Abstract
This study investigated the effectiveness of Trichoderma asperellum (Samuels, Lieckf. & Nirenberg) and Metarhizium anisopliae (Metschn.) Sorokin in controlling pepper powdery mildew. Culture extracts of these fungi inhibited conidial germination in Leveillula taurica (Lév.) G. Arnaud. T. asperellum showed higher chitinolytic activity than that of M. anisopliae. The latter exhibited elevated β-1,3-glucanase activity as compared with that of T. asperellum. In a high-tunnel greenhouse, T. asperellum was more persistent than M. anisopliae. Jalapeño pepper was more vulnerable to powdery mildew than arbol pepper. In the field, the fungi did not control powdery mildew in jalapeño pepper. In arbol pepper, both fungal species reduced disease severity to a level similar to that of a chemical control. T. asperellum and M. anisopliae may be effective in the control of powdery mildew on peppers and could lead to the disuse of pesticides on some but not all types of pepper.
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References
Barajas CG, del Pozo EM, García I, Méndez A (2010) Obtención de conidios del aislamiento Ma-002 de Metarhizium anisopliae (Metsch.) Sorokin mediante una alternativa de cultivo bifásico. Rev Prot Veg 25:174–180
Brand M, Messika Y, Elad Y, David DR, Sztejnberg A (2009) Spray treatments combined with climate modification for the management of Leveillula taurica in sweet pepper. Eur J Plant Pathol 124:309–329
Castrillo LA, Griggs MH, Liu H, Bauer LS, Vandenberg JD (2010) Assessing deposition and persistence of Beauveria bassiana GHA (Ascomycota: Hypocreales) applied for control of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), in a commercial tree nursery. Biol Control 54:61–67
Deore PB, Sawant DM (2001) Management of guar powdery mildew by Trichoderma spp. culture filtrates. J Maharashtra Agric Univ 25:253–254
Fernández OD, Torés JA, De Vicente A, Pérez GA (2008) Field resistance to QoI fungicides in Podosphaera fusca is not supported by typical mutations in the mitochondrial cytochrome b gene. Pest Manag Sci 64:694–702
Freimoser MF, Hu G, St Leger RJ (2005) Variation in gene expression patterns as the insect pathogen Metarhizium anisopliae adapts to different host cuticles or nutrient deprivation in vitro. Microbiology 151:361–371
Gao Q, Jin K, Ying S, Zhang Y, Xiao G, Shang Y, Duan Z, Hu X, Xie X, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang S, Zhong Y, Ma L, St Leger RJ, Zhao G, Pei Y, Feng M, Xia Y, Wang C (2011) Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genet 7(1):e1001264
Guigón LC, Guerrero PV, Vargas AF, Carvajal ME, Ávila QGD, Bravo LL, Ruocco M, Lanzuise S, Woo S, Lorito M (2010) Identificación molecular de cepas nativas de Trichoderma spp. Su tasa de crecimiento in vitro y antagonismo contra hongos fitopatogenos. Rev Mex de Fitopatol 28:87–96
Guigón LC, Guerrero PV, Lanzuise S, Lorito M (2014) Enzyme activity of extracellular protein induced in Trichoderma asperellum and T. longibrachiatum by substrates based on Agaricus bisporus and Phymatotrichopsis omnivora. Fungal Biol 118:211–221
Guigón LC, Vargas AF, Guerrero PV, Ruocco M, Lorito M (2015) Changes in Trichoderma asperellum enzyme expression during parasitism of the cotton root rot pathogen Phymatotrichopsis omnivora. Fungal Biol 119:264–273
Guzmán VP, Bravo LL, Montes BR, Guigón LC, Sepúlveda JG (2014) Induction of resistance to Sclerotium rolfsii in different varieties of onion by inoculation with Trichoderma asperellum. Eur J Plant Pathol 138:223–229
Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56
Kiss L (2003) A review of fungal antagonists of powdery mildews and their potential as biocontrol agents. Pest Manag Sci 59:475–483
Mukherjee PK, Horwitz BA, Kenerley CM (2012) Secondary metabolism in Trichoderma a genomic perspective. Microbiology 158:35–45
Ownley BH, Gwinn KD, Vega FE (2010) Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl 55:113–128
Pertot I, Zasso R, Amsalem L, Baldessari M, Angeli G, Elad Y (2004) Use of biocontrol agents against powdery mildew in integrated strategies for reducing pesticide residues on strawberry: evaluation of efficacy and side effects. Management of plant diseases and arthropod pests by BCAs. IOBC/WPRS Bull 27:109–113
Petlamu WL, Prasertsan P (2012) Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity. Mycobiology 40:111–116
Seidl V, Song L, Lindquist E, Gruber S, Koptchinskiy A, Zeilinger S, Schmoll M, Martínez P, Sun J, Grigoriev I, Herrera EA, Baker SE, Kubicek CP (2009) Transcriptomic response of the mycoparasitic fungus Trichoderma atroviride to the presence of a fungal prey. BMC Genomics 10:567
Sudha A, Lakshmanan P (2009) Integrated disease management of powdery mildew (Leveillula taurica (Lev.) Arn.) of chilli (Capsicum annuum L.). Arch Phytopathol Plant Prot 42:299–317
Tsror L, Lebiush MS, Shapira N (2004) Control of powdery mildew on organic pepper. Management of plant diseases and arthropod pests by BCAs. IOBC/WPRS Bull 27:333–336
Tucci M, Ruocco M, De Masi L, De Palma L, Lorito M (2011) The beneficial effect of Trichoderma spp. on tomato is modulated by the plant genotype. Mol Plant Pathol 12:341–354
Vinale F, Marra R, Ruocco M (2014) Trichoderma secondary metabolites active on plants and fungal pathogens. Open Mycol J 8:127–139
Acknowledgements
We thank the Fundacion Produce Chihuahua A.C. for providing financial support for the project (Grant 08-2011-0040). Thanks to the SENASICA-SAGARPA for providing strain Ma70 of Metarhizium anisopliae.
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Guigón López, C., Muñoz Castellanos, L.N., Flores Ortiz, N.A. et al. Control of powdery mildew (Leveillula taurica) using Trichoderma asperellum and Metarhizium anisopliae in different pepper types. BioControl 64, 77–89 (2019). https://doi.org/10.1007/s10526-018-09916-y
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DOI: https://doi.org/10.1007/s10526-018-09916-y