Abstract
The pervasiveness of pharmaceuticals such as nonsteroidal anti-inflammatory drugs (NSAIDs) in the aquatic ecosystem through the discharge of wastewater, and their potential to biomagnify within this ecosystem, is now recognised. Residues of diclofenac and ibuprofen are currently being detected in surface waters and aquatic organisms throughout the UK and Europe. However, the levels of these residues in fish and other aquatic organisms, particularly lower trophic level prey species, have not yet been determined. While exposure to diclofenac is known to adversely affect fish, the degree to which other aquatic organisms are exposed and impacted through continuous ingestion of contaminated prey and interaction with the aquatic habitat remains unknown. The extent and effects of exposure to ibuprofen also remain largely unknown. As an exploratory subset of a broader study to investigate the detectability of diclofenac in alternative biological matrices, we analysed hair samples from Eurasian otters (Lutra lutra, n = 28) for residues of the two NSAIDs using GC–MS. The otters were collected from six counties in England as part of an ongoing otter health monitoring project at the Wildlife Veterinary Investigation Centre in Chacewater, UK. Diclofenac was qualitatively detected in five hair wash and 15 extract samples, and ibuprofen was determined to be present in at least two of the hair extract samples. Here, we provide preliminary evidence that otters are exposed to both NSAIDs and argue for further studies to identify residue loads in the otters and their prey to fully assess the pervasiveness of these compounds and potential risks of ongoing exposure to them.
References
Ashton D, Hilton M, Thomas KV (2004) Investigating the environmental transport of human pharmaceuticals to streams in the United Kingdom. Sci Total Environ 333:167–184
Baker JR, Jones AM, Jones TP, Watson HC (1981) Otter (Lutra lutra L.) mortality and marine pollution. Biol Conserv 20:311–321
Bendz D, Paxéus NA, Ginn TR, Loge FJ (2005) Occurrence and fate of pharmaceutically active compounds in the environment, a case study: Höje River in Sweden. J Hazard Mater 122:195–204
Cook G (2009) Undergraduate Dissertation, Anglia Ruskin University
D’Havé H, Scheirs J, Mubiana VK, Verhagen R, Blust R, De Coen W (2006) Non-destructive pollution exposure assessment in the European hedgehog (Erinaceus europaeus): II. Hair and spines as indicators of endogenous metal and as concentrations. Environ Pollut (Oxford UK) 142:438–448
Fossi MC, Casini S, Marsili L (1999) Nondestructive biomarkers of exposure to endocrine disrupting chemicals in endangered species of wildlife. Chemosphere 39:1273–1285
Gagné F, Fournier M, Blaise C (2004) Serotogenic effects of municipal effluents: induced spawning activity in freshwater mussels. Fresenius Environ Bull 13:1099–1103
Gagné F, Bérubé E, Fournier M, Blaise C (2005) Inflammatory effects of municipal effluents to Elliptio complanata mussels—lack of effects from anti-inflammatory drugs. Comp Biochem Physiol 141:332–337
Gagné F, Blaise C, André C, Gagnon C, Salazar M (2007) Neuroendocrine disruption and health effects in Ellipto complanata mussels exposed to aeration lagoons for wastewater treatment. Chemosphere 68:731–743
Gόmez MJ, Agüera A, Mezcua M, Hurtado J, Mocholí F, Fernández-Alba AR (2007) Simultaneous analysis of neutral and acidic pharmaceuticals as well as related compounds by gas chromatography–tandem mass spectrometry in wastewater. Talanta 73:314–320
Gonzáles-Barreiro C, Lores M, Casais MC, Cela R (2003) Simultaneous determination of neutral and acidic pharmaceuticals in wastewater by high-performance liquid chromatography–post-column photochemically induced fluorimetry. J Chromatogr A 993:29–37
Hoeger B, Köllner B, Dietrich DR, Hitzfeld B (2005) Water-borne diclofenac affects kidney and gill integrity and selected immune parameters in brown trout (Salmo trutta f. fario). Aquat Toxicol 75:53–64
Jones OAH, Voulvoulis N, Lester JN (2002) Aquatic environmental assessment of the top 25 English prescription pharmaceuticals. Water Res 36:5013–5022
Khetan SK, Collins TJ (2007) Human pharmaceuticals in the aquatic environment: a challenge to green chemistry. Chem Rev 107:2319–2364
Knopp D, Deng A, Letzel M, Taggart M, Himmelsbach M, Zhu QZ, Peröbner I, Kudlak B, Frey S, Sengl M, Buchberger W, Hutchinson C, Cunningham A, Pain D, Cuthbert R, Raab A, Meharg A, Swan G, Jhala Y, Prakash V, Rahmani A, Quevedo M, Niessner R (2008) Immunological determination of the pharmaceutical diclofenac in environmental and biological samples. In: Kennedy IR (ed) Rational environmental management of agrochemicals; risk assessment, monitoring, and remedial action, vol 966. American Chemical Society, Washington, pp 203–226
Koutsouba V, Heberer T, Fuhrmann B, Schmidt-Baumler K, Tsipi D, Hiskia A (2003) Determination of polar pharmaceuticals in sewage water of Greece by gas chromatography–mass spectrometry. Chemosphere 51:69–75
Kruuk H (2006) Otters: ecology, behavior and conservation. Oxford University Press, Oxford
Letzel M, Metzner G, Letzel T (2009) Exposure assessment of the pharmaceutical diclofenac based on long-term measurements of the aquatic input. Environ Int 35:363–368
Mason C (1989) Water pollution and otter distribution: a review. Lutra 32:97–131
Metcalfe CD, Miao XS, Koenig BG, Struger J (2003) Distribution of acidic and neutral drugs in surface waters near sewage treatment plants in the lower great lakes, Canada. Environ Toxicol Chem 22:2881–2889
Meteyer CU, Rideout BA, Gilbert M, Shivaprasad HL, Oaks JL (2005) Pathology and proposed pathophysiology of diclofenac poisoning in free-living and experimentally exposed oriental white-backed vultures (Gyps bengalensis). J Wildl Dis 41:707–716
Nakahara Y (1999) Hair analysis for abused and therapeutic drugs. J Chromatogr B 733:161–180
Oaks JL, Gilbert M, Virani MZ, Watson RT, Meteyer CU, Rideout BA, Shivaprasad HL, Ahmed S, Chaudry MJI, Arshad M, Mahmood S, Ali A, Khan AA (2004) Diclofenac residues as the cause of vulture population decline in Pakistan. Nature 427:630–633
Richards NL (2010) Detection of nonsteroidal anti-inflammatory drugs in hair, nail and feathers using GC–MS, with emphasis on diclofenac: a forensic tool for wildlife conservation. Ph.D. thesis, Anglia Ruskin University
Roberts PH, Thomas KV (2006) The occurrence of selected pharmaceuticals in wastewater effluent and surface waters of the lower Tyne catchment. Sci Total Environ 356:143–153
Schwaiger J, Ferling H, Mallow U, Wintermayr H, Negele RD (2004) Toxic effects of the non-steroidal anti-inflammatory drug diclofenac: Part I: histopathological alterations and bioaccumulation in rainbow trout. Aquat Toxicol 68:141–150
Sebők Á, Vasanits-Szigrai A, Palkó G, Záray G, Molnár-Perl I (2008) Identification and quantification of ibuprofen, naproxen, ketoprofen and diclofenac present in waste-waters, as their trimethylsilyl derivatives, by gas-chromatography mass spectrometry. Talanta 76:642–650
Stülten D, Zühlke S, Lamshöft M, Spiteller M (2008) Occurrence of diclofenac and selected metabolites in sewage effluents. Sci Total Environ 405:310–316
Simpson VR (1997) Health status of otters (Lutra lutra) in south west England based on postmortem findings. Vet Rec 141:191–197
Simpson VR (2007) Health status of otters in southern and south west England 1996–2004. Science Report SCO10064/SR1. Environment Agency, Bristol
Simpson VR, Bain M, Brown R, Brown B, Lacy R (2000) A long-term study of vitamin A and polychlorinated hydrocarbon levels in otters (Lutra lutra) in south west England. Environ Pollut (Oxford, UK) 110:267–275
Smit MD, Leonard PEG, De Jong AWJ, Van Hattum GM (1989) Polychlorinated biphenyls in the Eurasian otter (Lutra lutra). Rev Environ Contam Toxicol 157:95–130
Taastrøm H-M, Jacobsen L (1999) The diet of otters (Lutra lutra L.) in Danish freshwater habitats: comparison of prey fish populations. J Zool 248:1–13
Talmage SS, Walton BT (1991) Small mammals as monitors of environmental contaminants. Rev Environ Contam Toxicol 119:47–145
Ternes TA (1998) Occurrence of drugs in German sewage treatment plants and rivers. Water Res 32:3245–3260
Ternes TA (2001) Analytical methods for the determination of pharmaceuticals in aqueous environmental samples. Trends Anal Chem 20:419–434
Thomas KV, Hilton MJ (2004) The occurrence of selected human pharmaceutical compounds in UK estuaries. Mar Pollut Bull 49:436–444
Triebskorn R, Casper H, Scheil V, Schwaiger J (2007) Ultrastructural effects of pharmaceuticals (carbamazepine, clofibric acid, metoprolol, diclofenac) in rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). Anal Bioanal Chem 387:1405–1416
Verenitch SS, Lowe CJ, Mazumder A (2006) Determination of acidic drugs and caffeine in municipal wastewaters and receiving waters by gas chromatography–ion trap tandem mass spectrometry. J Chromatogr A 1116:193–203
Acknowledgements
Ngaio Richards is indebted to the Foundation for Analytical Science & Technology in Africa (FASTA) for the loan of the Mi-Vac sample condenser and to Dr Richard Shore for facilitating this collaborative work. Vic Simpson would like to thank Fieke Molenaar and Jane Simpson for their assistance with post-mortem examinations, and the Environment Agency for co-ordinating and funding the otter project. Initial drafts of this manuscript were greatly improved by review from Dr Steven Lancaster.
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Richards, N.L., Cook, G., Simpson, V. et al. Qualitative detection of the NSAIDs diclofenac and ibuprofen in the hair of Eurasian otters (Lutra lutra) occupying UK waterways with GC–MS. Eur J Wildl Res 57, 1107–1114 (2011). https://doi.org/10.1007/s10344-011-0513-2
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DOI: https://doi.org/10.1007/s10344-011-0513-2