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Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan

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Abstract

A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 33 factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found at 30 °C, pH 5, using FT (10 g/l) as substrate. The effect of initial substrate concentration on F (FT0, 5.7–66 g/l) was studied and it was found that F was highest (44.8 U/ml) at FT0 25 g/l. A 22 factorial experimental design with five central points was utilized to study the effect of stirring and aeration on fructanase activity; stirring exhibited a stronger effect on F. The ratio fructanase to invertase (F/S) was 0.57, which confirms that the enzymes are fructanase. Crude fructanase reached high substrate hydrolysis (48 wt%) in 10 h. It is shown that S. cerevisiae CF3 was able to produce large amounts of fructanase by growing it on fructan from A. tequilana.

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Acknowledgments

This work was supported by the Consejo Estatal de Ciencia y Tecnología de Jalisco, México (Project No. PS-2009-765). G. Jacques acknowledges financial support from CONACyT through scholarship No. 234574.

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Authors and Affiliations

  1. Biotechnology Processes Lab, Center of Exact Sciences and Engineering, Department of Chemical Engineering, University of Guadalajara, Blvd. Marcelino García Barragán # 1421, C. P. 44430, Guadalajara, Jalisco, Mexico

    R. I. Corona-González, C. Pelayo-Ortiz, G. Jacques & E. Arriola

  2. Center for Research, Technology Assistance and Design of the State of Jalisco, Av. Normalistas No. 800, Col. Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico

    G. Guatemala

  3. Laboratory of Biotechnology (CUCBA), University of Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales, C.P. 45110, Zapopan, Jalisco, Mexico

    J. A. Arias

  4. Wood, Cellulose and Paper Research Department, University of Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales, C.P. 45110, Zapopan, Jalisco, Mexico

    G. Toriz

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  1. R. I. Corona-González
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  2. C. Pelayo-Ortiz
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  3. G. Jacques
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  4. G. Guatemala
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  5. E. Arriola
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  6. J. A. Arias
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  7. G. Toriz
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Correspondence to C. Pelayo-Ortiz.

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Corona-González, R.I., Pelayo-Ortiz, C., Jacques, G. et al. Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan. Antonie van Leeuwenhoek 107, 251–261 (2015). https://doi.org/10.1007/s10482-014-0323-0

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