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Ectopic expression of CSD1 and CSD2 targeting genes of miR398 in grapevine is associated with oxidative stress tolerance

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Abstract

MicroRNAs (miRNAs) are endogenous small RNAs of −21 nucleotides that play an important role in diverse plant physiological processes at the post-transcriptional level by directing mRNA cleavage or translational inhibition. Previous studies have indicated that down-regulation of miR398 in response to oxidative stress allows up-regulation of the two target genes, cytosolic CSD1 and chloroplastic CSD2 (copper/zinc superoxide dismutase), resulting in protecting the plants to tolerate oxidative stress. In this study, we provide evidence that grapevine miR398 (Vv-miR398), by regulating the expression of its target genes, VvCSD1 and VvCSD2, mediates responses of grapevine to copper (Cu) stress which have been magnified due to increase in Cu-containing pesticide application. The expression of Vv-miR398 was inhibited by different concentrations of Cu stress; on the other hand, there was a steady increase in the activity of VvCSD1 and VvCSD2 genes. The function of VvCSD1 and VvCSD2 under Cu stress was thoroughly examined by overexpressing the use of the VvCSD1 and VvCSD2 in transgenic tobacco (Nicotiana tabacum). We found that both the overexpressed transgenic lines had lower Cu sensitivity and higher Cu tolerance compared with the wild type. In addition, lower levels of ROS and higher levels of SOD activities were accumulated in the transgenic lines in comparison with the wild type under the higher Cu conditions. Furthermore, these transgenic tobacco lines also recorded a higher UV and salt tolerance than the WT plants. These results suggested that overexpressing the VvCSDs will enhance the ROS-scavenging systems and protect the plant against more oxidative damage. Also, more investigations in this line are needed that would provide significant improvements in our understanding the resistance of fruit crops to environmental stress.

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Acknowledgments

This work was supported by grants from China Postdoctoral Science Foundation (2015M581811), the Natural Science Foundation of China (NSFC) (No. 31401846), the Fundamental Research Funds for the Central Universities (KJQN201540), the Postdoc Foundation of China (2014M561664), the Postdoc Foundation of Jiangsu Province (1401051B).

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  1. XiangPeng Leng and Peipei Wang contributed equally to this work.

Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    XiangPeng Leng, Xudong Zhu, Xiaopeng Li, Ting Zheng, Lingfei Shangguan & Jinggui Fang

  2. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Peipei Wang

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  1. XiangPeng Leng
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Correspondence to Lingfei Shangguan or Jinggui Fang.

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Leng, X., Wang, P., Zhu, X. et al. Ectopic expression of CSD1 and CSD2 targeting genes of miR398 in grapevine is associated with oxidative stress tolerance. Funct Integr Genomics 17, 697–710 (2017). https://doi.org/10.1007/s10142-017-0565-9

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