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Probiotic bacteria Lactobacillus rhamnosus influences the blood profile in rainbow trout Oncorhynchus mykiss (Walbaum)

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Abstract

This paper reports the effect of feeding probiotic diets on blood profiles in rainbow trout. Two experiments were performed: in the first, fish of average weight 75 g were offered either a commercial feed or the same incorporated with 109 CFU g−1 of lactic acid bacteria Lactobacillus rhamnosus for 30 days; in the second study performed for a similar duration, fish of average weight 126 g were offered formulated diets that either contained the same bacteria in heat-killed or freeze-dried form (nearly 1011 CFU g−1), or the basal diet without the bacteria. Blood samples were collected at different times after commencement of probiotic feeding to determine the total cholesterol, triglyceride contents, the plasma alkaline phosphatase activity, plasma protein and hematocrit value. The plasma cholesterol significantly increased upon probiotic feeding in the first experiment. A significant elevation (P < 0.05) of plasma cholesterol and triglyceride and alkaline phosphatase activity level was found in the freeze-dried probiotic fed groups at 20 and 30 days postfeeding. This was concomitant with the increased plasma protein and hematocrit values in FD group at 20 and 30 days. Likewise, the heat-killed probiotic fed group registered significantly high values of triglycerides, alkaline phosphatase activity, and plasma protein compared to the control diet fed groups after 20 days of feeding. Thus, alterations in the blood profiles could serve as supplementary information when examining the benefits of probiotics for fish.

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References

  • Bengmark S (2001) Nutritional modulation of acute and chronic phase responses. Nutrition 17:489–495

    Article  CAS  PubMed  Google Scholar 

  • Boyd RD, Hall D, Wu JF (1983) Plasma alkaline phosphatase as a criterion for determining biological availability of phosphorus for swine. J Anim Sci 57:396–401

    CAS  PubMed  Google Scholar 

  • Canzi E, Zanchi R, Camaschella P, Cresci A, Greppi GF, Orpianesi C et al (2000) Modulation by lactic acid bacteria of the intestinal ecosystem and plasma cholesterol in rabbits fed a casein diet. Nutr Res 20:1329–1340

    Article  CAS  Google Scholar 

  • Chatzifotis S, Muje P, Pavlidis M, Agren J, Paalavuo M, Molsa H (2004) Evolution of tissue composition and serum metabolites during gonadal development in the common dentex (Dentex dentex). Aquaculture 236:557–573

    Article  CAS  Google Scholar 

  • De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135

    Google Scholar 

  • De Rodas BZ, Gilliland SE, Maxwell CV (1996) Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J Dairy Sci 79:2121–2128

    Article  PubMed  Google Scholar 

  • De Roos NM, Katan MB (2000) Effect of probiotic bacteria on diarrhea, lipid metabolism, carcinogenesis. A review of papers published between 1988 to 1998. Am J Clin Nutr 71:405–411

    PubMed  Google Scholar 

  • Fishbein L, Kaplan M, Gough M (1998) Fructooligosaccharides: a review. Vet Hum Toxicol 30(2):104–107

    Google Scholar 

  • Fukuda Y, Maita M, Satoh K, Okamoto N (1997) Influence of dissolved oxygen concentration during pre and post infection on the mortality induced by artificial infection with Enterococcus seriolicida in yellow tail. Fish Pathol 32:129–130

    Google Scholar 

  • Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66:365–378

    CAS  PubMed  Google Scholar 

  • Fuller R (1992) Probiotics: the scientific basis. Chapman & Hall, London

    Google Scholar 

  • Gerwick L, Steinhauer R, Lapatra S, Sandell T, Ortuno J, Hajiseyedjavadi N et al (2002) The acute phase response of rainbow trout (Oncorhynchus mykiss) plasma proteins to viral, bacterial and fungal inflammatory agents. Fish Shellfish Immunol 12:229–242

    Article  CAS  PubMed  Google Scholar 

  • Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

    CAS  PubMed  Google Scholar 

  • Gill HS (1998) Stimulation of the immune system by lactic culture. Int Dairy J 8:535–544

    Article  CAS  Google Scholar 

  • Gorbach SL (1990) Lactic acid bacteria and human health. Ann Med 22:7–41

    Article  Google Scholar 

  • Harikrishnan R, NishaRani M, Balasundaram C (2003) Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture 221:41–50

    Article  Google Scholar 

  • Hille S (1982) A literature review of the blood chemistry of rainbow trout Salmo gairdneri Rich. J Fish Biol 20:535–569

    Article  CAS  Google Scholar 

  • Jahreis G, Vogelsang H, Kiessling G, Schubert R, Hammes WP (2002) Influence of probiotic sausage (Lactobacillus paracasei) on blood lipids and immunological parameters of healthy volunteers. Food Res Int 35:133–138

    Article  CAS  Google Scholar 

  • Jeney G, Galeotti M, Volpatti D, Jeney Z, Douglas PA (1997) Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture 154:1–15

    Article  CAS  Google Scholar 

  • Jonsson E, Conway P (1992) Probiotics for pigs. In: Fuller R (ed) Probiotics. The scientific basis. Chapman and Hall, London, pp 259–316

    Google Scholar 

  • MacDonal DG, Milligan CL (1992) Chemical properties of blood. In: Hoar WS, Randall DJ, Farrell AP (eds) Fish physiology. Academic Press, San Diego, pp 56–133

    Google Scholar 

  • Maita M (2003) Plasma cholesterol as an index for evaluating health of farmed fish. World Aquaculture Society Book of Abstracts, Salvador, p 444

    Google Scholar 

  • Maita M, Ushio H, Okamoto N (1996) The effect of anemia artificially induced by bleeding on blood constituents, concentration of ATP-related compounds and Na+-K+ ATPase activity in gill and kidney of coho salmon. Fish Pathol 31:237–238

    CAS  Google Scholar 

  • Maita M, Aoki H, Yamagata Y, Satoh S, Okamoto M, Watanabe T (1998a) Plasma biochemistry and disease resistance in yellowtail fed a non-fish meal diet. Fish Pathol 33:59–63

    CAS  Google Scholar 

  • Maita M, Aoki H, Satoh K, Fukuda Y, Lee H, Winton JR et al (1998b) Correlation between plasma component levels of cultured fish and resistance to bacterial infection. Fish Pathol 33:129–133

    CAS  Google Scholar 

  • Maita M, Satoh K, Satoh S, Kiron V, Watanabe T (2002) Effects of non-fish meal diet on hematological parameters, disease resistance and lipid profiles of liver and erythrocytes in yellow tail. World Aquaculture Society Book of Abstracts, Beijing, p 475

    Google Scholar 

  • Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S (2001) Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture 198:229–236

    Article  Google Scholar 

  • Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius E (2003) Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish Shellfish Immunol 15:443–452

    Article  CAS  PubMed  Google Scholar 

  • Pacini N, Ferrari A, Zanchi R, Corti M, Greppi GF (1989) The effect of protein dietary sources on serum cholesterol and bile acid microbiological transformation in rabbits. Nutr Rep Int 39:851–859

    CAS  Google Scholar 

  • Panigrahi A, Kiron V, Kobayashi T, Puangkaew J, Satoh S, Sugita H (2004) Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Vet Immunol Immunopathol 102:379–388

    Article  CAS  PubMed  Google Scholar 

  • Panigrahi A, Kiron V, Puangkaew J, Kobayashi T, Satoh S, Sugita H (2005) The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquaculture 243:241–254

    Article  Google Scholar 

  • Periera IAD, Gibson RG (2002) Effect of consumption of probiotics and prebiotics on serum lipid levels in humans. Crit Rev Biochem Mol Biol 37:259–281

    Article  Google Scholar 

  • Quan S, Gill HS (2002) Immune protection mediated by the probiotic Lactobacillus rhamnosus HN001 (DR 20) against Escherichia coli 0157:H7 infection in mice. FEMS Immunol Med Microbiol 34:59–64

    Google Scholar 

  • Sindhu SC, Khetarpaul N (2002) Effect of feeding probiotic fermented indigenous food mixture on serum cholesterol levels in mice. Nutr Res 23:1071–1080

    Article  Google Scholar 

  • Standiford TJ, Arenberg DA, Danforth JM, Kunkel SL, VanOtteren GM, Strieter RM (1994) Lipoteichoic acid induces secretion of interleukin-8 from human blood monocyte: a cellular and molecular analysis. Infect Immun 62:119–125

    CAS  PubMed  Google Scholar 

  • Sugiura SH, Hardy RW, Roberts RJ (2004) The pathology of phosphorus deficiency in fish—a review. J Fish Dis 27:255–265

    Article  CAS  PubMed  Google Scholar 

  • Tortuero F, Fernandez E, Ruperez P, Moreno M (1997) Raffinose and lactic acid bacteria influence caecal fermentation and serum cholesterol in rats. Nutr Res 17:41–49

    Article  CAS  Google Scholar 

  • Vershuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in Aquaculture. Microbiol Mol Biol Rev 64:655–671

    Article  Google Scholar 

  • Villamil L, Tafalla C, Figueras A, Novoa B (2002) Evaluation of immunomodulatory effect of lactic acid bacteria in turbot (Scophthalmus maximus). Clin Diagn Lab Immunol 9:1318–1323

    CAS  PubMed  Google Scholar 

  • Watanabe T, Aoki H, Viyakaran V, Maita M, Yamagata Y, Satoh S et al (1995) Combined use of alternative protein sources as a partial replacement for fish meal in a newly developed soft dry pellet for yellowtail. Suisan zoshoku 43:511–520

    Google Scholar 

  • Wedemeyer GA, Mcleay DJ (1981) Methods for determining the tolerance of fishes to environmental stressors. In: Pickering AD (ed) Stress and fish. Academic Press, London, pp 246–275

    Google Scholar 

  • Young J (1998) European market developments in prebiotic- and probiotic-containing foodstuffs. Br J Nutr 80(4):231–233

    Google Scholar 

Download references

Acknowledgments

This work was partly supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and the internal research funds for advanced research from the Tokyo University of Marine Science and Technology. The Fuji Trout Hatchery of Shizuoka Prefecture Fisheries Experimental Station is thanked for providing the fish used for the study.

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Authors and Affiliations

  1. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, Tokyo, 108-8477, Japan

    A. Panigrahi, V. Kiron, S. Satoh & T. Watanabe

  2. Central Institute of Brackish Water Aquaculture, 75, Santhom High Road, R. A. Puram, Chennai, 600-028, India

    A. Panigrahi

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  1. A. Panigrahi
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  2. V. Kiron
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  3. S. Satoh
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  4. T. Watanabe
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Correspondence to A. Panigrahi.

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Panigrahi, A., Kiron, V., Satoh, S. et al. Probiotic bacteria Lactobacillus rhamnosus influences the blood profile in rainbow trout Oncorhynchus mykiss (Walbaum). Fish Physiol Biochem 36, 969–977 (2010). https://doi.org/10.1007/s10695-009-9375-x

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  • DOI: https://doi.org/10.1007/s10695-009-9375-x

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