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Bacillus licheniformis BCR 4-3 increases immune response and survival of Litopenaeus vannamei challenged with Vibrio parahaemolyticus IPNGS16

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Abstract

Immunoprophylactic alternatives are needed to fight against acute hepatopancreatic necrosis disease (AHPND). This study addressed the effects of different routes of Bacillus administration (via diet or culture water) on growth performance, survival, and immune parameters of Litopenaeus vannamei challenged with Vibrio parahaemolyticus-AHPND. Bacillus mixture and Bacillus licheniformis BCR 4-3 were added to feed (1 × 106 CFU g−1) or rearing water (1 × 106, 2 × 106, 3 × 106 CFU L−1). Animals were challenged with a determined median lethal V. parahaemolyticus concentration. Survival, growth performance, and immune response were determined. The results showed that B. licheniformis BCR 4-3 (3 × 106 CFU L−1) administration via culture water significantly (P < 0.05) increased survival (53.4%) in shrimp challenged with V. parahaemolyticus; growth performance was also significantly (P < 0.05) higher compared to the control group. Moreover, the relative expression of innate immune-related genes, such as lysozyme, penaeidin4, crustin, and superoxide dismutase, significantly (P < 0.05) upregulated in shrimp fed B. licheniformis BCR4-3 compared with the control group. In conclusion, the administration of probiotic B. licheniformis BCR4-3 in feed and culture water increased resistance in L. vannamei against V. parahaemolyticus-AHPND and was found associated with an upregulation of key antimicrobial innate immune-related genes.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgments

Dario I. García Medel thanks the Instituto Tecnológico de Boca del Río (16990169) for academic support and D. Fischer for editorial services.

Funding

This study was financed by the Secretaria de Investigacion y Posgrado del Instituto Politecnico Nacional (Mexico) and Consejo Nacional de Ciencia y Tecnologiia (20181360 to A. L-G and PDCPN2014-01/248033 to CA).

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Authors and Affiliations

  1. Tecnológico Nacional de Mexico-Instituto Tecnológico de Boca del Río, Carr. Veracruz-Cordoba Km. 12, Boca del Río, Veracruz, Mexico

    Darío Israel García-Medel

  2. Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, 23096, La Paz, B.C.S., Mexico

    Carlos Angulo

  3. Instituto Politécnico Nacional, Centro Interdiciplinario de Investigación para el Desarrollo Integral Regional (Sinaloa), Blvd. Juan de Dios Bátiz Paredes #250, Guasave, Sinaloa, Mexico

    Ruth Escamilla-Montes, Jesús Arturo Fierro-Coronado, Genaro Diarte-Plata, Carina Gámez-Jiménez & Antonio Luna-González

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  1. Darío Israel García-Medel
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  2. Carlos Angulo
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Highlights

• Bacillus licheniformis BCR 4-3 in water increased survival of shrimp challenged with V. parahaemolyticus-AHPND.

• B. licheniformis BCR 4-3 in diet increased growth performance of shrimp.

• Innate immune-related genes were upregulated in shrimp fed B. licheniformis BCR4-3.

• B. licheniformis BCR4-3 is an immunoprophylactic alternative against AHPND in shrimp Litopenaeus vanammei.

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García-Medel, D.I., Angulo, C., Escamilla-Montes, R. et al. Bacillus licheniformis BCR 4-3 increases immune response and survival of Litopenaeus vannamei challenged with Vibrio parahaemolyticus IPNGS16. Aquacult Int 28, 2303–2318 (2020). https://doi.org/10.1007/s10499-020-00585-2

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