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Biotechnologies in new high-throughput food allergy tests: why we need them

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Abstract

The increase in prevalence of food allergies generates a need for more accurate and reliable quantitative allergy testing in order to help diagnosis. In this short review, we briefly outline the history of food allergy testing and extend our comments to current multiplex techniques. Particular emphasis is given to new developments in the protein microarray area, where the use of recent advances in biotechnology has the potential to produce high-throughput devices with improved clinical significance.

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References

  • Aas K, Backman A, Belin L et al (1978) Standardization of allergen extracts with appropriate methods—combined use of skin prick testing and radio-allergosorbent tests. Allergy 33:130–137

    PubMed  CAS  Google Scholar 

  • Alcocer MJC, Murtagh GJ, Wilson PB et al (2004) The major human structural IgE epitope of the Brazil nut allergen Ber e 1: A chimaeric and protein microarray approach. J Mol Biol 343:759–769

    Article  PubMed  CAS  Google Scholar 

  • Arntz Y, Seelig JD, Lang HP et al (2003) Label-free protein assay based on a nanomechanical cantilever array. Nanotechnology 14:86–90

    Article  CAS  Google Scholar 

  • Bacarese-Hamilton TGJ, Ardizzoni A, Crisanti A (2005) Allergen microarrays. Method Mol Med 144:195–207

    Google Scholar 

  • Barry R, Soloviev M (2004) Quantitative protein profiling using antibody arrays. Proteomics 4:3717–3726

    Article  PubMed  CAS  Google Scholar 

  • Belov L, de la Vega O, dos Remedios CG et al (2001) Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer Res 61:4483–4489

    PubMed  CAS  Google Scholar 

  • Bertone P, Snyder M (2005) Advances in functional protein microarray technology. Febs J 272:5400–5411

    Article  PubMed  CAS  Google Scholar 

  • Blackley C (1959) Experimental researches on the causes and nature of Cartarrhus Aestivus (Hayfever or Hay-Asthma). Dawson’s of Pall Mall

  • Dyukova VI, Shilova NV, Galanina OE et al (2006) Design of carbohydrate multiarrays. Biochim Biophys Acta—General Subjects 1760:603–609

    Article  CAS  Google Scholar 

  • Fall BI, Eberlein-Konig B, Behrendt H et al (2003) Microarrays for the screening of allergen-specific IgE in human serum. Anal Chem 75:556–562

    Article  PubMed  CAS  Google Scholar 

  • Geierstanger BH, Saviranta P, Brinker A (2006) Antibody microarrays using resonance light-scattering particles for detection. Methods Mol Biol 328:31–50

    PubMed  CAS  Google Scholar 

  • Haab BB, Lizardi PM (2006) RCA-enhanced protein detection arrays. Methods Mol Biol 328:15–29

    PubMed  CAS  Google Scholar 

  • Haab BB (2003) Methods and applications of antibody microarrays in cancer research. Proteomics 3:2116–2122

    Article  PubMed  CAS  Google Scholar 

  • Harwanegg C, Hiller R (2004) Protein microarrays in diagnosing IgE-mediated diseases: spotting allergy at the molecular level. Exp Rev Mol Diagn 4:539–548

    Article  CAS  Google Scholar 

  • Harwanegg C, Hiller R (2005) Protein microarrays for the diagnosis of allergic diseases: state-of-the-art and future development. Clin Chem Lab Med 43:1321–1326

    Article  PubMed  CAS  Google Scholar 

  • Harwanegg C, Laffer S, Hiller R et al (2003) Microarrayed recombinant allergens for diagnosis of allergy. Clin Exp Allergy 33:7–13

    Article  PubMed  CAS  Google Scholar 

  • Hashmi G, Shariff T, Seul M et al (2005) A flexible array format for large-scale, rapid blood group DNA typing. Transfusion 45:680–688

    Article  PubMed  CAS  Google Scholar 

  • Hiller R, Laffer S, Harwanegg C et al (2002) Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB J 16:U262–U282

    Google Scholar 

  • Huang JX, Mehrens D, Wiese R et al (2001) High-throughput genomic and proteomic analysis using microarray technology. Clin Chem 47:1912–1916

    PubMed  CAS  Google Scholar 

  • Ishizaka K, Ishizaka T (1967) Identification of gamma-E-antibodies as a carrier of reaginic activity. J Immunol 99:1187–1198

    Google Scholar 

  • Jahn-Schmid B, Harwanegg C, Hiller R et al (2003) Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin E. Clin Exp Allergy 33:1443–1449

    Article  PubMed  CAS  Google Scholar 

  • Kim TE, Park SW, Cho NY et al (2002) Quantitative measurement of serum allergen-specific IgE on protein chip. Exp Mol Med 34:152–158

    PubMed  CAS  Google Scholar 

  • Kingsmore SF, Patel DD (2003) Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification. Curr Opin Biotechnol 14:74–81

    Article  PubMed  CAS  Google Scholar 

  • Kukar T, Eckenrode S, Gu YR et al (2002) Protein microarrays to detect protein–protein interactions using red and green fluorescent proteins. Anal Biochem 306:50–54

    Article  PubMed  CAS  Google Scholar 

  • Kusnezow W, Hoheisel JD (2003) Solid supports for microarray immunoassays. J Mol Recogn 16:165–176

    Article  CAS  Google Scholar 

  • Lebrun SJ, Petchpud WN, Hui A et al (2005) Development of a sensitive, colorometric microarray assay for allergen-responsive human IgE. J Immunol Methods 300:24–31

    Article  PubMed  CAS  Google Scholar 

  • Lee KB, Park SJ, Mirkin CA et al (2002) Protein nanoarrays generated by dip-pen nanolithography. Science 295:1702–1705

    Article  PubMed  CAS  Google Scholar 

  • MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput function determination. Science 289:1760–1763

    PubMed  CAS  Google Scholar 

  • Madsen C (2005) Prevalence of food allergy: an overview. Proc Nutr Soc 64:413–417

    Article  PubMed  Google Scholar 

  • Merkel JS, Michaud GA, Salcius M et al (2005) Functional protein microarrays: just how functional are they? Curr Opin Biotechnol 16:447–452

    Article  PubMed  CAS  Google Scholar 

  • Molloy RM, Mc Connell RI, Lamont JV et al (2005) Automation of biochip array technology for quality results. Clin Chem Lab Med 43:1303–1313

    Article  PubMed  CAS  Google Scholar 

  • Moody MD, Van Arsdell SW, Murphy KP, Orencole SF, Burns C (2001) Array-based ELISAs for high-throughput analysis of human cytokines. Biotechniques 31:186–190, 192–194

    Google Scholar 

  • Nielsen UB, Cardone MH, Sinskey AJ et al (2003) Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci USA 100:9330–9335

    Article  PubMed  Google Scholar 

  • Nielsen UB, Geierstanger BH (2004) Multiplexed sandwich assays in microarray format. J Immunol Methods 290:107–120

    Article  PubMed  CAS  Google Scholar 

  • Pavlickova P, Schneider EM, Hug H (2004) Advances in recombinant antibody microarrays. Clin Chim Acta 343:17–35

    Article  PubMed  CAS  Google Scholar 

  • Predki PF (2004) Functional protein microarrays: ripe for discovery. Curr Opin Chem Biol 8:8–13

    Article  PubMed  CAS  Google Scholar 

  • Sampson HA (2004) Update on food allergy. J Allergy Clin Immunol 113:805–819

    Article  PubMed  CAS  Google Scholar 

  • Sampson HA (2005) Food allergy—accurately identifying clinical reactivity. Allergy 60:19–24

    Article  PubMed  Google Scholar 

  • Sasakura Y, Kanda K, Fukuzono S (2006) Microarray techniques for more rapid protein quantification: use of single spot multiplex analysis and a vibration reaction unit. Anal Chim Acta 564:53–58

    Article  CAS  Google Scholar 

  • Saviranta P, Okon R, Brinker A et al (2004) Evaluating sandwich immunoassays in microarray format in terms of the ambient analyte regime. Clin Chem 50:1907–1920

    Article  PubMed  CAS  Google Scholar 

  • Schweitzer B, Roberts S (1998) Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat Biotechnol 20:359–365

    Article  Google Scholar 

  • Schweitzer B, Roberts S, Grimwade B et al (2002) Multiplexed protein profiling on microarrays by rolling-circle amplification. Nat Biotechnol 20:359–365

    Article  PubMed  CAS  Google Scholar 

  • Seltzer JM, Halpern GM, Tsay YG (1984) Correlation of allergy test-results obtained by IgE fast, rast, and prick-puncture methods. Ann Allergy 52:240–240

    Google Scholar 

  • Shreffler WG, Lencer DA, Bardina L et al (2005) IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2. J Allergy Clin Immunol 116:893–899

    Article  PubMed  CAS  Google Scholar 

  • Steinhauer C, Ressine A, Marko-Varga G et al (2005) Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates. Anal Biochem 341:204–213

    Article  PubMed  CAS  Google Scholar 

  • Uttamchandani M, Walsh DP, Yao SQ et al (2005) Small molecule microarrays: recent advances and applications. Curr Opin Chem Biol 9:4–13

    Article  PubMed  CAS  Google Scholar 

  • Wang CC, Huang RP, Sommer M et al (2002) Array-based multiplexed screening and quantitation of human cytokines and chemokines. J Proteome Res 1:337–343

    Article  PubMed  CAS  Google Scholar 

  • Wide L, Bennich H, Johansson SG (1967) Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet 2:1105–1107

    Google Scholar 

  • Wiese R, Belosludtsev Y, Powdrill T et al (2001) Simultaneous multianalyte ELISA performed on a microarray platform. Clin Chem 47:1451–1457

    PubMed  CAS  Google Scholar 

  • Wodicka L, Dong H, Mittmann M et al (1997) Genome-wide expression monitoring in Saccharomyces cerevisiae. Nat Biotechnol 15:1359–1367

    Article  PubMed  CAS  Google Scholar 

  • Woodbury RL, Varnum SM, Zangar RC (2002) Elevated HGF levels in sera from breast cancer patients detected using a protein microarray ELISA. J Proteome Res 1:233–277

    Article  PubMed  CAS  Google Scholar 

  • Yguerabide J, Yguerabide EE (2001) Resonance light scattering particles as ultrasensitive labels for detection of analytes in a wide range of applications. J Cell Biochem Suppl 37:71–81

    Article  PubMed  Google Scholar 

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Correspondence to Marcos J. C. Alcocer.

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Renault, N.K., Mirotti, L. & Alcocer, M.J.C. Biotechnologies in new high-throughput food allergy tests: why we need them. Biotechnol Lett 29, 333–339 (2007). https://doi.org/10.1007/s10529-006-9251-z

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  • DOI: https://doi.org/10.1007/s10529-006-9251-z

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