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Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta

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Abstract

A laccase was purified from Trametes hirsuta. This laccase was classified as a “white” or “yellow” laccase. pH 2.4 was optimal for the oxidation of ABTS and pH 2.5 for DMP. DMP oxidation was optimal at 85°C. The half-life of this laccase was 70 min at 75°C, and 5 h at 65°C. Non-phenolic dyes, such as Methyl Red, were oxidized by purified laccase without mediators. The enzyme was not inhibited by Cu2+, Mn2+, or EDTA. These are atypical laccase characteristics that make it a good candidate for theoretical and applied research.

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Acknowledgments

The authors are grateful to Dr. Roberta Greenwood for her assistance in editing this manuscript. This study was supported by the National science Foundation of China (Grant Nos. 30371136 and 30871985).

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China

    Zhang Haibo, Zhang Yinglong, Huang Feng & Gao Peiji

  2. Shandong Key Laboratory of Pulp and Paper Engineering, Shandong Institute of Light Industry, Jinan, 250353, China

    Huang Feng & Chen Jiachuan

Authors
  1. Zhang Haibo
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  2. Zhang Yinglong
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  3. Huang Feng
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  4. Gao Peiji
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  5. Chen Jiachuan
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Correspondence to Huang Feng.

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Haibo, Z., Yinglong, Z., Feng, H. et al. Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta . Biotechnol Lett 31, 837–843 (2009). https://doi.org/10.1007/s10529-009-9945-0

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  • DOI: https://doi.org/10.1007/s10529-009-9945-0

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