Skip to main content
Log in

Head group specificity of phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.)

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The biocatalytical potential of two new phospholipase D (PLD) isoenzymes from poppy seedlings (Papaver somniferum L.), PLD-A and PLD-B, was examined by comparing their activities in phospholipid transformation. Both enzymes showed the same ratio in rates of hydrolysis [phosphatidylcholine (PC):phosphatidylglycerol (PG):phosphatidylserine:phosphatidylinositol=1:0.5:0.3:0.1] and were inactive towards phosphatidylethanolamine (PE). PLD-A did not catalyze head group exchange whereas PLD-B showed a high transphosphatidylation potential in the conversion of PC into PG and PE. This enzyme also catalyzed the transesterification of octadecylphosphocholine into octadecylphosphoglycerol or octadecylphosphoethanolamine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • I Aurich P Dürrschmidt A Schierhorn R Ulbrich-Hofmann (2002) ArticleTitleProduction of octadecylphospho- l-serine by phospholipase D Biotechnol. Lett. 24 585–590 Occurrence Handle10.1023/A:1014876914801 Occurrence Handle1:CAS:528:DC%2BD38Xjt1Gmu70%3D

    Article  CAS  Google Scholar 

  • Brachwitz H, Schönfeld R, Langen P, Paltauf F, Hermetter A (1989) New alkylphosphono- and phosphoserines, method for preparing them, and pharmaceutical substances containing them. World Patent PCT WO 89/10370.

  • P D’Arrigo L Ferra Particlede V Piergianni A Selva S Servi A Strini (1996) ArticleTitlePreparative transformation of natural phospholipids catalysed by phospholipase D from Streptomyces J. Chem. Soc., Perkin Trans. 1 2651–2656 Occurrence Handle10.1039/p19960002651

    Article  Google Scholar 

  • RMC Dawson (1967) ArticleTitleThe formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D Biochem. J. 102 205–210 Occurrence Handle1:CAS:528:DyaF2sXhs1Ohsw%3D%3D

    CAS  Google Scholar 

  • JH Dyer SB Ryu X Wang (1994) ArticleTitleMultiple forms of phospholipase D following germination and during leaf development of castor bean Plant Physiol. 105 715–724 Occurrence Handle1:CAS:528:DyaK2cXksFCjtLc%3D

    CAS  Google Scholar 

  • H Eibl S Kovatchev (1981) ArticleTitlePreparation of phospholipids and their analogs by phospholipase D Meth. Enzymol. 72 632–639 Occurrence Handle10.1016/S0076-6879(81)72055-X Occurrence Handle1:CAS:528:DyaL38XmsFSgtg%3D%3D

    Article  CAS  Google Scholar 

  • JH Exton (2002) ArticleTitlePhospholipase D–structure, regulation and function Rev. Physiol. Biochem. Pharmacol. 144 1–94 Occurrence Handle1:CAS:528:DC%2BD38XivFyisbY%3D Occurrence Handle10.1007/BFb0116585

    Article  CAS  Google Scholar 

  • P Hilgard T Klenner J Stekar C Unger (1993) ArticleTitleAlkylphosphocholines: a new class of membrane-active anticancer agents Cancer Chemother. Pharmacol. 23 90–95 Occurrence Handle10.1007/BF00685608

    Article  Google Scholar 

  • F Hirche MHJ Koch S Konig T Wadewitz R Ulbrich-Hofmann (1997) ArticleTitleThe influence of organic solvents on phospholipid transformations by phospholipase D in emulsion system Enzyme Microb. Technol. 20 453–461 Occurrence Handle10.1016/S0141-0229(97)00172-5 Occurrence Handle1:CAS:528:DyaK2sXislajtrs%3D

    Article  CAS  Google Scholar 

  • F Hirche R Ulbrich-Hofmann (1999) ArticleTitleThe interfacial pressure is an important parameter for the rate of phospholipase D catalyzed reactions in emulsion systems Biochim. Biophys. Acta 1436 383–389 Occurrence Handle1:CAS:528:DyaK1MXovFynsQ%3D%3D

    CAS  Google Scholar 

  • F Hirche R Ulbrich-Hofmann (2000) ArticleTitleThe interdependence of solvent, acceptor alcohol and enzyme source in transphosphatidylation by phospholipase D Biocatal. Biotrans. 18 343–353 Occurrence Handle1:CAS:528:DC%2BD3cXnsFOms74%3D Occurrence Handle10.3109/10242420009015256

    Article  CAS  Google Scholar 

  • SB Mandal PC Sen P Chakrabarti (1980) ArticleTitle In vitro synthesis of phosphatidylinositol and phosphatidylcholine by phospholipase D Phytochemistry 19 1661–1663 Occurrence Handle10.1016/S0031-9422(00)83789-9 Occurrence Handle1:CAS:528:DyaL3cXmtlCnt74%3D

    Article  CAS  Google Scholar 

  • M Oblozinsky R Schoeps R Ulbrich-Hofmann L Bezakova (2003) ArticleTitleTwo uncommon phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.) Biochim. Biophys. Acta 1631 153–159 Occurrence Handle1:CAS:528:DC%2BD3sXhvFaisbw%3D

    CAS  Google Scholar 

  • K Pappan S Austin-Brown KD Chapman X Wang (1998) ArticleTitleSubstrate selectivities and lipid modulation of plant phospholipase Dα, -β, and -γ Arch. Biochem. Biophys. 353 131–140 Occurrence Handle10.1006/abbi.1998.0640 Occurrence Handle1:CAS:528:DyaK1cXjtF2ktrw%3D

    Article  CAS  Google Scholar 

  • S Servi (1999) Phospholipases as synthetic catalysts D Fessner (Eds) Topics in Current Chemistry (Biocatalysis from Discovery to Application) Springer-Verlag Berlin 127–158 Occurrence Handle10.1007/3-540-68116-7_5

    Chapter  Google Scholar 

  • M Takami N Hidaka Y Suzuki (1994) ArticleTitlePhospholipase D-catalyzed synthesis of phosphatidyl aromatic compounds Biosci. Biotechnol. Biochem. 58 2140–2144 Occurrence Handle1:CAS:528:DyaK2MXjtFWruro%3D Occurrence Handle10.1271/bbb.58.2140

    Article  CAS  Google Scholar 

  • R Ulbrich-Hofmann (2000) Phospholipases used in lipid transformations UT Bornscheuer (Eds) Enzymes in Lipid Modification Wiley-VCH Weinheim 219–262

    Google Scholar 

  • R Ulbrich-Hofmann (2003) ArticleTitleEnzyme-catalysed transphosphatidylation Eur. J. Lipid Sci. Technol. 105 305–307 Occurrence Handle10.1002/ejlt.200390060 Occurrence Handle1:CAS:528:DC%2BD3sXltFarsbw%3D

    Article  CAS  Google Scholar 

  • X Wang (2000) ArticleTitleMultiple forms of phospholipase D in plants: the gene family, catalytic and regulatory properties, and cellular functions Prog. Lipid Res. 39 109–149 Occurrence Handle10.1016/S0163-7827(00)00002-3 Occurrence Handle1:CAS:528:DC%2BD3cXjslSqtbk%3D

    Article  CAS  Google Scholar 

  • CH Yu SY Liu V Panagia (1996) ArticleTitleThe transphosphatidylation activity of phospholipase D Mol. Cell. Biochem. 157 101–105 Occurrence Handle10.1007/BF00227886 Occurrence Handle1:CAS:528:DyaK28Xjt1arsbk%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. Ulbrich-Hofmann.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oblozinsky, M., Ulbrich-Hofmann, R. & Bezakova, L. Head group specificity of phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.). Biotechnol Lett 27, 181–185 (2005). https://doi.org/10.1007/s10529-004-7853-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-004-7853-x

Keywords

Navigation