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Elevated extension of longevity by cyclically heat stressing a set of recombinant inbred lines of Drosophila melanogaster throughout their adult life

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Abstract

An extremely high (about 100 %) increase in longevity is reported for a subset of recombinant inbred lines (RILs) of Drosophila melanogaster subjected to a cyclic heat stress throughout the adult life. Previous work showed that both longevity and heat sensitivity highly differed among RILs. The novel heat stress treatment used in this study consisted of 5 min at 38 °C applicated approximately every 125 min throughout the adult life starting at the age of 2 days. In spite of the exceptionally high increase in longevity in a set of RILs, the same heat stress treatment reduced rather than increased longevity in other RILs, suggesting that heat-induced hormesis is dependent on the genotype and/or the genetic background. Further, one quantitative trait locus (QTL) was identified for heat-induced hormesis on chromosome 2 (bands 28A1-34D2) in one RIL panel (RIL-D48) but it was not significant in its reciprocal panel (RIL-SH2). The level of heat-induced hormesis showed a sexual dimorphism, with a higher number of lines exhibiting higher hormesis effects in males than in females. The new heat stress treatment in this study suggests that longevity can be further extended than previously suggested by applying a cyclic and mild stress throughout the life, depending on the genotype.

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Acknowledgments

We thank Volker Loeschcke for earlier collaborations with the setup of RILs. We thank comments from two anonymous reviewers. This research was supported by grants from the University of Buenos Aires, Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), and Consejo nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina) to FMN.

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Authors and Affiliations

  1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires – IEGEBA (CONICET-UBA), C-1428-EHA, Buenos Aires, Argentina

    Federico H. Gomez, Pablo Sambucetti & Fabian M. Norry

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  1. Federico H. Gomez
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  2. Pablo Sambucetti
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  3. Fabian M. Norry
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Correspondence to Federico H. Gomez.

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Gomez, F.H., Sambucetti, P. & Norry, F.M. Elevated extension of longevity by cyclically heat stressing a set of recombinant inbred lines of Drosophila melanogaster throughout their adult life. Biogerontology 17, 883–892 (2016). https://doi.org/10.1007/s10522-016-9658-4

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  • DOI: https://doi.org/10.1007/s10522-016-9658-4

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