Skip to main content
SpringerLink
Log in

Simultaneous removal of hexavalent chromium and o-dichlorobenzene by isolated Serratia marcescens ZD-9

  • Original Paper
  • Published:
Biodegradation Aims and scope Submit manuscript

Abstract

Heavy metals–organics mixture pollution is increasingly concerned and simultaneous removal of organic pollutants and heavy metals is becoming significant. In this study, a strain was isolated from the sediment of a tannery effluent outfalls, which can remove o-dichlorobenzene (o-DCB) and Cr(VI) simultaneously. The bacterial isolate was identified as Serratia marcescens by the 16S rRNA gene sequences. The strain removed about 90% of o-DCB and more than 80% of Cr(VI) at the concentration of 1.29 g L−1 o-DCB and 20 mg L−1 Cr(VI). In the presence of concomitant pollutant o-DCB, the optimal pH (8.0) and temperature (30 °C) were determined for Cr(VI) removal. Changes of the bacterial cells and intracellular black Cr(III) sediments were observed by the TEM auxiliary analysis. The results of the FTIR spectroscopy analysis indicated that hydroxyl, amide and polysaccharides were involved in the process of Cr(VI) removal.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Albonetti S, Blasioli S, Bonelli R, Mengou JE, Scirè S, Trifirò F (2008) The role of acidity in the decomposition of 1,2-dichlorobenzene over TiO2-based V2O5/WO3 catalysts. Appl Catal A 341:18–25

    Article  CAS  Google Scholar 

  • Arjoon A, Olaniran AO, Pillay B (2013) Enhanced 1,2-dichloroethane degradation in heavy metal co-contaminated wastewater undergoing biostimulation and bioaugmentation. Chemosphere 93:1826–1834

    Article  CAS  Google Scholar 

  • Asatiani NV, Abuladze MK, Kartvelishvili TM, Bakradze NG, Sapojnikova NA, Tsibakhashvili NY, Tabatadze LV, Lejava LV, Asanishvili LL, Holman H-Y (2004) Effect of chromium(VI) action on Arthrobacter oxydans. Curr Microbiol 49:321–326

    Article  CAS  Google Scholar 

  • Bae WC, Lee H, Choe YC, Jahng DJ, Lee SH, Kim SJ, Lee JH, Jeong BC (2005) Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456. J Microbiol 43:21–27

    CAS  PubMed  Google Scholar 

  • Benning LG, Phoenix V, Yee N, Tobin M (2004) Molecular characterization of cyanobacterial silicification using synchrotron infrared micro-spectroscopy. Geochim Cosmochim Acta 68:729–741

    Article  CAS  Google Scholar 

  • Braeckevelt M, Mirschel G, Wiessner A, Rueckert M, Reiche N, Vogt C, Schultz A, Paschke H, Kuschk P, Kaestner M (2008) Treatment of chlorobenzene-contaminated groundwater in a pilot-scale constructed wetland. Ecol Eng 33:45–53

    Article  Google Scholar 

  • Campos VL, Moraga R, Yanez J, Zaror CA, Mondaca MA (2005) Chromate reduction by Serratia marcescens isolated from tannery effluent. Bull Environ Contam Toxicol 75:400–406

    Article  CAS  Google Scholar 

  • Cheng G, Li X (2009) Bioreduction of chromium(VI) by Bacillus sp. isolated from soils of iron mineral area. Eur J Soil Biol 45:483–487

    Article  CAS  Google Scholar 

  • Ganguli A, Tripathi A (2002) Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2 Chr in two bioreactors. Appl Microbiol Biotechnol 58:416–420

    Article  CAS  Google Scholar 

  • Garg SK, Tripathi M, Singh SK, Singh A (2013) Pentachlorophenol dechlorination and simultaneous Cr6+ reduction by Pseudomonas putida SKG-1 MTCC (10510): characterization of PCP dechlorination products, bacterial structure, and functional groups. Environ Sci Pollut Res 20:2288–2304

    Article  CAS  Google Scholar 

  • Gupta A, Balomajumder C (2015) Simultaneous removal of Cr(VI) and phenol from binary solution using Bacillus sp. immobilized onto tea waste biomass. J Water Process Eng 6:1–10

    Article  Google Scholar 

  • Han X, Wong YS, Wong MH, Tam NF (2007) Biosorption and bioreduction of Cr(VI) by a microalgal isolate, Chlorella miniata. J Hazard Mater 146:65–72

    Article  CAS  Google Scholar 

  • Kulkarni SG, Harris AJ, Casciano DA, Mehendale HM (1999) Differential protooncogene expression in Sprague Dawley and Fischer 344 rats during 1,2-dichlorobenzene-induced hepatocellular regeneration. Toxicology 139:119–127

    Article  CAS  Google Scholar 

  • Kumagai S, Matsunaga I (1997) Relations between exposure to o-dichlorobenzene and concentrations of urinary metabolites. J Occup Health 39:124–129

    Article  CAS  Google Scholar 

  • Lai CY, Zhong L, Zhang Y, Chen JX, Wen LL, Shi LD, Sun YP, Ma F, Rittmann BE, Zhou C, Tang Y, Zheng P, Zhao HP (2016) Bioreduction of chromate in a methane-based membrane biofilm reactor. Environ Sci Technol 50:5832–5839

    Article  CAS  Google Scholar 

  • Larrubia M (2002) An FT-IR study of the conversion of 2-chloropropane, o-dichlorobenzene and dibenzofuran on V2O5–MoO3–TiO2 SCR–DeNOx catalysts. Appl Catal B 39:343–352

    Article  CAS  Google Scholar 

  • Li J, Lin Q, Zhang X (2010) Mechanism of electron transfer in the bioadsorption of hexavalent chromium within Leersia hexandra Swartz granules by X-ray photoelectron spectroscopy. J Hazard Mater 182:598–602

    Article  CAS  Google Scholar 

  • Liu YG, Xu WH, Zeng GM, Li X, Gao H (2006) Cr(VI) reduction by Bacillus sp. isolated from chromium landfill. Process Biochem 41:1981–1986

    Article  CAS  Google Scholar 

  • Long D, Tang X, Cai K, Chen G, Chen L, Duan D, Zhu J, Chen Y (2013) Cr(VI) reduction by a potent novel alkaliphilic halotolerant strain Pseudochrobactrum saccharolyticum LY10. J Hazard Mater 256–257:24–32

    Article  Google Scholar 

  • Lou MM, Zhu B, Muhammad I, Li B, Xie GL, Wang YL, Li HY, Sun GC (2011) Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot pathogen Burkholderia seminalis. Carbohydr Res 346:1294–1301

    Article  CAS  Google Scholar 

  • Ma Z, Zhu W, Long H, Chai L, Wang Q (2007) Chromate reduction by resting cells of Achromobacter sp. Ch-1 under aerobic conditions. Process Biochem 42:1028–1032

    Article  CAS  Google Scholar 

  • Ma X, Sun Q, Feng X, He X, Guo J, Sun H, Cao H (2013) Catalytic oxidation of 1,2-dichlorobenzene over CaCO3/α-Fe2O3 nanocomposite catalysts. Appl Catal A 450:143–151

    Article  CAS  Google Scholar 

  • Mabbett AN, Macaskie LE (2001) A novel isolate of Desulfovibrio sp. with enhanced ability to reduce Cr(VI). Biotechnol Lett 23:683–687

    Article  CAS  Google Scholar 

  • Meena AK, Mishra G, Rai P, Rajagopal C, Nagar P (2005) Removal of heavy metal ions from aqueous solutions using carbon aerogel as an adsorbent. J Hazard Mater 122:161–170

    Article  CAS  Google Scholar 

  • Megharaj M, Avudainayagam S, Naidu R (2003) Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste. Curr Microbiol 47:51–54

    Article  CAS  Google Scholar 

  • Mohan D, Pittman CU Jr (2006) Activated carbons and low cost adsorbents for remediation of tri-and hexavalent chromium from water. J Hazard Mater 137(2):762–811

    Article  CAS  Google Scholar 

  • Monferran MV, Echenique JR, Wunderlin DA (2005) Degradation of chlorobenzenes by a strain of Acidovorax avenae isolated from a polluted aquifer. Chemosphere 61:98–106

    Article  CAS  Google Scholar 

  • Murugavelh S, Mohanty K (2012) Bioreduction of hexavalent chromium by free cells and cell free extracts of Halomonas sp. Chem Eng J 203:415–422

    Article  CAS  Google Scholar 

  • Olaniran AO, Balgobind A, Pillay B (2011) Quantitative assessment of the toxic effects of heavy metals on 1,2-dichloroethane biodegradation in co-contaminated soil under aerobic condition. Chemosphere 85:839–847

    Article  CAS  Google Scholar 

  • Patra RC, Malik S, Beer M, Megharaj M, Naidu R (2010) Molecular characterization of chromium(VI) reducing potential in Gram positive bacteria isolated from contaminated sites. Soil Biol Biochem 42:1857–1863

    Article  CAS  Google Scholar 

  • Pattanapipitpaisal P, Brown N, Macaskie L (2001) Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site. Appl Microbiol Biotechnol 57:257–261

    Article  CAS  Google Scholar 

  • Ramírez-Díaz MI, Díaz-Pérez C, Vargas E, Riveros-Rosas H, Campos-García J, Cervantes C (2008) Mechanisms of bacterial resistance to chromium compounds. Biometals 21(3):321–332

    Article  Google Scholar 

  • Shen G, Lu Y, Hong J (2006) Combined effect of heavy metals and polycyclic aromatic hydrocarbons on urease activity in soil. Ecotoxicol Environ Saf 63:474–480

    Article  CAS  Google Scholar 

  • Srivastava S, Ahmad AH, Thakur IS (2007) Removal of chromium and pentachlorophenol from tannery effluents. Bioresour Technol 98:1128–1132

    Article  CAS  Google Scholar 

  • Thacker U, Parikh R, Shouche Y, Madamwar D (2006) Hexavalent chromium reduction by Providencia sp. Process Biochem 41:1332–1337

    Article  CAS  Google Scholar 

  • Thompson I, Bailey M, Ellis R, Maguire N, Meharg A (1998) Response of soil microbial communities to single and multiple doses of an organic pollutant. Soil Biol Biochem 31:95–105

    Article  Google Scholar 

  • Tziotzios G, Dermou E, Politi D, Vayenas DV (2008) Simultaneous phenol removal and biological reduction of hexavalent chromium in a packed-bed reactor. J Chem Technol Biot 83:829–835

    Article  CAS  Google Scholar 

  • Vaiopoulou E, Gikas P (2012) Effects of chromium on activated sludge and on the performance of wastewater treatment plants: a review. Water Res 46:549–570

    Article  CAS  Google Scholar 

  • van der Meer JR, van Neerven AR, de Vries EJ, de Vos WM, Zehnder AJ (1991) Cloning and characterization of plasmid-encoded genes for the degradation of 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlorobenzene of Pseudomonas sp. strain P51. J Bacteriol 173:6–15

    Article  Google Scholar 

  • Viamajala S, Peyton BM, Apel WA, Petersen JN (2002) Chromate reduction in Shewanella oneidensis MR-1 is an inducible process associated with anaerobic growth. Biotechnol Prog 18:290–295

    Article  CAS  Google Scholar 

  • Viti C, Mini A, Ranalli G, Lustrato G, Giovannetti L (2006) Response of microbial communities to different doses of chromate in soil microcosms. Appl Soil Ecol 34:125–139

    Article  Google Scholar 

  • Wielgosiński G, Grochowalski A, Machej T, Pająk T, Ćwiąkalski W (2007) Catalytic destruction of 1,2-dichlorobenzene on V2O5–WO3/Al2O3–TiO2 catalyst. Chemosphere 67:S150–S154

    Article  Google Scholar 

  • Yee N, Benning LG, Phoenix VR, Ferris FG (2004) Characterization of metal–cyanobacteria sorption reactions: a combined macroscopic and infrared spectroscopic investigation. Environ Sci Technol 38:775–782

    Article  CAS  Google Scholar 

  • Zhong L, Lai CY, Shi LD, Wang KD, Dai YJ, Liu YW, Ma F, Rittmann BE, Zheng P, Zhao HP (2017) Nitrate effects on chromate reduction in a methane-based biofilm. Water Res 115:130–137

    Article  CAS  Google Scholar 

  • Ziagova M, Liakopoulou-Kyriakides M (2007) Comparison of cometabolic degradation of 1,2-dichlorobenzene by Pseudomonas sp. and Staphylococcus xylosus. Enzyme Microb Technol 40:1244–1250

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was financially supported by the Key Research and Development Program of Hunan Province (No. 2018SK2047), the National Natural Science Foundation of China (No. 51521006).

Author information

Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Weihua Xu, Guofeng Duan, Yunguo Liu, Guangming Zeng, Xin Li, Jie Liang & Wei Zhang

  2. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, People’s Republic of China

    Weihua Xu, Guofeng Duan, Yunguo Liu, Guangming Zeng, Xin Li, Jie Liang & Wei Zhang

Authors
  1. Weihua Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guofeng Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yunguo Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guangming Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jie Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Weihua Xu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, W., Duan, G., Liu, Y. et al. Simultaneous removal of hexavalent chromium and o-dichlorobenzene by isolated Serratia marcescens ZD-9. Biodegradation 29, 605–616 (2018). https://doi.org/10.1007/s10532-018-9856-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10532-018-9856-7

Keywords

Search

Navigation