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Determination of Lipoic Acid in Biological Samples with Acetonitrile–Salt Stacking Method in CE

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Abstract

An acetonitrile–salt stacking method was established for the assay of lipoic acid (LA) in biological samples. Samples were deproteinized with acetonitrile at a final concentration of 60 % (v/v) and then injected hydrodynamically at 3.45 × 103 Pa for 180.0 s. The optimum background electrolyte was found to be 90.0 mmol L−1 pH 9.1 borate buffer. LA could be detected within 35 min at +7.0 kV with satisfactory repeatability (relative standard deviations, RSDs, of migration times and peak areas were both below 10 % for intraday and interday; n = 6/9) and a relatively low limit of detection of ca. 0.5 μmol L−1.

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Acknowledgments

The study was supported by New Century Excellent Talents in University, the Fundamental Research Funds for the Central Universities, National “Twelfth Five-Year” Plan for Science and Technology Support, Shaanxi province science and technology research and development program (2013K12-01-10), and the National Natural Science Foundation of China (Grant No. 31070740, 30930105, 81101674-H1609).

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Authors and Affiliations

  1. Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Frontier Institute of Life Science, FIST, Xi’an Jiaotong University School of Life Science and Technology, Xi’an, 710049, China

    Hua Li, Yu Kong, Liao Chang, Zhihui Feng, Nan Chang, Jiankang Liu & Jiangang Long

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  1. Hua Li
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  2. Yu Kong
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  3. Liao Chang
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  4. Zhihui Feng
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  5. Nan Chang
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  6. Jiankang Liu
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  7. Jiangang Long
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Correspondence to Yu Kong or Jiangang Long.

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Li, H., Kong, Y., Chang, L. et al. Determination of Lipoic Acid in Biological Samples with Acetonitrile–Salt Stacking Method in CE. Chromatographia 77, 145–150 (2014). https://doi.org/10.1007/s10337-013-2560-1

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  • DOI: https://doi.org/10.1007/s10337-013-2560-1

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