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Quantitative indirect ELISA for determination of walnut proteins in foods

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Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein. Epitopes of walnut proteins had no cross activity. The indirect ELISA was highly specific for soluble walnut proteins. Recovery values from walnut protein solutions ranged from 88.47 to 115%, and low coefficients of variation (<10%) indicated good repeatability. Intra and inter-assay precisions were <6 and <7 %, respectively. The limit of detection was 10 ng/mL of soluble walnut protein. The method was applied to detect some commercial food products in China and the results showed that indirect ELISA could be promisingly used to quantify adulteration of walnut processed foods.

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China

    Juan Fang, Chaoyin Chen, Feng Ge, Diqiu Liu, Benyong Han & Xiangfeng Xiong

  2. Yunnan Tobacco Quality Supervision and Inspection Station, Kunming, 650106, China

    Dan Chen

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  1. Juan Fang
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  2. Dan Chen
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  3. Chaoyin Chen
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  4. Feng Ge
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  6. Benyong Han
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  7. Xiangfeng Xiong
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Correspondence to Chaoyin Chen.

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Fang, J., Chen, D., Chen, C. et al. Quantitative indirect ELISA for determination of walnut proteins in foods. Food Sci Biotechnol 24, 2251–2255 (2015). https://doi.org/10.1007/s10068-015-0300-z

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  • DOI: https://doi.org/10.1007/s10068-015-0300-z

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