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Electrochemical glucose biosensor based on silver nanoparticles/multiwalled carbon nanotubes modified electrode

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Abstract

A novel glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) on Ag nanoparticles-decorated multiwalled carbon nanotube (AgNP-MWNT) modified glass carbon electrode (GCE). The AgNP-MWNT composite membrane showed an improving biocompatibility for GOx immobilization and an enhancing electrocatalytic activity toward reduction of oxygen due to decoration of AgNPs on MWNT surfaces. The AgNPs also accelerated the direct electron transfer between redox-active site of GOx and GCE surface because of their excellent conductivity and large capacity for protein loading, leading to direct electrochemistry of GOx. The glucose biosensor of this work showed a lower limit of detection of 0.01 mM (S/N = 3) and a wide linear range from 0.025 to 1.0 mM, indicating an excellent analytical performance of the obtained biosensor to glucose detection. The resulting biosensor exhibits good stability and excellent reproducibility. Such bionanocomposite provides us good candidate material for fabrication of biosensors based on direct electrochemistry of immobilized enzymes.

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References

  1. Wang SG, Zhang Q, Wang RL, Yoon SF (2003) A novel multi-walled carbon nanotube-based biosensor for glucose detection. Biochem Biophys Res Commun 311:572–576

    Article  CAS  Google Scholar 

  2. Valcárcel M, Cárdenas S, Simonet BM (2007) Role of carbon nanotubes in analytical science. Anal Chem 79:4788–4797

    Article  Google Scholar 

  3. Wang J, Dai JH, Yarlagadda T (2005) Carbon nanotube-conducting-polymer composite nanowires. Langmuir 21:9–12

    Article  Google Scholar 

  4. Wang T, Hu XG, Dong SJ (2007) Construction of metal nanoparticle/multiwalled carbon nanotube hybrid nanostructures providing the most accessible reaction sites. J Mater Chem 17:4189–4195

    Article  CAS  Google Scholar 

  5. Zou YJ, Xiang CL, Sun LX, Xu F (2008) Glucose biosensor based on electrodeposition of platinum nanoparticles onto carbon nanotubes and immobilizing enzyme with chitosan-SiO2 sol-gel. Biosens Bioelectron 23:1010–1016

    Article  CAS  Google Scholar 

  6. Zhang MG, Gorski W (2005) Electrochemical sensing platform based on the carbon nanotubes/redox mediators-biopolymer system. J Am Chem Soc 127:2058–2059

    Article  CAS  Google Scholar 

  7. Chu X, Duan DX, Shen GL, Yu RQ (2007) Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode. Talanta 71:2040–2047

    Article  CAS  Google Scholar 

  8. Yang MH, Yang YH, Liu YL, Shen GL, Yu RQ (2006) Platinum nanoparticles-doped sol–gel/carbon nanotubes composite electrochemical sensors and biosensors. Biosens Bioelectron 21:1125–1131

    Article  CAS  Google Scholar 

  9. Jia JB, Wang BQ, Wu AG, Cheng GJ, Li Z, Dong SJ (2002) A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network. Anal Chem 74:2217–2223

    Article  CAS  Google Scholar 

  10. Patolsky F, Gabriel T, Willner I (1999) Controlled electrocatalysis by microperoxidase-11 and Au-nanoparticle superstructures on conductive supports. J Electroanal Chem 479:69–73

    Article  CAS  Google Scholar 

  11. Li FH, Wang ZH, Shan CS, Song JF, Han DX, Niu L (2009) Preparation of gold nanoparticles/functionalized multiwalled carbon nanotube nanocomposites and its glucose biosensing application. Biosens Bioelectron 24:1765–1770

    Article  CAS  Google Scholar 

  12. Xu H, Zeng LP, Xing SJ, Shi GY, Xian YZ, Jin L (2008) Microwave-radiated synthesis of gold nanoparticles/carbon nanotubes composites and its application to voltammetric detection of trace mercury (II). Electrochem Commun 10:1839–1843

    Article  CAS  Google Scholar 

  13. Zhang YZ, Zhang KY, Ma HY (2009) Electrochemical DNA biosensor based on silver nanoparticles/poly(3-(3-pyridyl)acrylic acid)/carbon nanotubes modified electrode. Anal Biochem 387:13–19

    Article  CAS  Google Scholar 

  14. Yang ZY, Li JP, Fang C (2005) A biosensor based on the glucose oxidase film modified by titanic sol–gel doping with Prussian Blue nanoparticles. Chin J Anal Chem 33:538–542

    CAS  Google Scholar 

  15. Yang YH, Yang GM, Huang Y, Bai HP, Lu XX (2009) A new hydrogen peroxide biosensor based on gold nanoelectrode ensembles/multiwalled carbon nanotubes/chitosan film-modified electrode. Colloids Surf A Physicochem Eng Asp 340:50–55

    Article  CAS  Google Scholar 

  16. Tsai MC, Tsai YC (2009) Adsorption of glucose oxidase at platinum-multiwalled carbon nanotube-alumina-coated silica nanocomposite for amperometric glucose biosensor. Sensors Actuators B 141:592–598

    Article  Google Scholar 

  17. Zhang J, Gao L (2010) Synthesis of highly dispersed platinum nanoparticles on multiwalled carbon nanotubes and their electrocatalytic activity toward hydrogen peroxide. J Alloy Comp 505:604–608

    Article  CAS  Google Scholar 

  18. Qin X, Wang HC, Wang XX, Miao ZY, Chen LL, Zhao W et al (2010) Amperometric biosensors based on gold nanoparticles-decorated multiwalled carbon nanotubes-poly(diallyldimethylammonium chloride) biocomposite for the determination of choline. Sensors Actuators B 147:593–598

    Article  Google Scholar 

  19. Zhang YW, Chang GH, Liu S, Lu WB, Tian JQ, Sun XP (2011) A new preparation of Au nanoplates and their application for glucose sensing. Biosens Bioelectron 28:344–348

    Article  CAS  Google Scholar 

  20. Lin JH, He CY, Zhao Y, Zhang SS (2009) One-step synthesis of silver nanoparticles/carbon nanotubes/chitosan film and its application in glucose biosensor. Sensors Actuators B 137:768–773

    Article  Google Scholar 

  21. Qin XY, Lu WB, Luo YL, Chang GH, Asiri AM et al (2012) Synthesis of Ag nanoparticle-decorated 2,4,6-tris(2-pyridyl)-1,3,5-triazine nanobelts and their application for H2O2 and glucose detection. Analyst 137:939–943

    Article  CAS  Google Scholar 

  22. Zhang YW, Liu S, Wang L, Qin XY, Tian JQ, Lu WB et al (2012) One-pot green synthesis of Ag nanoparticles-graphene nanocomposites and their applications in SERS, H2O2, and glucose sensing. RSC Adv 2:538–545

    Article  CAS  Google Scholar 

  23. Lu WB, Luo YL, Chang GH, Sun XP (2011) Synthesis of functional SiO2-coated graphene oxide nanosheets decorated with Ag nanoparticles for H2O2 and glucose detection. Biosens Bioelectron 26:4791–4797

    Article  CAS  Google Scholar 

  24. Gutés A, Carraro C, Maboudian R (2012) Single-layer CVD-grown graphene decorated with metal nanoparticles as a promising biosensing platform. Biosens Bioelectron 33:56–59

    Article  Google Scholar 

  25. Baby TT, Jyothirmayee Aravind SS, Arochiadoss T, Rakhi RB, Ramaprabhu S (2010) Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor. Sensors Actuators B 145:71–77

    Article  Google Scholar 

  26. Wu H, Wang J, Kang XH, Wang CM, Wang DH, Liu J, Aksay IA, Lin YH (2009) Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. Talanta 80:403–406

    Article  CAS  Google Scholar 

  27. Deng SY, Jian GQ, Lei JP, Hu Z, Ju HX (2009) A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes. Biosens Bioelectron 25:373–377

    Article  CAS  Google Scholar 

  28. Liu SQ, Ju HX (2003) Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens Bioelectron 19:177–183

    Article  CAS  Google Scholar 

  29. Liu S, Tian JQ, Wang L, Luo YL, Lu WB, Sun XP (2011) Self-assembled graphene platelet-glucose oxidase nanostructures for glucose biosensing. Biosens Bioelectron 26:4491–4496

    Article  CAS  Google Scholar 

  30. Cai CX, Chen J (2004) Direct electron transfer of glucose oxidase promoted by carbon nanotubes. Anal Biochem 332:75–83

    Article  CAS  Google Scholar 

  31. Salimi A, Compton RG, Hallaj R (2004) Glucose biosensor prepared by glucose oxidase encapsulated sol-gel and carbon-nanotube-modified basal plane pyrolytic graphite electrode. Anal Biochem 333:49–56

    Article  CAS  Google Scholar 

  32. Liu X, Shi L, Niu W, Li H, Xu G (2008) Amperometric glucose biosensor based on single-walled carbon nanohorns. Biosens Bioelectron 23:1887–1890

    Article  CAS  Google Scholar 

  33. Zhou M, Shang L, Li B, Huang L, Dong S (2008) Highly ordered mesoporous carbons as electrode material for the construction of electrochemical dehydrogenase- and oxidase-based biosensors. Biosens Bioelectron 24:442–447

    Article  CAS  Google Scholar 

  34. Chen SH, Yuan R, Chai YQ, Zhang LY, Wang N, Li XL (2007) Amperometric thirdgeneration hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles. Biosens Bioelectron 22:1268–1274

    Article  CAS  Google Scholar 

  35. Yang J, Yang T, Feng YY, Jiao K (2007) A DNA electrochemical sensor based on nanogold-modified poly-2,6-pyridinedicarboxylic acid film and detection of PAT gene fragment. Anal Biochem 365:24–30

    Article  CAS  Google Scholar 

  36. Peng H, Soeller C, Cannell MB, Bowmaker GA, Cooney RP, Sejdic JT (2006) Electrochemical detection of DNA hybridization amplified by nanoparticles. Biosens Bioelectron 21:1727–1736

    Article  CAS  Google Scholar 

  37. Reisberg S, Dang LA, Nguyen QA, Piro B, Noel V, Nielsen PE et al (2008) Label-free DNA electrochemical sensor based on a PNA-functionalized conductive polymer. Talanta 76:206–210

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Science Foundation of China (50876058), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, and the Program for New Century Excellent Talents in University (NCET-10-883).

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Correspondence to Huaqing Xie.

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Chen, L., Xie, H. & Li, J. Electrochemical glucose biosensor based on silver nanoparticles/multiwalled carbon nanotubes modified electrode. J Solid State Electrochem 16, 3323–3329 (2012). https://doi.org/10.1007/s10008-012-1773-9

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  • DOI: https://doi.org/10.1007/s10008-012-1773-9

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