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Combined effects of ocean acidification and crude oil pollution on tissue damage and lipid metabolism in embryo–larval development of marine medaka (Oryzias melastigma)

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Abstract

Ocean acidification (OA) and crude oil pollution have been highlighted as some of the most pervasive anthropogenic influences on the ocean.In marine teleosts, early life-history stages are particularly vulnerable to disturbance by CO2-driven acidification as they lack pH-mediated intracellular regulation. Embryos exposed to trace levels of crude oil constituents dissolved in water exhibit a common syndrome of developmental abnormalities. So far, little is known about the combined effects of OA and crude oil on the early life history of marine fish. Eggs and larvae of the marine medaka (Oryzias melastigma) were treated with CO2 (1080 μatm atmospheric CO2), the water-soluble fraction (WSF) of crude oil (500 μg/L) and a CO2 (1080 μatm atmospheric CO2)/WSF (500 μg/L) mixture within 4 h after oviposition. Isolated and combined OA/WSF had no detectable effect on embryonic duration, egg survival rate and size at hatching. Histopathological anomalies of tissue and lipid metabolic disorder were significant when CO2 or WSF was given alone at 30 days of age. Combination of CO2 and WSF enhanced their toxicity compared to their separate administration. Since the early life-history stage of marine fish is thought to be impacted more heavily by increasing CO2 partial pressure (pCO2) levels and crude oil pollution, OA and crude oil pollution have the potential to act as an additional source of natural mortality.

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Acknowledgements

This work was supported by the Natural Science Foundation of Fujian Province of China (No. 2018J01067), the Program for Xiamen Southern Oceanographic Center (14GST69NF33), and the Ocean Public Welfare Scientific Research Special Appropriation Project (201405017); Professor John Hodgkiss of The University of Hong Kong is thanked for his help with English.

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  1. State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiang’an South Road, Xiamen, 361102, China

    Lingbin Sun, Jinpeng Ruan, Mengchao Lu, Meng Chen & Zhenghong Zuo

  2. Department of Anesthesiology, Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen, China

    Lingbin Sun & Zhongliang Dai

  3. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiang’an South Road, Xiamen, 361102, China

    Meng Chen

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Sun, L., Ruan, J., Lu, M. et al. Combined effects of ocean acidification and crude oil pollution on tissue damage and lipid metabolism in embryo–larval development of marine medaka (Oryzias melastigma). Environ Geochem Health 41, 1847–1860 (2019). https://doi.org/10.1007/s10653-018-0159-z

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