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Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E. coli challenged piglets

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Abstract

The effect of feeding Lactobacillus fermentum I5007 on the immune system of weaned pigs with or without E. coli challenge was determined. Twenty-four weaned barrows (6.07 ± 0.63 kg BW) were randomly assigned to one of four treatments (N = 6) in a factorial design experiment. The first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with L. fermentum I5007 (108 CFU/ml) at a daily dose of 20 ml. Pigs in the second two treatments were challenged on the first day with 20 ml of E. coli K88ac (108 CFU/ml). Half of these pigs were not treated while the remaining pigs were treated with 20 ml of L. fermentum I5007 (108 CFU/ml). Peripheral blood lymphocytes subsets were determined using flow cytometry. The intestinal mucosal immunity of the pigs was monitored by real time polymerase chain reaction. The cytokine content of the pig’s serum was also analyzed. Oral administration of L. fermentum I5007 increased blood CD4+ lymphocyte subset percentage as well as tumor necrosis factor-α and interferon-γ expression in the ileum. Pigs challenged with E. coli had elevated jejunal tumor necrosis factor-α while interferon-γ expression was increased throughout the small intestine. There was no difference in the concentration of the cytokines interleukin-2, interleukin-6, tumor necrosis factor-α and interferon-γ in the serum. CD8+ and CD4+/CD8+ in peripheral blood were not affected by treatment. In conclusion, L. fermentum I5007 can enhance T cell differentiation and induce ileum cytokine expression suggesting that this probiotic strain could modulate immune function in piglets.

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Acknowledgments

The investigation was financially supported by the National Basic Research Program (2004 CB 117504) and Beijing Key Technologies of R&D Program Fund.

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Authors and Affiliations

  1. State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, 100193, Beijing, People’s Republic of China

    Aina Wang, Haifeng Yu, Xin Gao, Xiaojie Li & Shiyan Qiao

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  1. Aina Wang
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  2. Haifeng Yu
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  3. Xin Gao
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  4. Xiaojie Li
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  5. Shiyan Qiao
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Correspondence to Shiyan Qiao.

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Wang, A., Yu, H., Gao, X. et al. Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E. coli challenged piglets. Antonie van Leeuwenhoek 96, 89–98 (2009). https://doi.org/10.1007/s10482-009-9339-2

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  • DOI: https://doi.org/10.1007/s10482-009-9339-2

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