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Direct Electrochemical Immunoassay Based on Immobilization of Protein-Magnetic Nanoparticle Composites on to Magnetic Electrode Surfaces by Sterically Enhanced Magnetic Field Force

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Abstract

A direct electrochemical immunoassay system based on the immobilization of α-1-fetoprotein antibody (anti-AFP), as a model system, on the surface of core-shell Fe2O3/Au magnetic nanoparticles (MNP) has been demonstrated. To fabricate such an assay system, anti-AFP was initially covalently immobilized on to the surface of core-shell Fe2O3/Au MNP. Anti-AFP-modified MNP (bio-nanoparticles) were then attached to the surface of carbon paste electrode with the aid of a permanent magnet. The performance and factors influencing the performance of the resulting immunosensor were studied. α-1-Fetoprotein antigen was directly determined by the change in current or potential before and after the antigen–antibody reaction versus saturated calomel electrode. The electrochemical immunoassay system reached 95% of steady-state potential within 2 min and had a sensitivity of 25.8 mV. The linear range for AFP determination was from 1 to 80 ng AFP ml−1 with a detection limit of 0.5 ng AFP ml−1. Moreover, the direct electrochemical immunoassay system, based on a functional MNP, can be developed further for DNA sensor and enzyme biosensor.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry (Chongqing), College of Chemistry and Chemical Engineering, Southwest University, 400700, Chongqing, P.R. China

    Dianping Tang, Ruo Yuan & Yaqin Chai

Authors
  1. Dianping Tang
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  2. Ruo Yuan
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  3. Yaqin Chai
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Corresponding author

Correspondence to Ruo Yuan.

Additional information

Revisions requested 2 November 2005; Revisions received 17 January 2006

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Tang, D., Yuan, R. & Chai, Y. Direct Electrochemical Immunoassay Based on Immobilization of Protein-Magnetic Nanoparticle Composites on to Magnetic Electrode Surfaces by Sterically Enhanced Magnetic Field Force. Biotechnol Lett 28, 559–565 (2006). https://doi.org/10.1007/s10529-006-0017-4

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  • DOI: https://doi.org/10.1007/s10529-006-0017-4

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