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Molecular and immune response characterizations of a novel Bax inhibitor-1 gene in pufferfish, Takifugu obscurus

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Abstract

Apoptosis plays a crucial role in many biological processes, including development, cellular homeostasis, and immune responses. Bax inhibitor-1 (BI-1) is an anti-apoptotic protein that protects cells from endoplasmic reticulum stress-induced apoptosis. In this study, a BI-1 gene from the pufferfish Takifugu obscurus (Pf-BI-1) was identified and characterized. The full length of Pf-BI-1 cDNA was 1387 bp, including a 5′-UTR of 82 bp, a 3′-UTR of 591 bp containing a poly-(A) tail, and an open reading frame (ORF) of 714 bp that encodes a polypeptide of 237 amino acids. Pf-BI-1 was ubiquitously expressed in various tissues, with the highest expression levels in the blood, brain, and gill. The expression of Pf-BI-1 was up-regulated in a time-dependent manner after heat shock stress, ammonia stress, and bacterial challenge. Intracellular localization revealed that Pf-BI-1 was primarily localized in the cell cytoplasm. Furthermore, over-expression of Pf-BI-1 could active NF-кB reporter genes in HeLa cells. These results indicated that Pf-BI-1 may be involved in the apoptosis and immunity process against ambient stressors in pufferfish.

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Acknowledgements

This research was supported by Hainan Applied Technology Research and Development and Demonstration Projects (ZDXM2015025), the China Postdoctoral Science Foundation (2012M511829), the Science and Technology Innovation of Higher School Guangdong Province (cxzd114). Special Funds for Fish Diseases of Guangdong province (2015-352 and 2016-302), Marine Fisheries Technology and Industry Development Special Projects of Guangdong Province (A201501B14), Marine Fisheries Science and Technology Extension Projectsof Guangdong Province (B201601-11), Science and technology Projects of Guangdong Province (2014B040404057).

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Authors and Affiliations

  1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510300, China

    Chang-Hong Cheng & Zhi-Xun Guo

  2. Key Laboratory of Ecology and Environmental Science of Guangdong, Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, People’s Republic of China

    Sheng-Wei Luo & An-Li Wang

  3. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center (SCS-REPIC), Guangzhou, People’s Republic of China

    Zhi-Xun Guo

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  1. Chang-Hong Cheng
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  2. Sheng-Wei Luo
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  3. An-Li Wang
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  4. Zhi-Xun Guo
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Correspondence to An-Li Wang or Zhi-Xun Guo.

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Cheng, CH., Luo, SW., Wang, AL. et al. Molecular and immune response characterizations of a novel Bax inhibitor-1 gene in pufferfish, Takifugu obscurus . Fish Physiol Biochem 43, 965–975 (2017). https://doi.org/10.1007/s10695-016-0337-9

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