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Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats

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Abstract

Oral delivery of salmon calcitonin (sCT) to rats via a recombinant Saccharomyces cerevisiae was assessed. A synthetic sCT gene was cloned and expressed in S. cerevisiae yAGA2-sCT. Recombinant salmon calcitonin (rsCT) expression was detected by flow cytometry. The resorption activity of osteoclasts was inhibited by 3 × 10−6 M rsCT. Oral administration of 5 g lyophilized yAGA2-sCT/kg to hypercalcemic rats decreased serum calcium from 2.8 ± 0.02–2.7 ± 0.02 mM.

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Acknowledgements

This work was supported by the National ‘863’ Project of the State Science and Technology Commission of China (819-04-10).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, P.R. China

    Pingnan Sun, Xuecheng Zhang, Xiaonan Zang, Xiaoling Zhou, Yunsong Chen & K. K. I. U. Arunakumara

  2. Navy 401 Hospital of PLA, Qingdao, 266003, P.R. China

    Bin Liang

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  1. Pingnan Sun
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  2. Xuecheng Zhang
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  3. Xiaonan Zang
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  4. Xiaoling Zhou
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  5. Yunsong Chen
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  6. K. K. I. U. Arunakumara
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  7. Bin Liang
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Correspondence to Xuecheng Zhang.

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Sun, P., Zhang, X., Zang, X. et al. Anti-hypercalcemic effect of orally administered recombinant Saccharomyces cerevisiae expressing salmon calcitonin on hypercalcemic rats. Biotechnol Lett 29, 1013–1018 (2007). https://doi.org/10.1007/s10529-007-9352-3

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  • DOI: https://doi.org/10.1007/s10529-007-9352-3

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