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Nutritive utilization of Moringa oleifera tree stalks treated with fungi and yeast to replace clover hay in growing lambs

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Abstract

Twenty four Barki lambs with an average body weight of 20.7 ± 0.17 kg were used in a complete randomized design to evaluate the effects of replacing clover (Trifolium alexandrinum L) hay as a traditional basal diet (C) with moringa tree stalks (MS) treated with fungi (Trichoderma reesei) (MF) and yeast (Saccharomyces cervisiae) (MY) under solid-state fermentation on nitrogen and water metabolism, rumen fermentation and economic efficiency of feeds. Lambs were divided into three groups each with eight lambs depending on their live weight. Concentrate feed mixture was similar for all groups and was offered at 2% of live weight with the basal roughage offered ad libitum. The results show that the rations had significant effects (P < 0.05) on DM, crude protein and nitrogen free extract digestibility. Percentage total digestible nutrients was not affected (P < 0.05) by the experimental rations and the values ranged between 0.62 and 0.64. The MY ration had the highest (P < 0.05) digestible crude protein followed by C and MF. The roughage intake expressed as a percentage of total feed intake for MF and MY groups were higher by 4 and 2%, respectively compared to the control group. Average daily gains were 173, 139 and 146 g/head/day, for C, MF and MY groups, respectively. Average dressing percentages based on either fasting weight or empty weight were not affected by the experimental rations. There were no differences (P < 0.05) among groups in N and water balance. Rumen NH3-N concentrations for the MF and MY groups peaked (P < 0.05) at 3 h post feeding whereas for the control it peaked at 6 h post feeding. Rumen TVFA’s concentrations for all treatments increased gradually from zero to 6 h post-feeding. MY diet recorded the highest (P < 0.05) economical feed efficiency compared with MF and control diet. These results suggest that treatment of moringa stalks with S. cerevisiae for 21 days in a solid-state fermentation system improved its nutritive value and is more suitable for practical feeding in sheep rations.

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Abbreviations

CFM:

Concentrate feed mixture

CW:

Carcass weight

EW:

Empty weight

FCR:

Feed conversion ratio

MS:

Moringa stalks

References

  • Abou-Ammou FF, EI-Badawi AY, Abdel-Khalek TMM, Hafez YH, Mahrous AA, EI-Shafie MH (2011) Nutritional evaluation of a new fungal-yeast probiotic fortified with natural antioxidants on performance of lambs. 2-body weight gain, feed conversion efficiency, carcass characteristics and body composition of two local breeds of sheep raised under two different climatic conditions. Egypt J Nutr Feeds 14:379–391

    Google Scholar 

  • Agosin E, Tollier MT, Brillouet JM, Thivend P, Odier E (1986) Fungal pretreatment of wheat straw. Effect on the biodegradability of cell walls structural polysaccharides, Lignin and phenolic acids by rumen microorganisms. J Sci Food Agric 37:97–106

    Article  CAS  Google Scholar 

  • AOAC (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, DC

    Google Scholar 

  • Aregheore EM (2002) Intake and digestibility of Moringa oleifera and batiki grass mixtures by growing goats. Small Rumin Res 46:23–28

    Article  Google Scholar 

  • Best DA, Lara-Lara PE, Aguilar-Urquizo E, Cen-Chuc FE, Ku-Vera JC, Sangineś-Garciá JR (2017) In vivo digestibility and nitrogen balance in sheep diets with foliage of fodder trees in substitution for soybean meal. Agrofor Syst 91:1079–1085

    Article  Google Scholar 

  • Bhumibhomon O, Chaiyapoland K, Sirisansaneeyakul S (1988) Studies on chemical and environmental changes during composting. Recent advances in biotechnology and applied biology. In: Proceedings of Eighth International Conference on Global Impacts of Applied Microbiology, 1–5 August, 1985, Hong Kong, pp 587–594

  • Cömert M, Şayan Y, Özelçam H, Baykal GY (2015) Effects of Saccharomyces cerevisiae supplementation and anhydrous ammonia treatment of wheat straw on in situ degradability and rumen fermentation and growth performance of yearling lambs. Asian Australas J Anim Sci 28(5):639–646

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Debela E, Tolera A (2013) Nutritive value of botanical fractions of Moringa oleifera and Moringa stenopetala grown in the mid-Rift Valley of southern Ethiopia. Agrofor Syst 87:1147–1155

    Article  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple F test. Biometrics 11:1–42

    Article  Google Scholar 

  • El-Asheeri AK, Hafez YM (2009) Prelimenary indicators of growth, carcass and economic traits of yearling Ossimi and Barki lambs. Egypt J Nutr Feeds 46(1):35–42

    Google Scholar 

  • Elghandour MMY, Vallejo LH, Salem AZM, Mellado M, Camacho LM, Cipriano M, Olafadehan OA, Olivares J, Rojas S (2017) Moringa oleifera leaf meal as an environmental friendly protein source for ruminants: biomethane and carbon dioxide production, and fermentation characteristics. J Clean Prod 165:1229–1238

    Article  CAS  Google Scholar 

  • El-Refaey AM (2006) Effect of biological treatments of some by-products. PhD. Thesis, Faculty of Agricultural Science, Al-Azhar University, Egypt

  • El-Shazly K, Abou-Akkada AR, Naga MN (1963) The use of the in vitro fermentation technique to estimate the digestible energy content of some Egyptian forages. II. The in vitro production of total volatile fatty acids and organic acids as criteria of energy content. J Agric Sci 61:109–114

    Article  Google Scholar 

  • Fadiyimu AA, Julias AA, Fajemisin AN (2010) Digestibility, nitrogen balance and haematological profile of West African Dwarf sheep fed dietary levels of Moringa oliefera as supplement to Panicum maximum. J Anim Sci 6(10):634–643

    Google Scholar 

  • FASS (1999) Guide for the care and use of agricultural animals in agricultural research and teaching. Fed Anim Sci Soc, Champaign

    Google Scholar 

  • Fayed AM, El-Ashry MA, Aziz HA (2009) Effect of feeding Olive tree pruning by-products on sheep performance in Sinai. World J Agric Sci 5(4):436–445

    CAS  Google Scholar 

  • Gebregiorgis F, Negesse T, Nurfeta A (2012) Feed intake and utilization in sheep fed graded levels of dried moringa (Moringa stenopetala) leaf as a supplement to Rhodes grass hay. Trop Anim Health Prod 44(3):511–517

    Article  PubMed  Google Scholar 

  • Graminha EBN, Goncalves AZL, Pirota RD, Balsalobre MAA, DaSilva R, Gomes E (2008) Enzyme production by solid-state fermentation: application to animal nutrition. Anim Feed Sci Technol 144:1–22

    Article  CAS  Google Scholar 

  • Kewan KZ (2013) Impact of total replacement of corn grains with discarded dates in concentrate diets on performance of growing Barki lambs. Egypt J Nutr Feeds 16(2):169–180

    Google Scholar 

  • Kewan KZ, Khattab IM (2016) Livestock and feed resources management in Egypt. LAMBERT Academic Publishing, Germany, p 270

    Google Scholar 

  • Khadem AA, Pahlavan M, Afzalzadeh A, Rezaeinan M (2007) Effects of live yeast Saccharomyces cerevisiae on fermentation parameters and microbial populations of rumen, total tract digestibility of diet nutrients and on the in situ degradability of alfalfa hay in Iranian Chall sheep. Pak J Biol Sci 10:590–597

    Article  CAS  PubMed  Google Scholar 

  • Khalel MS, Shwerab AM, Hassan AA, Yacout MH, El-Badawi AY, Zaki MS (2014) Nutritional evaluation of Moringa oleifera fodder in comparison with trifolium alexandrinum (berseem) and impact of feeding on lactation performance of cows. Life Sci J 11:1040–1054

    Google Scholar 

  • Mahesh MS, Mohini M (2013) Biological treatment of crop residues for ruminant feeding: a review. Afr J Biotechnol 12(27):4221–4231

    Article  CAS  Google Scholar 

  • MALR (2013) Ministry of agriculture and land reclamation. Economic Affairs Sector

  • Marcondes MI, Valadares Filho SC, Detmann E, Valadares RFD, Silva LFC, Fonseca MA (2009) Rumen degradation and intestinal digestibility of crude protein in feeds for cattle. Rev Bras Zootec 38:2247–2257

    Article  Google Scholar 

  • Markham RA (1942) A steam distillation apparatus for micro-Kjeldagl analysis. Biochem J 36:790–791

    CAS  PubMed  PubMed Central  Google Scholar 

  • Merchen NR (1988) Digestion, absorcion y excecion en los rumiantes. In: Church CD (ed) El rumiant, Fiologia digestiva y nutrición. Acribia, Zaragoza, pp 191–223

    Google Scholar 

  • Moyo B, Masika P, Hugo A, Muchenje V (2011) Nutritional characterization of moringa (Moringa oleifera Lam.) leaves. Afr J Biotechnol 60:12925–12933

    Google Scholar 

  • Nsereko VL, Morgavi DP, Furtado AF, Iwausa AD, Wand Y (2002) Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Can J Microbiol 48(1):14–20

    Article  CAS  PubMed  Google Scholar 

  • Omer HAA, Ali FAF, Gad SM (2012) Replacement of clover hay by biologically treated corn stalks in growing sheep rations. J Agric Sci 4(2):257–268

    Google Scholar 

  • Oxoid (1982) Oxoid manual of culture media ingredient and other laboratory surface, 5th edn. Turner graphic LTD, England

    Google Scholar 

  • Permana IG, Termeulen U, Zadrazil F (2004) Cultivation of Pleurotus ostreatus and Lentinus edodes on lignocellulosic substrates for human food and animal feed production. J Agric Rural Dev Trop Suptrop 80:137–143

    Google Scholar 

  • Pridham AP, Foley C, Lindernfelser LA, Hesseltine CW, Benedict RG (1958) A selection of media for maintenance and taxonomic study of Streptomyces. Annual Medical Encyclopedia, Inc., New York, pp 947–953

    Google Scholar 

  • Rossi C, Sgoifo A, Dell-Orto V (2006) Effects of live yeast in beef cattle studied. Feedstuffs 16:11

    Google Scholar 

  • Rufino LDA, Pereira OG, Ribeiro KG, Valadares-Filho SC, Cavali J, Paulino PVR (2013) Effect of substitution of soybean meal for inactive dry yeast on diet digestibility, lamb’s growth and meat quality. Small Rumin Res 111:56–62

    Article  Google Scholar 

  • Sami AS, Shehata MF (2006) Effect of dietary vitamin E supplementation on meat production related traits of Barki lambs. Egypt J Anim Prod 43(1):49–56

    Google Scholar 

  • Sarnklong C, Coneja JW, Pellikaan W, Hendriks W (2010) Utilization of rice straw and different treatments to improve its feed value for ruminants: a review. Asian Autralas J Anim Sci 23:680–692

    Article  Google Scholar 

  • Satter LD, Slyter LL (1974) Effect of ammonia concentration on rumen microbial protein production in vitro. Br J Nutr 32:199–208

    Article  CAS  PubMed  Google Scholar 

  • SPSS (2010) Statistical Package for Social Science, Program Version 19. Chicago, U.S.A

  • Sultana S, Alimon A, Huque KS, Sazili AQ, Yaakub H, Hossain J, Baba M (2015) The Feeding value of moringa (Moringa Oleifera) foliage as replacement to conventional concentrate diet in Bengal goats. Adv Anim Vet Sci 3(3):164–173

    Article  Google Scholar 

  • Thurber MD, Fahey JW (2009) Adoption of Moringa oleifera to combat under-nutrition viewed through the lens of the “Diffusion of Innovations” theory. Ecol Food Nutr 48(3):212–225

    Article  PubMed  PubMed Central  Google Scholar 

  • Tripathi MK, Karim SA (2011) Effect of yeast cultures supplementation on live weight change, rumen fermentation, ciliate protozoa population, microbial hydrolytic enzymes status and slaughtering performance of growing lamb. Livest Sci 135:17–25

    Article  Google Scholar 

  • Umesh-Kumar S, Sudarshan-Singh VK, Kumar U, Singh S (1997) Effect of yeast culture supplement on ruminal microbial population and metabolism in buffalo calves fed a high roughage diet. J Sci Food Agric 73(2):231–236

    Article  Google Scholar 

  • Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597

    Article  PubMed  Google Scholar 

  • Vitti DM, Nozella EF, Abdalla AL, Bueno IC, Silva Filho JC, Costa C, Bueno MS, Longo C, Vieira ME, Cabral Filho SL, Godoy PB, Mueller-Harvey I (2005) The effect of drying and urea treatment on nutritional and anti-nutritional components of browses collected during wet and dry seasons. Anim Feed Sci Technol 122:123–133

    Article  CAS  Google Scholar 

  • Ward JW, Perry TW (1982) Enzymatic conversion of corn cobs to glucose with Trichoderma viride fungus and the effect on nutritional value of the corn cobs. J Anim Sci 54:609–617

    Article  CAS  Google Scholar 

  • Yadov BPS, Yadav IS (1988) Incubation studies with strained rumen liquor of cattle for in vitro evaluation of ammoniated straw. Indian J Anim Sci 58(3):398–402

    Google Scholar 

  • Yu H, Guo G, Zhang X, Yan K, Xu C (2009) The effect of biological pretreatment with the selective white-rot fungus Echinodontium taxodii on enzymatic hydrolysis of softwoods and hardwoods. Bioresour Technol 100:5170–5175

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Animal and Poultry Nutrition Department, Desert Research Center, Mathaf Al-Mattaria, Cairo, 11753, Egypt

    K. Z. Kewan, A. R. Abdou & E. A. Zaki

  2. Animal Production Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra, Cairo, 11241, Egypt

    F. A. Salem & H. M. El-Sayed

  3. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Mexico, Mexico

    A. Z. M. Salem

  4. Agric. Botany Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra, Cairo, 11241, Egypt

    S. S. Eisa

  5. Department of Animal Sciences, School of Agriculture, Pwani University, P.O. Box 195-80108, Kilifi, Kenya

    N. E. Odongo

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  1. K. Z. Kewan
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  2. F. A. Salem
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  3. A. Z. M. Salem
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  4. A. R. Abdou
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  7. E. A. Zaki
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Correspondence to A. Z. M. Salem.

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Kewan, K.Z., Salem, F.A., Salem, A.Z.M. et al. Nutritive utilization of Moringa oleifera tree stalks treated with fungi and yeast to replace clover hay in growing lambs. Agroforest Syst 93, 161–173 (2019). https://doi.org/10.1007/s10457-017-0158-6

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