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Different recombination frequencies in wheat doubled haploid populations obtained through maize pollination and anther culture

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Abstract

This study compared the meiotic recombination frequency between wheat doubled haploid (DH) populations obtained through two different methods, maize pollination (MP♀) and anther culture (AC♂). The comparison was based on a genetic linkage analysis, performed with DNA markers. Thirty-five polymorphic markers (15 SSR, 15 AFLP, 5 RAPD) were screened in MP♀ and AC♂ doubled haploids populations, derived from the same hybrid genotype (F1 of ‘Eta’ × ‘Darkhan 15’). Nine linkage groups, comprising 35 loci (the MP♀ lines) and 31 loci (the AC♂ lines), were constructed. The linkage groups in both DH populations showed identical orders of markers, except for one group mapping to chromosome 6B. The MP♀ and AC♂ linkage maps differed significantly in recombination frequencies for corresponding intervals. In total, the AC♂ linkage map (495.5 cM) was 40.5% longer than the MP♀ map (352.8 cM), indicating a significantly higher meiotic recombination rate in pollen mother cells. The enhancement in recombination was visible in five of nine linkage groups, and in 7 intervals between individual loci out of 19 compared. Moreover, for 6 other intervals a lack of linkage was observed in the AC♂ population, as compared to the MP♀ map.

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Acknowledgements

The authors are grateful to Dr. M. D. Gale (John Innes Centre, U.K.) for the SSR primers used in the study, and Prof. M. Maluszynski (Department of Genetics, Silesian University, Katowice) for critical reading of the manuscript. The work was supported by the Polish Committee of Scientific Research under a national grant PO6A 029 09, and by the International Atomic Energy Agency under Research Project 7719.

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  1. Department of Genetics, University of Silesia, Jagiellońska 28, Katowice, 40-032, Poland

    Justyna Guzy-Wróbelska, Anna Labocha-Pawlowska, Miroslaw Kwasniewski & Iwona Szarejko

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  1. Justyna Guzy-Wróbelska
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  2. Anna Labocha-Pawlowska
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  3. Miroslaw Kwasniewski
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  4. Iwona Szarejko
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Correspondence to Iwona Szarejko.

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Guzy-Wróbelska, J., Labocha-Pawlowska, A., Kwasniewski, M. et al. Different recombination frequencies in wheat doubled haploid populations obtained through maize pollination and anther culture. Euphytica 156, 173–183 (2007). https://doi.org/10.1007/s10681-007-9364-z

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  • DOI: https://doi.org/10.1007/s10681-007-9364-z

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