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Isolation of Brazilian marine fungi capable of growing on DDD pesticide

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Abstract

The fungi Aspergillus sydowii Ce15, Aspergillus sydowii Ce19, Aspergillus sydowii Gc12, Bionectria sp. Ce5, Penicillium miczynskii Gc5, Penicillium raistrickii Ce16 and Trichoderma sp. Gc1, isolated from marine sponges Geodia corticostylifera and Chelonaplysylla erecta, were evaluated for their ability to grow in the presence of DDD pesticide. Increasing concentrations of DDD pesticide, i.e., 5.0 mg (1.56 × 10−12 mmol), 10.0 mg (3.12 × 10−2 mmol) and 15.0 mg (4.68 × 10−2 mmol) in solid and liquid culture media were tested. The fungi Trichoderma sp. Gc1 and Penicillium miczynskii Gc5 were able to grow in the presence of up to 15.0 mg of DDD, suggesting their potential for biodegradation. A 100% degradation of DDD was attained in liquid culture medium when Trichoderma sp. Gc1 was previously cultivated for 5 days and supplemented with 5.0 mg of DDD in the presence of hydrogen peroxide. However, the quantitative analysis showed that DDD was accumulated on mycelium and biodegradation level reached a maximum value of 58% after 14 days.

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Acknowledgments

The authors express their gratitude to Prof. Roberto G. S. Berlinck (IQSC-USP, São Carlos, SP, Brazil) for providing marine microorganisms. A.L.M. Porto thanks FAPESP (grant 2006/54401-2) and CNPq (grant 307830/2006-3) for their financial support. S.N. Ortega thanks FAPESP for her scholarship (grant 2007/58263-6).

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Authors and Affiliations

  1. Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador, São-carlense, 400, CP 780, CEP 13560-970, São Carlos, SP, Brazil

    Scarlet Nere Ortega, Marcia Nitschke, Ana Maria Mouad, Maria Diva Landgraf, Maria Olímpia Oliveira Rezende & André Luiz Meleiro Porto

  2. Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos, Via Washington Luís, Km 235, CEP 13565-905, São Carlos, SP, Brazil

    Mirna Helena Regali Seleghim

  3. Recursos de Divisão Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, CPQBA, Universidade Estadual de Campinas, Rua Alexandre Casellato, 999, CEP 13140-000, Paulínia, SP, Brazil

    Lara Durães Sette

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  1. Scarlet Nere Ortega
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  2. Marcia Nitschke
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  3. Ana Maria Mouad
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  4. Maria Diva Landgraf
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  5. Maria Olímpia Oliveira Rezende
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  6. Mirna Helena Regali Seleghim
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  7. Lara Durães Sette
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  8. André Luiz Meleiro Porto
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Correspondence to André Luiz Meleiro Porto.

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Ortega, S.N., Nitschke, M., Mouad, A.M. et al. Isolation of Brazilian marine fungi capable of growing on DDD pesticide. Biodegradation 22, 43–50 (2011). https://doi.org/10.1007/s10532-010-9374-8

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  • DOI: https://doi.org/10.1007/s10532-010-9374-8

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