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Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity

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Abstract

With the development of nanotechnology, nanoscale products that are smaller than several hundred nanometers have been applied to all areas of science and technology. Nanoscale products, including carbon nanotubes, fullerene derivatives, and nanocrystal quantum dots (QDs), are wide spread as novel tools in various fields, not only in materials engineering, electronics, plastics, and the automobile and aerospace industries, but also in molecular biology and medicine. At present, QDs have been widely used in biological and medical studies because of their superior photoemission and photostability. Although the physical and chemical properties of QDs have been circumstantially investigated, little is known about any harmful effects of QDs on human health. Here we report on the toxicity and biological behavior of QDs in vitro and in vivo. The toxicity of the core constituent chemicals such as cadmium and selenium has been identified. Recently, the surface molecules surrounding QDs have been intensively investigated. Accumulating evidence that toxic surface-covering molecules showed their cytotoxicity and biomolecules conjugated with QDs maintained their biological effects indicates that at least the biological properties of QDs are attributable to the QD-capping material rather than to the core metalloid complex itself.

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Authors and Affiliations

  1. International Clinical Research Center, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo, 162-8655, Japan

    Akiyoshi Hoshino Ph.D, Noriyoshi Manabe, Kouki Fujioka Ph.D & Kenji Yamamoto MD, Ph.D

  2. Department of Pharmacokinetics and Pharmacodynamics, Hospital Pharmacy, Tokyo Medical and Dental University Graduate School of Medicine, Tokyo, Japan

    Akiyoshi Hoshino Ph.D, Masato Yasuhara Ph.D & Kenji Yamamoto MD, Ph.D

  3. Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan

    Akiyoshi Hoshino Ph.D & Kazuo Suzuki Ph.D

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  1. Akiyoshi Hoshino Ph.D
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  2. Noriyoshi Manabe
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  3. Kouki Fujioka Ph.D
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  4. Kazuo Suzuki Ph.D
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  5. Masato Yasuhara Ph.D
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  6. Kenji Yamamoto MD, Ph.D
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Correspondence to Kenji Yamamoto MD, Ph.D.

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Hoshino, A., Manabe, N., Fujioka, K. et al. Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity. J Artif Organs 10, 149–157 (2007). https://doi.org/10.1007/s10047-007-0379-y

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