Xiaogang Liu

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  1. Xiaogang Liu
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Liu, X., Tang, L., Wu, H. et al. Development of DArT markers and evaluation of phylogenetic relationship of key Citrus species. Genet Resour Crop Evol 63, 1307–1318 (2016). https://doi.org/10.1007/s10722-015-0319-2

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