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Generation of Monoclonal Antibodies to Recombinant Vascular Endothelial Growth Factor

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF antibodies was performed by ELISA and immunocytochemical analysis on cultured C6 glioma cells. Subsequent cloning yielded hybridoma stably expressing monoclonal anti-VEGF antibodies recognizing recombinant and native VEGF.

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Authors and Affiliations

  1. Department of Medical Nanobiotechnologies, N. I. Pirogov Russian National Research Medical University, Moscow, Russia

    S. A. Shein, A. V. Leopol’d, V. P. Baklaushev, A. A. Korchagina, N. V. Ivanova, N. E. Volgina & V. P. Chekhonin

  2. Department of Fundamental and Applied Neurobiology, V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia

    O. I. Gurina, V. P. Baklaushev, N. F. Grinenko, I. A. Ryabukhin & V. P. Chekhonin

Authors
  1. S. A. Shein
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  2. O. I. Gurina
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  3. A. V. Leopol’d
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  4. V. P. Baklaushev
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  5. A. A. Korchagina
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  6. N. F. Grinenko
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  7. N. V. Ivanova
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  8. N. E. Volgina
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  9. I. A. Ryabukhin
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  10. V. P. Chekhonin
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Corresponding author

Correspondence to S. A. Shein.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 24-28, January, 2012

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Shein, S.A., Gurina, O.I., Leopol’d, A.V. et al. Generation of Monoclonal Antibodies to Recombinant Vascular Endothelial Growth Factor. Bull Exp Biol Med 153, 139–142 (2012). https://doi.org/10.1007/s10517-012-1662-3

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  • DOI: https://doi.org/10.1007/s10517-012-1662-3

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