Skip to main content

Advertisement

SpringerLink
Log in

Microbiological reduction of hexavalent chromium by indigenous chromium-resistant bacteria in sand column experiments

  • Original Paper
  • Published:
Environmental Geochemistry and Health Aims and scope Submit manuscript

Abstract

Indigenous bacteria that are resistant to high concentrations of Cr(VI) were isolated from a Cr-contaminated sediment. Sand column experiments were conducted using the isolated bacteria to investigate microbial effects on Cr(VI) reduction in open systems that simulated subsurface conditions. The indigenous Cr-resistant bacteria appeared to reduce Cr(VI) in the column experiments. When 12 mg/L of Cr(VI) was injected into the columns, the removal efficiencies of Cr(VI) by the isolated bacteria were 39.1%, 62.5%, and 63.6% at 24, 48, and 72 h retention times of Cr(VI) solution, respectively. These results imply that the linear velocity of groundwater or pore water should be less than 0.63 cm/h for effective removal of Cr(VI) in subsurface conditions. In comparison, the noninoculated control column did not show a significant variation in dissolved Cr(VI) concentration. The results indicated that reduction of Cr(VI) was occurring in the column due to the activity of the indigenous bacteria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Cheung, K. H., & Gu, J. D. (2003). Reduction of chromate (CrO 2−4 ) by an enrichment consortium and an isolate of marine sulfate-reducing bacteria. Chemosphere, 52, 1523–1529.

    Article  CAS  Google Scholar 

  • Chirwa, E. M., & Wang, Y. T. (1997). Hexavalent chromium reduction by Bacillus sp. in a packed-bed bioreactor. Environmental Science and Technology, 31, 1446–1451.

    Article  CAS  Google Scholar 

  • Daniels, L., Hanson, R. S., & Phillips, J. A. (1994). Chemical analysis. In P. Gerhardt, R. G. E. Murray, W. A. Wood, & N. R. Krieg (Eds.), Methods for general and molecular bacteriology (pp. 512–554). Washington DC: ASM Press.

    Google Scholar 

  • Francis, C. A., Obraztsova, A. Y., & Tebo, B. M. (2000). Dissimilatory metal reduction by the facultative anaerobe Pantoea agglomerans SP1. Applied and Environmental Microbiology 66, 543–548.

    Article  CAS  Google Scholar 

  • Fukai, R. (1967). Valency state of chromium in seawater. Nature, 213, 901.

    Article  CAS  Google Scholar 

  • Guha, H., Jayachandran, K., & Maurrasse, F. (2001). Kinetics of chromium (VI) reduction by a type strain Shewanella alga under different growth conditions. Environmental Pollution, 115, 209–218.

    Article  CAS  Google Scholar 

  • Guha, H., Jayachandran, K., & Maurrasse, F. (2003). Microbiological reduction of chromium(VI) in presence of pyrolusite-coated sand by Shewanella alga Simidu ATCC 55627 in laboratory column experiments. Chemosphere, 52, 175–183.

    Article  CAS  Google Scholar 

  • Hedgecott, S. (1994). Prioritization and standards for hazardous chemicals. In P. Calow (Ed.), Handbook of ecotoxicology (pp. 378–382). Oxford: Blackwell Scientific.

    Google Scholar 

  • Lee, S. E., Lee J.-U., Lee J. S., & Chon H. T (2006). Effects of indigenous bacteria on Cr(VI) reduction in Cr-contaminated sediment with industrial wastes. Journal of Geochemical Exploration, 88, 41–44.

    Article  CAS  Google Scholar 

  • Lovley, D. R., & Phillips, E. J. P. (1994). Reduction of chromate by Desulfovibrio vulgaris and its C3 cytochrome. Applied and Environmental Microbiology, 60, 726–728.

    CAS  Google Scholar 

  • Michel, C., Brugna, M., Aubert, C., Bernadac, A., & Bruschi, M. (2001). Enzymatic reduction of chromate: Comparative studies using sulfate-reducing bacteria. Applied Microbiology and Biotechnology, 55, 95–100.

    Article  CAS  Google Scholar 

  • Shen, H., & Wang, Y. (1994). Biological reduction of chromium by E. coli. Journal of Environmental Engineering, 120, 560–572.

    Article  CAS  Google Scholar 

  • Tebo, B. M., & Obraztsova, A. Y. (1998). Sulfate-reducing bacterium grows with Cr(VI), U(VI), Mn(IV), and Fe(III) as electron acceptors. FEMS Microbiology Letters, 162, 193–198.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by Ministry of Environment and the Korea Institute of Environmental Science and Technology (KIEST) as “The Eco-Technopia 21 Project (2006–04001-0031-0)”.

Author information

Authors and Affiliations

  1. Research Institute of Engineering Science, Seoul National University, Seoul, 151-744, South Korea

    Sung-Eun Lee

  2. Department of Geosystem Engineering, Chonnam National University, Gwangju, 500-757, Korea

    Jong-Un Lee

  3. Department of Energy Resources Engineering, Seoul National University, Seoul, 151-744, Korea

    Hyo-Taek Chon

  4. Technology Research Center, Mine Reclamation Corporation, Seoul, 110-727, Korea

    Jin-Soo Lee

Authors
  1. Sung-Eun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jong-Un Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyo-Taek Chon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jin-Soo Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyo-Taek Chon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, SE., Lee, JU., Chon, HT. et al. Microbiological reduction of hexavalent chromium by indigenous chromium-resistant bacteria in sand column experiments. Environ Geochem Health 30, 141–145 (2008). https://doi.org/10.1007/s10653-008-9132-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10653-008-9132-6

Keywords

Search

Navigation