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Molecular mapping of quantitative trait loci for zinc, iron and protein content in the grains of hexaploid wheat

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Abstract

Understanding the genetic basis of micronutrient concentration in wheat grain may provide useful information to breed for biofortified varieties through marker assisted selection (MAS). One hundred and thirty eight doubled haploid progeny of a cross between the wheat cultivars ‘Berkut’ and ‘Krichauff’ were evaluated for 2 years at two locations on the eastern Gangetic plains of India under timely (November) sown conditions. Grains were evaluated for Zn and Fe concentrations by energy-dispersive X-ray fluorescence. Using composite interval mapping, three QTLs were identified; two for Zn (1B and 2B) with a QTL (2B) co-located for Fe and the third (1A), for protein. The QTL located on chromosome 1B (flanked by wmc036–cfa2129) and 2B (flanked by gwm120–wpt2430) for Zn explained up to 23.1 and 35.9 % of mean phenotypic variation respectively, whereas up to 22.2 % was explained by the Fe QTL co-located with the Zn QTL on chromosome 2B. A QTL for grain protein was detected on chromosome 1A and flanked by the markers, wpt9592 and GBM1153 which explained up to 17.7 % of the total phenotypic variation. With their detection over consecutive seasons, the detected QTLs appeared robust and useful for MAS.

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Acknowledgments

The authors are grateful for constructive suggestions made by Wolfgang Pfeifer (HarvestPlus) and Ravi P. Singh and Hans J. Braun (CIMMYT, Mexico) in the course of this study. Part of this work was possible due to facilities provided under HarvestPlus project at Banaras Hindu University. Authors offer sincere thanks to the HarvestPlus for this support.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Chhavi Tiwari, Balasubramaniam Arun, Vinod Kumar Mishra & Arun Kumar Joshi

  2. South Australian Research and Development Institute, GPO Box 397, Adelaide, 5001, Australia

    Hugh Wallwork

  3. International Maize and Wheat Improvement Center (CIMMYT), Apdo., Postal 6-641, C.P. 06600, Mexico, D.F., Mexico

    Govindan Velu & Uttam Kumar

  4. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, 5001, Australia

    James Stangoulis

  5. CIMMYT, South Asia Regional Office, Singh Durbar Marg, Kathmandu, Nepal

    Arun Kumar Joshi

Authors
  1. Chhavi Tiwari
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  2. Hugh Wallwork
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  3. Balasubramaniam Arun
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  4. Vinod Kumar Mishra
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  5. Govindan Velu
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  6. James Stangoulis
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  7. Uttam Kumar
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  8. Arun Kumar Joshi
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Correspondence to Arun Kumar Joshi.

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Tiwari, C., Wallwork, H., Arun, B. et al. Molecular mapping of quantitative trait loci for zinc, iron and protein content in the grains of hexaploid wheat. Euphytica 207, 563–570 (2016). https://doi.org/10.1007/s10681-015-1544-7

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  • DOI: https://doi.org/10.1007/s10681-015-1544-7

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