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Identification of QTLs associated with p-coumaric acid and ferulic acid in barley

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Abstract

Phenolic acids in plants is receiving more attention because of their nutritive function and essential roles in plant growth. However, the genetics of phenolic acids in barley is still unclear. In this study, two main phenolic acids, i.e. ferulic acid (FA) and p-coumaric acid (p-CA), were analyzed in the grains of a double haploid population derived from XZ153 (Tibetan wild barley) × Hua30 (cultivated cultivar) grown in three locations. A wide variation in the content of phenolic acids was observed among the DH population. Quantitative trait locus (QTL) analysis of phenolic acid content in barley grains from three locations totally identified 4 QTLs associated with p-CA content and 4 QTLs with FA content. Based on the mean phenolic acids content over the three locations, two significant QTLs (M_3261686 for p-CA and M_4184755 for FA), belonging to cytochrome P450 family (HvCYPs) were identified, with the LOD value of 5.33 and 7.01 respectively. These two QTLs could account for 14% of phenotypic variation for FA and 18% for p-CA. Collinearity analysis revealed that HvCYPPA (QTL for p-CA) has duplication events in barley genome. Furthermore, there was no synteny block found for these two genes in barley genome. The current results provide a new knowledge about genetic controlling of phenolic acids synthesis in barley grains.

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Acknowledgements

We thank China Agriculture Research System (CARS-05) and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP) for experimental implementation, sampling and data analysis. We also thank Jingqun Yuan, the technician of 985-Institute of Agrobiology and Environmental Sciences of Zhejiang University, for the assistance in HPLC analysis.

Funding

This study was supported by Natural Science Foundation of China (31620103912), China Agriculture Research System (CARS-05) and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).

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Authors and Affiliations

  1. Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    Zhigang Han, Huaizhou Gao, Lingzheng Ye, Muhammad Faheem Adil, Muhammad Ahsan & Guoping Zhang

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  1. Zhigang Han
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  2. Huaizhou Gao
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  3. Lingzheng Ye
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  4. Muhammad Faheem Adil
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  5. Muhammad Ahsan
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  6. Guoping Zhang
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Correspondence to Guoping Zhang.

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Han, Z., Gao, H., Ye, L. et al. Identification of QTLs associated with p-coumaric acid and ferulic acid in barley. Euphytica 215, 198 (2019). https://doi.org/10.1007/s10681-019-2526-y

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