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Galectin-3 gene (LGALS3) +292C allele is a genetic predisposition factor for rheumatoid arthritis in Taiwan

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Abstract

Galectin-3 is a beta-galactoside-binding lectin which is involved in modulating inflammation and apoptosis. Elevated expression of galectin-3 has been demonstrated in synovium of rheumatoid arthritis (RA). The aim of our study is to investigate the genetic polymorphisms of galectin-3 in association with RA. Polymorphisms of galectin-3 gene (LGALS3) were compared between 151 RA patients and 182 healthy subjects in Taiwan. Variants at two LGALS3 single nucleotide polymorphism (SNP) sites (rs4644 and rs4652, corresponding to LAGLS3 +191 and +292) were genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and sequence-specific oligonucleotide probe hybridization, respectively. The allelic carriage of LGALS3 +292C was increased in patients with RA (66.9% in RA vs. 52.7% in controls, odds ratio = 1.8, 95% confidence interval = 1.2–2.8, p = 0.009). These results implicate that the genetic polymorphisms in galectin-3 gene may contribute to development of RA.

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Acknowledgments

This study was supported by grants from the National Science Council (NSC-94-2320-B-002-085), National Taiwan University Hospital (VN 9801), and National Health Research Institute (NHRI-EX95-9532SI), Taiwan. Polyclonal galectin-3 antibodies used in detecting serum galectin-3 were kindly provided by Dr. Liu FT.

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Authors and Affiliations

  1. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China

    Chung-Yi Hu & Sheng-Kai Chang

  2. Department of Internal Medicine, Far Eastern Memorial Hospital, Taipei, Taiwan, Republic of China

    Chien-Sheng Wu

  3. Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, Republic of China

    Chien-Sheng Wu, Wei-I Tsai & Ping-Ning Hsu

  4. Graduate Institute of Immunology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, Taiwan, Republic of China

    Ping-Ning Hsu

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  1. Chung-Yi Hu
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Correspondence to Ping-Ning Hsu.

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Hu, CY., Chang, SK., Wu, CS. et al. Galectin-3 gene (LGALS3) +292C allele is a genetic predisposition factor for rheumatoid arthritis in Taiwan. Clin Rheumatol 30, 1227–1233 (2011). https://doi.org/10.1007/s10067-011-1741-2

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  • DOI: https://doi.org/10.1007/s10067-011-1741-2

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