Abstract
Object
Examination of blood perfusion in the masseter muscle in the course of repetitive isometric contraction by arterial spin-labeling (ASL) MR imaging and additional T2 relaxation time measurements during and after masseter muscle activation.
Materials and methods
Anatomical and ASL imaging was performed (3 T) in the masseter muscle of seven healthy volunteers before and after sustained clenching (30s) at maximum voluntary contraction (MVC). Several cycles of clenching were repeated in an overall period of 11 min. ASL imaging was performed by an adapted FAIR-TrueFISP technique. Time to peak and time to baseline were systematically analyzed in recorded perfusion curves. T2 relaxation times were estimated using a multi-echo spin-echo sequence. The influence of MVC on T2 was statistically analyzed.
Results
In all cases, perfusion imaging and assessment of T2 relaxation time was feasible. Mean perfusion values at rest calculated from all volunteers were 97.9±17.1ml/min/100 g (right masseter) and 83.0 ± 18.1ml/min/100 g (left masseter). The percentage mean perfusion increase in all volunteers immediately after clenching ranged between 114 and 154%. Mean time to peak was 13.7 s (range: 8.0–26.7 s; SD 5.6 s), and mean time to baseline was 25.6 s (range: 18.7–37.0 s; SD 5.4 s). No significant influence of MVC on T2 relaxation time was found, although a tendency to T2 increase after each clenching stress was observed.
Conclusion
Clear contraction-related perfusion changes of the masseter muscle could be assessed in high spatial and temporal resolution by means of ASL. In contrast, no significant T2 changes were measured. ASL imaging could serve as supplementing tool for studying masticatory function and dysfunction.
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References
Wigmore DM, Damon BM, Pober DM, Kent-Braun JA (2004) MRI measures of perfusion-related changes in human skeletal muscle during progressive contractions. J Appl Physiol 97(6): 2385–2394
Kinugasa R, Kawakami Y, Fukunaga T (2006) Quantitative assessment of skeletal muscle activation using muscle functional MRI. Magn Reson Imaging 24(5): 639–644
Disler DG, Cohen MS, Krebs DE, Roy SH, Rosenthal DI (1995) Dynamic evaluation of exercising leg muscle in healthy subjects with echo planar MR imaging: work rate and total work determine rate of T2 change. J Magn Reson Imaging 5(5): 588–593
Price TB, Kennan RP, Gore JC (1998) Isometric and dynamic exercise studied with echo planar magnetic resonance imaging (MRI). Med Sci Sports Exerc 30(9): 1374–1380
Nygren AT, Kaijser L (2002) Water exchange induced by unilateral exercise in active and inactive skeletal muscles. J Appl Physiol 93(5): 1716–1722
Reid RW, Foley JM, Jayaraman RC, Prior BM, Meyer RA (2001) Effect of aerobic capacity on the T(2) increase in exercised skeletal muscle. J Appl Physiol 90(3): 897–902
Gan Y, Sasai T, Nishiyama H, Ma X, Zhang Z, Fuchihata H (2000) Magnetic resonance imaging of human mandibular elevator muscles after repetitive maximal clenching exercise. Arch Oral Biol 45(3): 247–251
Banci M, Rinaldi E, Ierardi M, Tiberio NS, Boccabella GL, Barbieri C, Scopinaro F, Morelli S, DeSantis M (1998) 99mTc SESTAMIBI scintigraphic evaluation of skeletal muscle disease in patients with systemic sclerosis: diagnostic reliability and comparison with cardiac function and perfusion. Angiology 49(8): 641–648
Kalliokoski KK, Kuusela TA, Nuutila P, Tolvanen T, Oikonen V, Teras M, Takala TE, Knuuti J (2001) Perfusion heterogeneity in human skeletal muscle: fractal analysis of PET data. Eur J Nucl Med 28(4): 450–456
Nakamura Y, Torisu T, Noguchi K, Fujii H (2005) Changes in masseter muscle blood flow during voluntary isometric contraction in humans. J Oral Rehabil 32(8): 545–551
Andersen P, Saltin B (1985) Maximal perfusion of skeletal muscle in man. J Physiol 366: 233–249
de Lussanet QG, van Golde JC, Beets-Tan RG, Post MJ, Huijberts MS, Schaper NC, Kessels AG, van Engelshoven JM, Backes WH (2007) Dynamic contrast-enhanced MRI of muscle perfusion combined with MR angiography of collateral artery growth in a femoral artery ligation model. NMR Biomed 20(8): 25–717
Runge VM (2000) Safety of approved MR contrast media for intravenous injection. J Magn Reson Imaging 12(2): 205–213
Calamante F, Williams SR, van Bruggen N, Kwong KK, Turner R (1996) A model for quantification of perfusion in pulsed labelling techniques. NMR Biomed 9(2): 79–83
Martirosian P, Klose U, Mader I, Schick F (2004) FAIR true-FISP perfusion imaging of the kidneys. Magn Reson Med 51(2): 353–361
Richardson RS, Haseler LJ, Nygren AT, Bluml S, Frank LR (2001) Local perfusion and metabolic demand during exercise: a noninvasive MRI method of assessment. J Appl Physiol 91(4): 1845–1853
Marro KI, Hyyti OM, Vincent MA, Kushmerick MJ (2005) Validation and advantages of FAWSETS perfusion measurements in skeletal muscle. NMR Biomed 18(4): 226–234
Boss A, Martirosian P, Claussen CD, Schick F (2006) Quantitative ASL muscle perfusion imaging using a FAIR-TrueFISP technique at 3.0T. NMR Biomed 19(1): 125–132
Boss A, Martirosian P, Graf H, Claussen CD, Schlemmer HP, Schick F (2005) High resolution MR perfusion imaging of the kidneys at 3 Tesla without administration of contrast media. Rofo 177(12): 1625–1630
Kwong KK, Chesler DA, Weisskoff RM, Donahue KM, Davis TL, Ostergaard L, Campbell TA, Rosen BR (1995) MR perfusion studies with T1-weighted echo planar imaging. Magn Reson Med 34(6): 878–887
Kim SG (1995) Quantification of relative cerebral blood flow change by flow-sensitive alternating inversion recovery (FAIR) technique: application to functional mapping. Magn Reson Med 34(3): 293–301
Deimling M, Heid O (1994) Magnetization prepared true FISP imaging. In: Proceedings of the 2nd annual meeting of the society of magnetic resonance. San Francisco, p 495
Ohlmann B, Rammelsberg P, Henschel V, Kress B, Gabbert O, Schmitter M (2006) Prediction of TMJ arthralgia according to clinical diagnosis and MRI findings. Int J Prosthodont 19(4): 333–338
Zhou J, van Zijl PC (1999) Perfusion imaging using FAIR with a short predelay. Magn Reson Med 41(6): 1099–1107
Ordidge RJ, Wylezinska M, Hugg JW, Butterworth E, Franconi F (1996) Frequency offset corrected inversion (FOCI) pulses for use in localized spectroscopy. Magn Reson Med 36(4): 562–566
Gold GE, Han E, Stainsby J, Wright G, Brittain J, Beaulieu C (2004) Musculoskeletal MRI at 3.0T: relaxation times and image contrast. AJR Am J Roentgenol 183(2): 343–351
Raynaud JS, Duteil S, Vaughan JT, Hennel F, Wary C, Leroy-Willig A, Carlier PG (2001) Determination of skeletal muscle perfusion using arterial spin labeling NMRI: validation by comparison with venous occlusion plethysmography. Magn Reson Med 46(2): 305–311
MacFall JR, Wehrli FW, Breger RK, Johnson GA (1987) Methodology for the measurement and analysis of relaxation times in proton imaging. Magn Reson Imaging 5(3): 209–220
Delcanho RE, Kim YJ, Clark GT (1996) Haemodynamic changes induced by submaximal isometric contraction in painful and non-painful human masseter using near-infra-red spectroscopy. Arch Oral Biol 41(6): 585–596
Larsson R, Oberg PA, Larsson SE (1999) Changes of trapezius muscle blood flow and electromyography in chronic neck pain due to trapezius myalgia. Pain 79(1): 45–50
Pell GS, King MD, Proctor E, Thomas DL, Lythgoe MF, Gadian DG, Ordidge RJ (2003) Comparative study of the FAIR technique of perfusion quantification with the hydrogen clearance method. J Cereb Blood Flow Metab 23(6): 689–699
Frank LR, Wong EC, Haseler LJ, Buxton RB (1999) Dynamic imaging of perfusion in human skeletal muscle during exercise with arterial spin labeling. Magn Reson Med 42(2): 258–267
Richardson RS, Haseler LJ, Nygren AT, Bluml S, Frank LR (2001) Local perfusion and metabolic demand during exercise: a noninvasive MRI method of assessment. J Appl Physiol 91(4): 1845–1853
Van Leeuwen BE, Barendsen GJ, Lubbers J, de Pater L (1992) Calf blood flow and posture: Doppler ultrasound measurements during and after exercise. J Appl Physiol 72(5): 1675–1680
Murrant CL, Sarelius IH (2000) Coupling of muscle metabolism and muscle blood flow in capillary units during contraction. Acta Physiol Scand 168(4): 531–541
Thompson BC, Fadia T, Pincivero DM, Scheuermann BW (2007) Forearm blood flow responses to fatiguing isometric contractions in women and men. Am J Physiol Heart Circ Physiol 293(1): 805–812
Korfage JA, Brugman P, Van Eijden TM (2000) Intermuscular and intramuscular differences in myosin heavy chain composition of the human masticatory muscles. J Neurol Sci 178(2): 95–106
Osada T, Katsumura T, Murase N, Sako T, Higuchi H, Kime R, Hamaoka T, Shimomitsu T (2003) Post-exercise hyperemia after ischemic and non-ischemic isometric handgrip exercise. J Physiol Anthropol Appl Hum Sci 22(6): 299–309
Kim YJ, Kuboki T, Tsukiyama Y, Koyano K, Clark GT (1999) Haemodynamic changes in human masseter and temporalis muscles induced by different levels of isometric contraction. Arch Oral Biol 44(8): 641–650
Varela JM, Castro NB, Biedma BM, Da Silva Dominguez JL, Quintanilla JS, Munoz FM, Penin US, Bahillo JG (2003) A comparison of the methods used to determine chewing preference. J Oral Rehabil 30(10): 990–994
Damon BM, Gregory CD, Hall KL, Stark HJ, Gulani V, Dawson MJ (2002) Intracellular acidification and volume increases explain R(2) decreases in exercising muscle. Magn Reson Med 47(1): 14–23
Chikui T, Shiraishi T, Tokumori K, Inatomi D, Hatakenaka M, Yuasa K, Yoshiura K (2010) Assessment of the sequential change of the masseter muscle by clenching: a quantitative analysis of T1, T2, and the signal intensity of the balanced steady-state free precession. Acta Radiol 51(6): 669–678
Kinugasa R, Kawakami Y, Fukunaga T (2006) Mapping activation levels of skeletal muscle in healthy volunteers: an MRI study. J Magn Reson Imaging 24(6): 1420–1425
Wu WC, Wang J, Detre JA, Ratcliffe SJ, Floyd TF (2008) Transit delay and flow quantification in muscle with continuous arterial spin labeling perfusion-MRI. J Magn Reson Imaging 28(2): 445–452
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C. Schraml and N. F. Schwenzer contributed equally.
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Schraml, C., Schwenzer, N.F., Martirosian, P. et al. Temporal course of perfusion in human masseter muscle during isometric contraction assessed by arterial spin labeling at 3T. Magn Reson Mater Phy 24, 201–209 (2011). https://doi.org/10.1007/s10334-011-0254-y
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DOI: https://doi.org/10.1007/s10334-011-0254-y