biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:359-366, 2017 | DOI: 10.1007/s10535-016-0695-2

Differential proline metabolism in vegetative and reproductive tissues determine drought tolerance in chickpea

D. Kaur1, S. K. Grewal1,*, J. Kaur2, S. Singh2
1 Department of Biochemistry, Punjab Agricultural University, Ludhiana, India
2 Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India

Proline is emerging as a critical component of drought tolerance and fine tuning of its metabolism under stress affects the plants sensitivity and response to stress. Thus the study was carried out to analyse the effect of water deficit on the proline content and principal enzymes involved in its synthesis (Δ1-pyrolline-carboxylate synthetase) and catabolism (proline dehydrogenase) at different developmental stages and in different organs (roots, nodules, leaves, pod wall, and seeds) of two chickpea (Cicer arietinum L.) cultivars differing in drought tolerance (drought tolerant ICC4958 and drought sensitive ILC3279). It was observed that increased Δ1-pyrolline-carboxylate synthetase activity under moderate stress in roots and nodules of ICC4958 caused an increase in proline content during initiation of reproductive development whereas increased proline dehydrogenase activity in nodules and leaves at this period helped to maintain reducing power and energy supply in tissues and proper seed development as seed biomass increased consistently up to maturity. On the other hand, roots and nodules of ILC3279 responded to stress by increasing proline content after the developmental phase of reproductive organs was over (near maturity) which negatively affected the response of pod wall to stress. Concurrent increase in activities of Δ1-pyrolline-carboxylate synthetase and proline dehydrogenase in pod wall of ILC3279 aggravated the oxidative stress and affected seed development as seed biomass initially increased rapidly under stress but was unaffected near maturity.

Keywords: Cicer arietinum; Δ1-pyrolline-carboxylate synthetase; proline dehydrogenase; water stress
Subjects: proline metabolism; drought tolerance; growth parameter; pyrroline-5-carboxylate synthetase; proline dehydrogenase; water stress; chickpea

Received: November 26, 2015; Revised: May 31, 2016; Accepted: June 2, 2016; Published: June 1, 2017  Show citation

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Kaur, D., Grewal, S.K., Kaur, J., & Singh, S. (2017). Differential proline metabolism in vegetative and reproductive tissues determine drought tolerance in chickpea. Biologia plantarum61(2), 359-366. doi: 10.1007/s10535-016-0695-2
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