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Microencapsulation of nisin in alginate-cellulose nanocrystal (CNC) microbeads for prolonged efficacy against Listeria monocytogenes

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Abstract

The present study was undertaken to develop edible nisin-microencapsulated beads in order to inhibit growth of Listeria monocytogenes in ready-to-eat (RTE) ham. Different concentrations of nisin (16, 31, and 63 μg/ml) were microencapsulated into alginate-cellulose nanocrystal beads. Microencapsulation kept the available nisin (63 μg/ml) content 20 times greater compared with free nisin (63 μg/ml) during 28 days of storage at 4 °C. Results showed that 63 μg/ml microencapsulated nisin exhibited 31.26 μg/ml available nisin content after 28 days of storage at 4 °C, whereas there was no available nisin content left for free nisin. Cooked ham slices were then coated by the microencapsulated nisin beads, inoculated with L. monocytogenes [~3 log colony-forming units (CFU)/g], and stored at 4 °C under vacuum packaging for 28 days. The beads containing 16, 31, and 63 μg/ml nisin significantly (P ≤ 0.05) reduced the L. monocytogenes counts by 2.65, 1.50, and 3.04 log CFU/g after 28 days of storage compared with free nisin. Furthermore, microencapsulated nisin beads did not change the physicochemical properties (pH and color) of RTE ham during storage.

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Acknowledgments

This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and by FPInnovations (Pointe-Claire, Quebec, Canada) through the RDC program. The authors would also like to thank BSA Food Ingredients s.e.c./l.p. for providing salt ingredients (Montreal, Quebec, Canada). Tanzina Huq is the recipient of a scholarship from Fondation Universitaire Armand-Frappier.

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Authors and Affiliations

  1. Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre (CIC), INRS-Institut Armand-Frappier, University of Quebec, 531 Boulevard des Prairies, Laval, QC, H7V 1B7, Canada

    Tanzina Huq, Stephane Salmieri & Monique Lacroix

  2. Département des sciences du bois et de la forêt, Faculté de foresterie, géographie et géomatique, Université Laval, Quebec, QC, G1V 0A6, Canada

    Bernard Riedl

  3. FPInnovations, 570 Boulevard St-Jean, Pointe-Claire, QC, H9R 3J9, Canada

    Jean Bouchard

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  1. Tanzina Huq
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  2. Bernard Riedl
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  3. Jean Bouchard
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  4. Stephane Salmieri
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  5. Monique Lacroix
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Correspondence to Monique Lacroix.

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Huq, T., Riedl, B., Bouchard, J. et al. Microencapsulation of nisin in alginate-cellulose nanocrystal (CNC) microbeads for prolonged efficacy against Listeria monocytogenes . Cellulose 21, 4309–4321 (2014). https://doi.org/10.1007/s10570-014-0432-y

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