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Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsis thaliana, with their interacting partners

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Abstract

The light, oxygen, or voltage (LOV) domain that belongs to the Per-ARNT-Sim (PAS) domain superfamily is a blue light sensory module. The Arabidopsis thaliana PAS/LOV PROTEIN (PLP) gene encodes three putative blue light receptor proteins, PLPA, PLPB, and PLPC, because of its mRNA splicing variation. PLPA and PLPB each contain one PAS domain at the N-terminal region and one LOV domain at the C-terminal region, while the LOV domain is truncated in PLPC. RNA gel blot analysis showed that PLP mRNA was markedly expressed after exposure to salt or dehydration stress. Yeast two-hybrid screening led to the isolation of VITAMIN C DEFECTIVE 2 (VTC2), VTC2-LIKE (VTC2L), and BEL1-LIKE HOMEODOMAIN 10 proteins (BLH10A and BLH10B) as PLP-interacting proteins. The molecular interaction of PLPA with VTC2L, BLH10A or BLH10B, and that of PLPB with VTC2L were diminished when yeasts were grown under blue light illumination. Furthermore, the possible binding of flavin chromophore to PLPA and PLPB was demonstrated. These results imply that the LOV domain of PLPA and PLPB functions as a blue light sensor, and suggest the applicability of these interactions to blue light-dependent switching in transcriptional regulation in yeast or other organisms.

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Acknowledgments

We thank RIKEN BRC (Tsukuba, Japan) for providing full-length cDNAs for PLPB, VTC2, VTC2L, and BLH10A, and ABRC (Columbus, OH, USA) for providing T20F6 BAC clone and cDNA libraries. We thank Kayo Sato, Emi Takata, and Noriko Uemura for technical assistance, and Yoko Tokioka and Kei Hirano for initial work. This work was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 17084008 to ST, No. 17084006 to MW, and No. 17084003 to TK).

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Authors and Affiliations

  1. Division of Genome Analysis and Genetic Research, Life Science Research Center, Institute of Research Promotion, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Yasunobu Ogura & Tomohiro Kiyosue

  2. Department of Life Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Akihiro Komatsu & Tomohiro Kiyosue

  3. Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka, 599-8531, Japan

    Kazunori Zikihara & Satoru Tokutomi

  4. Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

    Tokihiko Nanjo

  5. Division of Photobiology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan

    Masamitsu Wada

Authors
  1. Yasunobu Ogura
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  2. Akihiro Komatsu
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  3. Kazunori Zikihara
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  4. Tokihiko Nanjo
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  5. Satoru Tokutomi
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  6. Masamitsu Wada
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  7. Tomohiro Kiyosue
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Corresponding author

Correspondence to Tomohiro Kiyosue.

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Yasunobu Ogura and Akihiro Komatsu contributed equally to this work.

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Ogura, Y., Komatsu, A., Zikihara, K. et al. Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsis thaliana, with their interacting partners. J Plant Res 121, 97–105 (2008). https://doi.org/10.1007/s10265-007-0118-8

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  • DOI: https://doi.org/10.1007/s10265-007-0118-8

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