Abstract
We have cloned two genes for gibberellin (GA) 2-oxidase from rice (Oryza sativa L.). Expression of OsGA2ox2 was not observed. The other gene, OsGA2ox3, was expressed in every tissue examined and was enhanced by the application of biologically active GA. Recombinant OsGA2ox3 protein catalyzed the metabolism of GA1 to GA8 and GA20 to GA29-catabolite. These results indicate that OsGA2ox3 is involved in the homeostatic regulation of the endogenous level of biologically active GA in rice.
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Acknowledgements
This work was supported in part by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences (to H.T. and M.M.).
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M. Sakai and T. Sakamoto contributed equally to this work.
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Sakai, M., Sakamoto, T., Saito, T. et al. Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins. J Plant Res 116, 161–164 (2003). https://doi.org/10.1007/s10265-003-0080-z
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DOI: https://doi.org/10.1007/s10265-003-0080-z