Abstract
An improved method to transform nucleosides into 5′-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by Zn2+. Under optimal conditions, the extent of nucleoside transformations into nucleoside-5′-monophosphates were 41–91% (mol).
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Revisions requested 22 September 2004; Revisions received 11 October 2004
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Barai, V.N., Kvach, S.V., Zinchenko, A.I. et al. An improved method for the enzymatic transformation of nucleosides into 5′-monophosphates. Biotechnol Lett 26, 1847–1850 (2004). https://doi.org/10.1007/s10529-004-5311-4
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DOI: https://doi.org/10.1007/s10529-004-5311-4