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188Re-labeled hyperbranched polysulfonamine as a robust tool for targeted cancer diagnosis and radioimmunotherapy

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Abstract

Hyperbranched polysulfonamine (HPSA) is a promising biomaterial due to its highly branched spherical architecture and efficient intracellular translocation. To realize the functionalization of HPSA, both N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) for tethering the human-mouse chimeric monoclonal antibody CH12 and N-hydroxy succinimidyl S-acetylmercaptoacetyltriglycinate (NHS-MAG3) for labeling 188Re were sequentially grafted onto the primary amine terminals of HPSA via covalent linkages, attaining the SPDP-HPSA-MAG3 intermediate. In order to reserve the structural integrity of CH12, the fragment crystallizable (Fc) region was also processed by oxidation of oligosaccharide moieties with sodium periodate and then reacted with N-(κ-maleimidoundecanoic acid) hydrazide (KMUH). After chelating 188Re with MAG3 group, the SPDP was reduced to PDP and connected onto the maleinimide group at the Fc region. As a result, both the epidermal growth factor receptor vIII (EGFRvIII) targeted monoclonal antibody CH12 and the radionuclide 188Re were conjugated to the HPSA-based vehicles, forming the 188Re-labeled and CH12-tethered HPSA (CH12-HPSA-188Re). The molecular weight and in vitro stability of CH12-HPSA-188Re were evaluated by gel electrophoresis and paper chromatography. On one hand, the CH12-HPSA-188Re could specifically bind to the EGFRvIII-positive human hepatocarcinoma cells in vitro. On the other hand, it could also target at the tumor tissue of nude mice in vivo. Hence, the CH12-HPSA-188Re could effectively target at the human hepatocarcinoma and facilitate the tumor detection and targeted radioimmunotherapy.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China

    Nan Li, Li-zhe Xue & Pei-yong Li  (李培勇)

  2. School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 200240, China

    Yue Jin, De-yue Yan & Xin-yuan Zhu  (朱新远)

Authors
  1. Nan Li
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  2. Yue Jin
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  3. Li-zhe Xue
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  4. Pei-yong Li  (李培勇)
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  5. De-yue Yan
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  6. Xin-yuan Zhu  (朱新远)
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Corresponding authors

Correspondence to Pei-yong Li  (李培勇) or Xin-yuan Zhu  (朱新远).

Additional information

The work was financially supported by the National Natural Science Foundation of China (Nos. 20974062, 30700175), National Basic Research Program (Nos. 2009CB930400, 2012CB821500), and China National Funds for Distinguished Young Scientists (No. 21025417).

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Li, N., Jin, Y., Xue, Lz. et al. 188Re-labeled hyperbranched polysulfonamine as a robust tool for targeted cancer diagnosis and radioimmunotherapy. Chin J Polym Sci 31, 530–540 (2013). https://doi.org/10.1007/s10118-013-1242-7

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  • DOI: https://doi.org/10.1007/s10118-013-1242-7

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