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A pqr2 mutant encodes a defective polyamine transporter and is negatively affected by ABA for paraquat resistance in Arabidopsis thaliana

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Abstract

Despite the paraquat-resistant mutants that have been reported in plants, this study identified a novel A. thaliana mutant (pqr2) from an XVE inducible activation library based on its resistance to 2 μM paraquat. The pqr2 mutant exhibited a termination mutation in the exon of AT1G31830/PAR1/PQR2, encoded a polyamine uptake transporter AtPUT2/PAR1/PQR2. The PQR2 mutation could largely reduce superoxide accumulation and cell death in the pqr2 plants under paraquat treatment. Moreover, compared with wild type, the pqr2 mutant exhibited much reduced tolerance to putrescine, a classic polyamine compound, which confirmed that PQR2 encoded a defective polyamine transporter. Notably, co-treated with ABA and paraquat, both pqr2 mutant and wild type exhibited a lethal phenotype from seed germination, but the wild type like pqr2 mutant, could remain paraquat-resistance while co-treated with high dosage of Na2WO4, an ABA synthesis inhibitor. Gene expression analysis suggested that ABA signaling should widely regulate paraquat-responsive genes distinctively in wild type and pqr2 mutant. Hence, this study has for the first time reported about ABA negative effect on paraquat-resistance in A. thaliana, providing insight into the ABA signaling involved in the oxidative stress responses induced by paraquat in plants.

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Acknowledgments

This work was supported by grants from the 111 Project (B08032), and the Fundamental Research Funds for the Central Universities (2013QC042). We thank the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences for kindly providing the XVE inducible activation lines.

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Authors and Affiliations

  1. Biomass and Bioenergy Research Centre, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Shuchao Dong, Huizhen Hu, Youmei Wang, Zhengdan Xu, Yi Zha, Xiwen Cai, Liangcai Peng & Shengqiu Feng

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Shuchao Dong, Huizhen Hu, Youmei Wang, Zhengdan Xu, Yi Zha & Shengqiu Feng

  3. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58102, USA

    Xiwen Cai

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  1. Shuchao Dong
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Correspondence to Shengqiu Feng.

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Dong, S., Hu, H., Wang, Y. et al. A pqr2 mutant encodes a defective polyamine transporter and is negatively affected by ABA for paraquat resistance in Arabidopsis thaliana . J Plant Res 129, 899–907 (2016). https://doi.org/10.1007/s10265-016-0819-y

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