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Quantification of the activity of biomolecules in microarrays obtained by direct laser transfer

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Abstract

The direct-writing technique laser-induced forward transfer has been employed for the micro-array printing of liquid solutions of the enzyme horseradish peroxidase and the protein Titin on nitrocellulose solid surfaces. The effect of two UV laser pulse lengths, femtosecond and nanosecond has been studied in relation with maintaining the activity of the transferred biomolecules. The quantification of the active biomolecules after transfer has been carried out using Bradford assay, quantitative colorimetric enzymatic assay and fluorescence techniques. Spectrophotometric measurements of the HRP and the Titin activity as well as chromatogenic and fluorescence assay studies have revealed a connection between the properties of the deposited, biologically active biomolecules, the experimental conditions and the target composition. The bioassays have shown that up to 78% of the biomolecules remained active after femtosecond laser transfer, while this value reduced to 54% after nanosecond laser transfer. The addition of glycerol in a percentage up to 70% in the solution to be transferred has contributed to the stabilization of the micro-array patterns and the increase of their resolution.

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References

  • L.R. Allain, D.N. Stratis-Cullum, T. Vo-Dinh, Anal. Chim. Acta. 518, 77–85 (2004)

    Article  Google Scholar 

  • P. Arenkov, A. Kukhtin, A. Gemmell, S. Voloshchuk, V. Chupeeva, A. Mirzabekov, Anal. Biochem. 278, 123–131 (2000)

    Article  Google Scholar 

  • N.V. Avseenko, T.Y. Morozova, F.I. Ataullakhanov, V.N. Morozov, Anal. Chem. 73, 6047–6052 (2001)

    Article  Google Scholar 

  • J.A. Barron, B.R. Ringeisen, H. Kim, B.J. Spargo, D.B. Chrisey, Thin Solid Films 453–454, 383–387 (2004)

    Article  Google Scholar 

  • J.A. Barron, H.D. Young, D.D. Dlott, M.M. Darfler, D.B. Krizman, B.R. Ringeisen, Proteomics 5, 4138–4144 (2005a)

    Article  Google Scholar 

  • J.A. Barron, D.B. Krizman, B.R. Ringeisen, Ann. Biomed. Eng. 33, 121–130 (2005b)

    Article  Google Scholar 

  • S. Borini, M. Staiano, M. Rocchia, A.M. Rossi, M. Rossi, S. D’Auria, Recent Patents on DNA & Gene Sequences 1, 1–7 (2007)

    Article  Google Scholar 

  • M.M. Bradford, Anal. Biochem. 72, 248–254 (1976)

    Article  Google Scholar 

  • M. Colina, P. Serra, J.M. Fernández-Pradas, L. Sevilla, J.L. Morenza, Biosens. Bioelectron. 20, 1638–1642 (2005)

    Article  Google Scholar 

  • M. Colina, M. Duocastella, J.M. Fernández-Pradas, P. Serra, J.L. Morenza, J. Appl. Phys. 99(084909), 1–7 (2006)

    Google Scholar 

  • V. Dinca, M. Farsari, D. Kafetzopoulosc, A. Popescu, M. Dinescu, C. Fotakis, Thin Solid Films, (2008) in press DOI 10.1016/j.tsf.2008.02.043

  • J.M. Fernández-Pradas, M. Colina, P. Serra, J. Domínguez, J.L. Morenza, Thin Solid Films 453–454, 27–30 (2004)

    Article  Google Scholar 

  • A.P. Gunning, A.R. Mackie, P.J. Wilde, V.J. Morris, Surf. Interface Anal. 27, 433–436 (2004)

    Article  Google Scholar 

  • B. Hopp, T. Smausz, N. Kresz, N. Barna, Z. Bor, L. Kolozsvari, D.B. Chrisey, A. Szabo, A. Nogradi, Tissue Eng. 11, 1817–1823 (2005)

    Article  Google Scholar 

  • A. Karaiskou, I. Zergioti, C. Fotakis, M. Kapsetaki, D. Kafetzopoulos, Appl. Surf. Sci. 208–209, 245–249 (2003)

    Article  Google Scholar 

  • K.N. Lee, D.S. Shin, Y.S. Lee, Y.K. Kim, J. Micromech. Microeng. 13, 18–25 (2003)

    Article  Google Scholar 

  • A.R. Mackie, A.P. Gunning, P.J. Wilde, V.J. Morris, J. Colloid. Interface Sci. 210, 157–166 (1999)

    Article  Google Scholar 

  • B.D. Martin, B.P. Gaber, C.H. Patterson, D.C. Turner, Langmuir 14, 3971–3975 (1998)

    Article  Google Scholar 

  • M. Mrksich, G.M. Whitesides, Trends Biotechnol. 13, 228–235 (1995)

    Article  Google Scholar 

  • W. Norde, Adv. Colloid Interface Sci. 25(4), 267–340 (1986)

    Article  Google Scholar 

  • K. Prime, G. Whitesides, J. Am. Chem. Soc. 115, 10714–10721 (1993)

    Article  Google Scholar 

  • B.R. Ringeisen, P.K. Wu, H. Kim, A. Pique, R.Y.C. Auyeung, H.D. Young, D.B. Chrisey, D.B. Krizman, Biotechnol. Prog. 18, 1126–1129 (2002)

    Article  Google Scholar 

  • A. Roda, M. Guardigli, C. Russo, P. Pasini, M. Baraldini, BioTechniques 28, 492–496 (2000)

    Google Scholar 

  • S. Seong, C. Choi, Proteomics 3, 2176–2189 (2003)

    Article  Google Scholar 

  • P. Serra, M. Colina, J.M. Fernández-Pradas, L. Sevilla, J.L. Morenza, Appl. Phys. Lett. 85, 1639–1641 (2004)

    Article  Google Scholar 

  • P. Serra, J.M. Fernández-Pradas, M. Colina, M. Duocastella, J. Domínguez, J.L. Morenza, J. Laser Micro/Nanoeng. 1, 236–242 (2006)

    Google Scholar 

  • A. Sethuraman, M. Han, R. Kane, G. Belfor, Langmuir 20, 7779–7788 (2004)

    Article  Google Scholar 

  • D.B. Volkin, A.M. Klibanov, J. Biol. Chem. 262(7), 2945–2950 (1987) Mar

    Google Scholar 

  • I. Zergioti, D.G. Papazoglou, A. Karaiskou, C. Fotakis, E. Gamaly, A. Rode, Appl. Surf. Sci. 177, 208–209 (2003)

    Google Scholar 

  • I. Zergioti, A. Karaiskou, D.G. Papazoglou, C. Fotakis, M. Kapsetaki, D. Kafetzopoulos, Appl. Phys. Lett. 86, 163902 (2005)

    Article  Google Scholar 

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Acknowledgment

This work forms part of a research program funded by EU Marie Curie Fellowship Program: Advanced Training in Laser Sciences (MEST-CT-2004-008048), in IESL-FORTH, Heraklion, Crete, Greece.

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Authors and Affiliations

  1. Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), P.O. Box 527, 711 10, Heraklion, Crete, Greece

    V. Dinca, A. Ranella, M. Farsari & C. Fotakis

  2. Lasers Department, National Institute for Lasers, Plasma and Radiation Physics (NILPRP), Magurele, 077125, Bucharest, Romania

    V. Dinca & M. Dinescu

  3. Department of Crop Sciences, Technological Educational Institute of Crete, School of Agricultural Technology, Heraklion, Crete, Greece

    A. Ranella

  4. Institute of Molecular Biology and Biotechnology, Heraklion, Crete, Greece

    D. Kafetzopoulos

  5. Faculty of Physics, Bucharest University, Magurele, Bucharest, Romania

    A. Popescu

  6. Department of Physics, University of Crete, Heraklion, Crete, Greece

    C. Fotakis

Authors
  1. V. Dinca
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  2. A. Ranella
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  3. M. Farsari
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  4. D. Kafetzopoulos
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  5. M. Dinescu
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  6. A. Popescu
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  7. C. Fotakis
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Corresponding author

Correspondence to M. Farsari.

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Dinca, V., Ranella, A., Farsari, M. et al. Quantification of the activity of biomolecules in microarrays obtained by direct laser transfer. Biomed Microdevices 10, 719–725 (2008). https://doi.org/10.1007/s10544-008-9183-6

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  • DOI: https://doi.org/10.1007/s10544-008-9183-6

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