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Accumulation of copper in Trichoderma reesei transformants, constructed with the modified Agrobacterium tumefaciens-mediated transformation technique

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Abstract

A modified Agrobacterium tumefaciens-mediated transformation method was established for the construction of mutants with improved copper tolerance and accumulation capability in Trichoderma reesei. One transformant, AT01, exhibited the highest copper accumulation capability. With copper at 0.7 mM, AT01 removed 13 mg copper/g biomass (removal rate of 96%), whereas the wild-type strain removed only 6 mg copper/g biomass (removal rate of 50%). Optimal conditions were a pH of 5.0 at 28°C. The pigment change of Trichoderma mycelia was a potential indicator of copper accumulation. Electron microscopy revealed that copper was mainly accumulated in cell vacuoles.

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Acknowledgments

This work was funded by the National High Technology Research and Development Program of China (2006AA10Z409), National Science and Technology Support Pro-255 gram (2006BAD08A06) and 948 Program of Agricultural Ministry of China (2006-G54 (A)), Governmental public industry research special funds for projects (200903052), Shanghai Science and Technology Commission Key Project (09391910900).

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Authors and Affiliations

  1. Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China

    Kehe Fu, Lixing Liu, Lili Fan, Tong Liu & Jie Chen

  2. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, 200240, People’s Republic of China

    Kehe Fu, Lixing Liu, Lili Fan, Tong Liu & Jie Chen

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  1. Kehe Fu
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  2. Lixing Liu
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  3. Lili Fan
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  4. Tong Liu
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  5. Jie Chen
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Correspondence to Jie Chen.

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Fu, K., Liu, L., Fan, L. et al. Accumulation of copper in Trichoderma reesei transformants, constructed with the modified Agrobacterium tumefaciens-mediated transformation technique. Biotechnol Lett 32, 1815–1820 (2010). https://doi.org/10.1007/s10529-010-0365-y

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  • DOI: https://doi.org/10.1007/s10529-010-0365-y

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