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The Omp85-type outer membrane protein p36 of Arabidopsis thaliana evolved by recent gene duplication

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Abstract

Proteins of the Omp85 family are involved in the insertion of β-barrel shaped outer membrane proteins in bacteria and mitochondria, and—at least—in the transfer of preproteins across the chloroplast outer envelope. In general these proteins consist of up to five N-terminal “polypeptide transport associated” (POTRA) domains and a C-terminal, membrane embedded β-barrel domain. In Arabidopsis thaliana two plastidic gene families coding for Omp85-like proteins exist, namely the Toc75-III and the Toc75-V/Oep80 sub-family. The latter is composed of three genes, of which two do not contain POTRA domains. These are annotated as P39 and P36. However, P36 resulted from a very recent gene duplication of P39 and appears to be specific to Arabidopsis thaliana. Furthermore, we show that P39 is specifically expressed in vein tissues, while P36 is expressed at early and late developmental stages. T-DNA insertion in P36 causes a mild phenotype with reduced starch accumulation in chloroplasts of sepals pointing towards a yet to be described plastid function.

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Abbreviations

TOC:

Translocon on the outer envelope of chloroplasts

Toc75:

Translocon on the outer envelope of chloroplasts subunit of 75 kDa

OEP80:

Outer envelope protein of 80 kDa

Omp85:

Outer membrane protein of 85 kDa

POTRA:

Polypeptide transport associated domain

PTB:

Polypeptide-transporting β-barrel channels

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Acknowledgments

We thank Maike Ruprecht, Daniela Bublak and Gisela Englich for technical assistance. Further we thank Alfred Batschauer, Marburg for providing us with GV3191 and pMP90. This work was supported by grants from the Deutsche Forschungsgemeinschaft, CEF “Macromolecular Complexes” and SFB807/P17 to ES. Support from EMBO (short term fellowship, KN), and IMPRES Frankfurt (fellowship, YCH) is acknowledged.

Author information

Author notes

  1. Sandra Missbach

    Present address: Novartis Vaccines and Diagnostics GmbH, Emil-von-Behring-Str. 76, 35041, Marburg, Germany

  2. Franziska Ertel

    Present address: Department of Biochemistry, McGill University Montreal, Montreal, H3G 1Y6, Canada

Authors and Affiliations

  1. Department of Molecular Cell Biology of Plants, Goethe University, Max-von-Laue Str. 9, 60438, Frankfurt, Germany

    Kerstin Nicolaisen, Sandra Missbach, Yi-Ching Hsueh, Franziska Ertel, Maik S. Sommer & Enrico Schleiff

  2. Center of Membrane Proteomics, Goethe University, Max-von-Laue Str. 9, 60438, Frankfurt, Germany

    Kerstin Nicolaisen & Enrico Schleiff

  3. Cluster of Excellence Frankfurt, Goethe University, 60438, Frankfurt, Germany

    Enrico Schleiff

  4. Buchman Institute of Molecular Life Sciences, Goethe University, Max-von-Laue Str. 15, 60438, Frankfurt, Germany

    Enrico Schleiff

  5. Institute Ruđer Bošković, Zavod za molekularnu biology, 10 000, Zagreb, Croatia

    Hrvoje Fulgosi

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  1. Kerstin Nicolaisen
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Correspondence to Enrico Schleiff.

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Nicolaisen, K., Missbach, S., Hsueh, YC. et al. The Omp85-type outer membrane protein p36 of Arabidopsis thaliana evolved by recent gene duplication. J Plant Res 128, 317–325 (2015). https://doi.org/10.1007/s10265-014-0693-4

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