Skip to main content
SpringerLink
Log in

RETRACTED ARTICLE: Immobilization of lactoperoxidase on ZnO nanoparticles with improved stability

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

This article was retracted on 16 April 2020

This article has been updated

Abstract

Objectives

The study aimed to develop a facile and effectual method to enhance the stability of lactoperoxidase (LPO) by immobilizing it on ZnO Nanoparticles (ZnO NPs).

Results

The successful immobilization of LPO on ZnO NPs was confirmed by using Fourier transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM). The Km values of free LPO and LPO immobilized on ZnO were 53.19, 89.28 mM and their Vmax values were 0.629, 0.46 µmol/mL min, respectively. The overall results showed that the stability of the immobilized LPO was significantly improved compared to free LPO. The LPO immobilized on ZnO (LPO–ZnO) retained 18% of the initial activity within 30 days at 25 °C whereas the free enzyme lost its activity after 7 days at the same temperature. Moreover, evaluation of the thermal stability of LPO at 75 °C determined the conservation of 12% of the initial activity of LPO in the LPO–ZnO sample after 60 min whereas the free enzyme lost its activity after 5 min.

Conclusions

According to the present results, ZnO nanoparticles are suitable for the immobilization of LPO.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

Change history

  • 16 April 2020

    The Editors have retracted this article [1] because significant parts of the text were duplicated from previously published articles by Ziyae et al. 2019 [2], Movahedi et al. 2019 [3] and Ziyae et al. 2018 [4]. The authors have not responded to any correspondence regarding this retraction.

References

  • Al-Baarri ANM, Legowo AM, Hayakawa S, Ogawa M (2015) Enhancement antimicrobial activity of hyphothiocyanite using carrot against Staphylococcus aureus and Escherichia coli. Procedia Food Sci 3:473–478

    Article  Google Scholar 

  • Ansari SA, Husain Q (2011) Immobilization of Kluyveromyces lactis β galactosidase on concanavalin A layered aluminium oxide nanoparticles—its future aspects in biosensor applications. J Mol Catal B Enzym 70:119–126

    Article  CAS  Google Scholar 

  • Benoy MJ, Essy AK, Sreekumar B, Haridas M (2000) Thiocyanate mediated antifungal and antibacterial property of goat milk lactoperoxidase. Life Sci 66(25):2433–2439

    Article  CAS  Google Scholar 

  • Borzouee F, Mofid MR, Varshosaz J, Samsam Shariat SZ (2016) Purification of lactoperoxidase from bovine whey and investigation of kinetic parameters. Adv Biomed Res 5:189–196

    Article  Google Scholar 

  • Chance B, Maehly A (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775

    Article  Google Scholar 

  • Davidson PM, Taylor TM, Schmidt SE (2013) Chemical preservatives and natural antimicrobial compounds. In: Doyle MP, Buchanan RL (eds) Food microbiology:fundamentals and frontiers, 4th edn. ASM Press, Washington, pp 765–801

    Google Scholar 

  • El-Fakharany EM, Uversky VN, Redwan EM (2017) Comparative analysis of the antiviral activity of camel, bovine, and human lactoperoxidases against herpes simplex virus type 1. Appl Biochem Biotechnol 182(1):294–310

    Article  CAS  Google Scholar 

  • Flemmig J, Gau J, Schlorke D, Arnhold J (2016) Lactoperoxidase as a potential drug target. Expert Opin Ther Targets 20(4):447–461

    Article  CAS  Google Scholar 

  • Gau J, Furtmuller PG, Obinger C, Prevost M, Van Antwerpen P, Arnhold J, Flemmig J (2016) Flavonoids as promoters of the (pseudo-) halogenating activity of lactoperoxidase and myeloperoxidase. Free Radic Biol Med 97:307–319

    Article  CAS  Google Scholar 

  • Ghorbani Gh, Ebrahimi M, Farhadyar N (2018) The immobilization of laccase enzyme from Trametes Versicolor on the surface of porous zinc oxide nanoparticles and studying features of the immobilized enzyme. Int J Bio-Inorg 7(1):21–28

    Google Scholar 

  • Gu X, Gao J, Li X, Wang Y (2018) Immobilization of Papain onto graphene oxide nanosheets. J Nanosci Nanotechnol 18(5):3543–3547

    Article  CAS  Google Scholar 

  • Husain Q, Ansari SA, Alam F, Azam A (2011) Immobilization of Aspergillus oryzae β galactosidase on zinc oxide nanoparticles via simple adsorption mechanism. Int J Biol Macromol 49(1):37–43

    Article  CAS  Google Scholar 

  • Hwang ET, Gu MB (2013) Enzyme stabilization by nano/microsized hybrid materials. Eng Life Sci 13(1):49–61

    Article  CAS  Google Scholar 

  • Jafary F, Kashanian S, Sharieat ZS, Jafary F, Omidfar K, Paknejad M (2012) Stability improvement of immobilized lactoperoxidase using polyaniline polymer. Mol Biol Rep 39(12):10407–10412

    Article  CAS  Google Scholar 

  • Kishore D, Talat M, Srivastava ON, Kayastha AM (2012) Immobilization of β-galactosidase onto functionalized graphene nanosheets using response surface methodology and its analytical applications. PLoS ONE 7(7):e40708

    Article  CAS  Google Scholar 

  • Kong J, Yu S (2007) Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin 39(8):549–559

    Article  CAS  Google Scholar 

  • Kussendrager KD, van Hooijdonk AC (2000) Lactoperoxidase: physico-chemical properties, occurrence, mechanism of action and applications. Br J Nutr 84(S1):19–25

    Article  Google Scholar 

  • Li M, Bala H, Lv X, Ma X, Sun F, Tang L, Wang Z (2007) Direct synthesis of monodispersed ZnO nanoparticles in an aqueous solution. Mater Lett 61:690–693

    Article  CAS  Google Scholar 

  • Lin HM, Tzeng SJ, Hsiau PJ, Tsai WL (1998) Electrode effects on gas sensing properties of nanocrystalline zinc oxide. Nanostruct Mater 10:465–477

    Article  CAS  Google Scholar 

  • Mecitoglu C, Yemenicioglu A (2007) Partial purification and preparation of bovine lactoperoxidase and characterization of kinetic properties of its immobilized form incorporated into crosslinked alginate films. Food Chem 104(2):726–733

    Article  CAS  Google Scholar 

  • Min K, Yoo YJ (2014) Recent progress in nanobiocatalysis for enzyme immobilization and its application. Biotechnol Bioprocess Eng 19:553–567

    Article  CAS  Google Scholar 

  • Mirouliaei M, Nayyeri H, Samsam SS, Movahedian AA (2007) Biospecific immobilization of lactoperoxidase on Con A-Sepharose 4B. Sci Iran 14:303–307

    Google Scholar 

  • Movahedi M, Samsam Shariat SZ, Nazem H (2019) Immobilization of lactoperoxidase on graphene oxide nanosheets and copper oxide nanoparticles and evaluation of their stability. Catal Lett 149(2):562–573

    Article  CAS  Google Scholar 

  • Nash RA, Haeger BE (1966) Zeta potential in the development of pharmaceutical suspensions. J Pharm Sci 55(8):829–837

    Article  CAS  Google Scholar 

  • Saleh A, Majed H, Yaaser Q, Ibrahim H (2017) Immobilization of horseradish peroxidase on Fe3O4 magnetic nanoparticles. Electron J Biotechnol 27:84–90

    Article  Google Scholar 

  • Samsam Shariat SZ, Borzouee F, Mofid MR, Varshosaz J (2018) Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability. Biotech Lett 40(9):1343–1353

    Article  Google Scholar 

  • Selvarajan E, Mohanasrinivasan V, Subathra Devi C, George Priya Doss C (2015) Immobilization of β-galactosidase from Lactobacillus plantarum HF571129 on ZnO nanoparticles: characterization and lactose hydrolysis. Bioprocess Biosyst Eng 38(9):1655–1669

    Article  CAS  Google Scholar 

  • Shah E, Mahapatra P, Bedekar AV, Soni HP (2015) Immobilization of Thermomyces lanuginosus lipase on ZnO nanoparticles: mimicking the interfacial environment. RSC Adv 5:26291–26300

    Article  CAS  Google Scholar 

  • Singh N, Srivastava G, Talat M, Raghubanshi H, Srivastava ON, Kayastha AM (2014) Cicer a-galactosidase immobilization onto functionalized graphene nanosheets using response surface method and its applications. Food Chem 142:430–438

    Article  CAS  Google Scholar 

  • Singhal M, Chhabra V, Kang P, Shah DO (1997) Synthesis of ZnO nanoparticles for varistor application using Zn-substituted aerosol ot microemulsion. Mater Res Bull 32:239–247

    Article  CAS  Google Scholar 

  • Stepankova V, Bidmanova S, Koudelakova T, Prokop Z, Chaloupkova R, Damborsky J (2013) Strategies for stabilization of enzymes in organic solvents. ACS Catal 3:2823–2836

    Article  CAS  Google Scholar 

  • Su R, Shi P, Zhu M, Hong F, Li D (2012) Studies on the properties of graphene oxide-alkaline protease bio-composites. Bioresour Technol 115:136–140

    Article  CAS  Google Scholar 

  • Sun H, Jin X, Jiang F, Zhang R (2018) Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO2 composites for the complete decolorization of anthraquinone dyes. Biotechnol Appl Biochem 65(2):220–229

    Article  CAS  Google Scholar 

  • Swarnalatha V, Esther RA, Dhamodharan R (2013) Immobilization of α-amylase on gum acacia stabilized magnetite nanoparticles, easily recoverable and reusable support. J Mol Catal B 96:6–13

    Article  CAS  Google Scholar 

  • Tenovuo J, Kurkijarvi K (1981) Immobilized lactoperoxidase as a biologically active and stable form of an antimicrobial enzyme. Arch Oral Biol 26(4):309–314

    Article  CAS  Google Scholar 

  • Vilian ATE, Chen SM, Kwak CH, Hwang SK, Huh YS, Han YK (2016) Immobilization of hemoglobin on functionalized multi-walled carbon nanotubes-poly-l-histidine-zinc oxide nanocomposites toward the detection of bromate and H2O2. Sens Actuators B 224:607–617

    Article  CAS  Google Scholar 

  • Wang RH, Xin JH, Tao XM, Daoud WA (2004) ZnO nanorods grown on cotton fabrics at low temperatures. Chem Phys Lett 398:250–255

    Article  CAS  Google Scholar 

  • Wang RH, Xin JH, Tao XM (2005) UV-blocking property of dumbbell-shaped ZnO crystallites on cotton fabrics. Inorg Chem 44:3926–3930

    Article  CAS  Google Scholar 

  • Yadav A, Virendra P, Kathe AA, Sheela R, Deepti Y, Sundaramoorthy C (2006) Functional finishing in cotton fabrics using zinc oxide nanoparticles. Bull Mater Sci 29:641–645

    Article  CAS  Google Scholar 

  • Zucca P, Sanjust E (2014) Inorganic materials as supports for covalent enzyme immobilization: methods and mechanisms. Molecules 19:14139–14194

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the Department of Biology, the Payame Noor University of Isfahan for their cooperation and supplying the experimental equipment.

Author information

Authors and Affiliations

  1. Department of Biology, Payame Noor University, P.O. Box 19395-4697, Tehran, Iran

    Mehrnaz Movahedi & Habibollah Nazem

  2. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

    Seyed Ziyae Aldin Samsam Shariat

  3. Department of Geology, Faculty of Earth Sciences, Shahid Beheshti University, Tehran, Iran

    Mehrdad Movahedi

Authors
  1. Mehrnaz Movahedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seyed Ziyae Aldin Samsam Shariat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Habibollah Nazem
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mehrdad Movahedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehrnaz Movahedi.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The Editors have retracted this article because significant parts of the text were duplicated from three previously published articles. The authors have not responded to any correspondence regarding this retraction.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Movahedi, M., Samsam Shariat, S.Z.A., Nazem, H. et al. RETRACTED ARTICLE: Immobilization of lactoperoxidase on ZnO nanoparticles with improved stability. Biotechnol Lett 42, 737–745 (2020). https://doi.org/10.1007/s10529-020-02828-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-020-02828-x

Keywords

Search

Navigation