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Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells

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Abstract

We demonstrate the use of surface Zeta potential measurements as a new tool to investigate the interactions of iron oxide nanoparticles and cowpea mosaic virus (CPMV) nanoparticles with human normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) respectively. A substantial understanding in the interaction of nanoparticles with normal and cancer cells in vitro will enable the capabilities of improving diagnostic and treatment methods in cancer research, such as imaging and targeted drug delivery. A theoretical Zeta potential model is first established to show the effects of binding process and internalization process during the nanoparticle uptake by cells and the possible trends of Zeta potential change is predicted for different cell endocytosis capacities. The corresponding changes of total surface charge of cells in the form of Zeta potential measurements were then reported after incubated respectively with iron oxide nanoparticles and CPMV nanoparticles. As observed, after MCF7 and MCF10A cells were incubated respectively with two types of nanoparticles, the significant differences in their surface charge change indicate the potential role of Zeta potential as a valuable biological signature in studying the cellular interaction of nanoparticles, as well as specific cell functionality.

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Acknowledgements

Funding for this project provided by the Center of Excellence of Nanotechnology for Treatment, Understanding, and Monitoring of Cancer (NANO-TUMOR) has been gratefully acknowledged. We thank Dr. Maria Jose of the Moores Cancer Center at UC, San Diego (UCSD) for technical assistance in cell culture and Mr. Stephen McDaniel for preparing microtome cell slides for transmission electron microscopy. We also thank Dr. Sadik Esener and Michael Heller of UCSD for helpful discussions on cell surface charge.

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of California at Riverside, Riverside, CA, 92521, USA

    Yu Zhang & Cengiz S. Ozkan

  2. Department of Health Technology and Informatics, Hong Kong Polytechnic University, Kowloon, Hong Kong, China

    Mo Yang

  3. Department of Bioengineering, University of California at Riverside, Riverside, CA, 92521, USA

    Nathaniel G. Portney

  4. Department of Bio-Nano Science & Engineering, Shanghai JiaoTong University, Huashan Road 1954, Shanghai, 200030, China

    Daxiang Cui

  5. Gurer Budak Faculty of Medicine, Nanomedicine Research Laboratory, Gazi University, Besevler, Ankara, 06510, Turkey

    Gurer Budak

  6. Nanotechnology Research Center, Department of Physics and Department of Electrical Engineering, Bilkent University, Ankara, Turkey

    Ekmel Ozbay

  7. Department of Electrical Engineering, University of California at Riverside, Riverside, CA, 92521, USA

    Mihrimah Ozkan

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  1. Yu Zhang
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  2. Mo Yang
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  3. Nathaniel G. Portney
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  4. Daxiang Cui
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  5. Gurer Budak
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  8. Cengiz S. Ozkan
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Correspondence to Cengiz S. Ozkan.

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Zhang, Y., Yang, M., Portney, N.G. et al. Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells. Biomed Microdevices 10, 321–328 (2008). https://doi.org/10.1007/s10544-007-9139-2

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  • DOI: https://doi.org/10.1007/s10544-007-9139-2

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