Abstract
Since polyacrylic acid capped platinum nano-particles (nano-Pts) are known to have a unique ability to quench superoxide (O2 −) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome-c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O2 − formation.
Access this article
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Similar content being viewed by others
Abbreviations
- Nano-Pts:
-
Platinum nano particles
- ROS:
-
Reactive oxygen species
- H2O2 :
-
Hydrogen peroxide
- O −2 :
-
Superoxide
- OH:
-
Hydroxyl radical
- OCl− :
-
Hypochlorite
- NO:
-
Nitric oxide
- ONOO:
-
Peroxynitrite
- SOD:
-
Superoxide dismutase
References
Borek C (2004) Antioxidants and Radiation Therapy. J Nutr 134:3207–3209
Riley PA (1994) Free radical in biology: oxidative stress and the effects of ionization radiation. Int J Radiat Biol 65:27–33
Miyata H, Doki Y, Yamamoto H et al (2001) Overexpression of CDC25B overrides radiation-induced G2-M arrest and results in increased apoptosis in esophageal cancer cells. Cancer Res 61:3188–3193
Corbiere C, Liagre B, Terro F, Beneytout JL (2004) Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3 activity in different human cancer cells. Cell Res 14:188–196
Sasano N, Enomoto A, Hosoi Y et al (2007) Free radical scavenger edaravone suppresses X-ray induced apoptosis through p53 inhibition in MOLT-4 cells. J Radiat Res 48:495–503
Tominaga H, Kodama S, Matsuda N, Suzuki K, Watanabe M (2004) Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation. J Radiat Res 45:181–188
Zhang B, Su Y, Ai G, Wang Y, Wang T, Wang F (2005) Involvement of Peroxiredoxin I in protecting cells from radiation-induced death. J Radiat Res 46:305–312
Bhattacharya R, Mukherjee P (2008) Biological properties of ‘‘naked’’ metal nanoparticles. Adv Drug Deliv Rev 60:1289–1306
Kajita M, Hikosaka K, Iitsuka M, Kanayama A, Toshima N, Miyamoto Y (2007) Platinum nanoparticle is a useful scavenger of superoxide anion and hydrogen peroxide. Free Radic Res 41:615–626
Yoshihisa Y, Zhao QL, Hassan MA et al (2010) SOD/catalase mimetic platinum nanoparticles inhibit heat-induced apoptosis in human lymphoma U937 and HH cells. Free Radic Res 45:326–335
Yoshihisa Y, Honda A, Zhao QL et al (2010) Protective effect of platinum nanoparticles against UV-light induced epidermal inflammation. Exp Dermatol 19:1000–1006
Roucoux A, Schulz J, Patin H (2002) Reduced transition metal colloids: a novel family of reusable catalyst? Chem Rev 102:3757–3778
Rehman MU, Yoshihisa Y, Miyamoto Y, Shimizu T (2012) The anti-inflammatory effect of platinum nanoparticles on the lipopolysaccharide induced inflammatory response in RAW 264.7 macrophages. Inflamm Res 61:1177–1185
Kim J, Takahashi M, Shimizu T et al (2008) Effects of potent antioxidant platinum nanoparticle, on life span of Caenorhabditis elegans. Mech Ageing Dev 129:322–331
Sellins KS, Cohen JJ (1987) Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes. J Immunol 139:3199–3206
Royall JA, Ischiropoulos H (1993) Evaluation of 2′,7′-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells. Arch Biochem Biophys 302:348–355
Setsukinai KI, Urano Y, Kakinumas K, Majima HJ, Nagano T (2003) Development of novel fluorescence probes that can reliably detect Reactive oxygen species and distinguish specific species. J Biol Chem 278:3170–3175
Toshima N, Yonezawa T (1998) Bimetallic nanoparticles novel materials for chemical and physical applications. New J Chem 22:1179–1201
Mazza J, Rossi A, Weinberg JM (2010) Innovative uses of tumor necrosis factor alpha inhibitors. Dermatol Clin 28:559–575
Jamieson ER, Lippard SJ (1999) Structure, recognition, and processing of cisplatin–DNA adducts. Chem Rev 99:2467–2498
Asharani PV, Xinyi N, Hande MP, Valiyaveettil S (2010) DNA damage and p53-mediated growth arrest in human cells treated with platinum nanoparticles. Nanomedicine 5:51–64
Zhang X, Zhou X, Chen R, Zhang H (2012) Radiosensitization by inhibiting complex I activity in human hepatoma HepG2 cells to X-ray radiation. J Radiat Res 53:257–263
Hall EJ (2010) The physics and chemistry of radiation absorption. In: Hall EJ (ed) Radiobiology for the radiologist, 7th edn. Lippincott for Williams and Wilkins, New York, pp 1–16
Cui ZG, Kondo T, Feril JLB, Waki K, Inanami O, Kuwabara M (2004) Effects of antioxidants on x-ray or hyperthermia induced apoptosis in human lymphoma U937 cells. Apoptosis 9:757–763
Salganik RI (2001) The benefits and hazards of antioxidants: controlling apoptosis and other protective mechanism in cancer patients and the human population. J Am Coll Nutr 20:464S–475S
Bhuyan BK, Goppi VE (1989) Cell cycle specific inhibitors. Pharm Ther 42:307–348
Yamada T, Ohyama H (1988) Radiation-induced interphase death of rat thymocytes in internally programmed (apoptosis). Int J Radiat Biol 53:65–75
Cui ZG, Kondo T, Ogawa R et al (2004) Enhancement of radiation-induced apoptosis by 6-formylpterin. Free Radic Res 38:363–373
Nitobe J, Yamaguchi S, Okuyama M et al (2003) Reactive oxygen species regulate FLICE inhibitory protein (FLIP) and susceptibility to Fas-mediated apoptosis in cardiac myocytes. Cardiovasc Res 57:119–128
Embree-Ku M, Venturini D, Boekelheide K (2002) Fas is involved in the p53-dependent apoptotic response to ionizing radiation in mouse testis. Biol Reprod 66:1456–1461
Strasser A, Newton K (1999) FADD/MORT1, a signal transducer that can promote cell death or cell growth. Int J Biochem Cell Biol 31:533–537
Yin XM (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res 10:161–167
Wang X (2001) The expanding role of mitochondria in apoptosis. Genes Dev 15:2922–2933
Campion SN, Sandrof MA, Yamasaki H, Boekelheide K (2010) Suppression of radiation-induced testicular germ cell apoptosis by 2,5-hexanedione pretreatment. III. Candidate gene analysis identifies a role for Fas in the attenuation of X-ray–induced apoptosis. Toxicol Sci 117:466–474
Yu DY, Zhao QL, Wei ZL et al (2009) Enhancement of radiation induced apoptosis of human lymphoma U937 cells by sanazole. Apoptosis 14:655–664
Kolesnick R, Fuks Z (2003) Radiation and ceramide-induced apoptosis. Oncogene 22:5897–5906
Nomura M, Yoshimura Y, Kikuiri T et al (2011) Platinum nano particles suppress Osteoclastogenesis through scavenging of reactive oxygen species produced in RAW 264.7 cells. J Pharmacol Sci 117:243–252
Onizawa S, Aoshiba K, Kajita M, Miyamoto Y, Nagai A (2009) Platinum nanoparticle antioxidants inhibit pulmonary inflammation in mice exposed to cigarette smoke. Pulm Pharmacol Ther 22:340–349
Chen M, Wang J (2002) Initiator caspases in apoptosis signaling pathways. Apoptosis 7:313–319
Granville DJ, Gottlieb RA (2002) Mitochondria: regulators of cell death and survival. Sci World J 2:1569–1578
Esposti MD (2002) The roles of Bid. Apoptosis 7:433–440
Li H, Zhu H, Xu CJ, Yuan J (1998) Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 94:491–501
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jawaid, P., Rehman, M.u., Yoshihisa, Y. et al. Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells. Apoptosis 19, 1006–1016 (2014). https://doi.org/10.1007/s10495-014-0972-5
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10495-014-0972-5