Abstract
We incidentally found the expression of D2-40 in Cajal interstitial cells for the immunohistochemical valuation of lymphatic invasion by gastric and colonic cancer cells. Therefore, we suggested that D2-40 might become an available marker for gastrointestinal stromal tumors (GISTs). In this study, we performed immunohistochemical study of 21 GISTs, As a result, we confirmed a positive reaction for D2-40 in sixteen GISTs. Among sixteen tumors, thirteen tumors were focally positive and remaining three tumors were diffusely positive. We could find no relationship between positive findings for D2-40 and size or location of the tumor. Three of four tumors with intermediate risk of aggressive behavior and two of four tumors with recurrence showed a negative reaction for D2-40. In conclusion, we suggest that D2-40 may be an available marker of GISTs, but the expression of D2-40 seems to be not associated with the degree of risk of aggressive behavior.
Similar content being viewed by others
References
Hirota S, Isozaki K, Moriyama Y, Hashimoto K, Nishida T, Ishiguro S, Kawano K, Hanada M, Kurata A, Takeda M, Muhammad Tunio G, Matsuzawa Y, Kanakura Y, Shinomura Y, Kitamura Y (1998) Gain-of-function mutations in c-kit in human gastrointestinal stromal tumors. Science 279:577–580
Nishida T, Hirota S, Taniguchi M, Hashimoto K, Isozaki K, Nakamura H, Kanakura Y, Tanaka T, Takabayashi A, Matsuda H, Kitamura Y (1998) Familial gastrointestinal stromal tumours with germline mutation of the KIT gene. Nat Genet 19:323–324
Tsujimura T, Makiishi-Shimobayashi C, Lundkvist J, Lendahl U, Nakasho K, Sugihara A, Iwasaki T, Mano M, Yamada N, Yamashita K, Toyosaka A, Terada N (2001) Expression of the intermediate filament nestin in gastrointestinal stromal tumors and interstitial cell of Cajal. Am J Pathol 158:817–823
Sarlomo-Rikala M, Tsujimura T, Lendahl U, Miettinen M (2002) Patterns of nestin and other filament expression distinguish between gastrointestinal stromal tumors, leiomyomas and schwannomas. APMIS 110:499–507
Hasegawa T, Tatsuno Y, Shimoda T, Hirohashi S (2002) Gastrointestinal stromal tumor: consistent CD117 immunostaining for diagnosis, and prognostic classification based on tumor size and MIB-1 grade. Hum Pathol 33:669–676
Ftetcher CD, Berman JJ, Corless C, Gorstein F, Lasota J, Longley BJ, Miettinen M, O’Leary TJ, Remotti H, Rubin BP, Shmookler B, Sobin LH, Weiss SW (2002) Diagnosis of gastrointestinal stromal tumors: A consensus approach. Hum Pathol 33:459–465
Rosai J (2003) GIST: an update. Int J Surg Pathol 11:117–186
Kahn HJ, Bailey D, Marks A (2002) Monoclonal antibody D2-40, a new marker of lymphatic endothelium, react with Kaposi sarcoma and subset of angiosarcomas. Mod Pathol 15:434–440
Kahn HJ, Marks A (2002) A new monoclonal antibody, D2-40, for detection of lymphatic invasion in pulmonary tumors. Lab Invest 82:1255–1257
Chu AY, Litzky LA, Pasha TL, Acs G, Zhang PJ (2005) Utility of D2-40, a novel mesothelial marker, in the diagnosis of malignant mesothelioma. Mod Pathol 18:105–110
Ordonez NG (2005) D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma. Hum Pathol 36:372–380
Ordonez NG (2005) Immunohistochemical diagnosis of epithelioid mesothelioma: an update. Arch Pathol Lab Med 129:1407–1414
Ordonez NG (2006) The diagnostic utility of immunohistochemistry and electron microscopy in distinguishing between peritoneal mesotheliomas and serous carcinomas: a comparative study. Mod Pathol 19:34–48
Muller AM, Franke FE, Muller KM (2006) D2-40: a reliable marker in the diagnosis of pleural mesothelioma. Pathobiology 73:50–54
Attanoos RL, Gibbs AR (1997) Pathology of malignant mesothelioma. Histopathology 30:413–418
Hamamatsu A, Arai T, Iwamoto M, Kato T, Sawabe M (2005) Adenomatoid tumor of the adrenal gland: case report with immunohistochemical study. Pathol Int 55:665–669
Kuroda N, Toi M, Hiroi M, Lee GH (2007) Diagnostic pitfall of D2-40 in adenomatoid tumor. Histopathology 51:719–721
Hansen T, Katenkamp K, Katenkamp D (2006) D2-40 staining in sinonasal-type hemangiopericytoma-further evidence of distinction from conventional hemangiopericytoma and solitary fibrous tumor. Virchows Arch 448:459–462
Naito Y, Ishii G, Kawai O, Hasebe T, Nishikawa Y, Nagai K, Ochiai A (2007) D2-40-positive solitary fibrous tumors of the pleura: diagnostic pitfall of biopsy specimen. Pathol Int 57:618–621
Yu H, Gibson JA, Pinkus GS, Hornick JL (2007) Podoplanin (D2-40) is a novel marker for follicular dendritic cell tumors. Am J Clin Pathol 128:776–782
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kuroda, N., Tanida, N., Oonishi, K. et al. Significance of D2-40 expression in the diagnosis of gastrointestinal stromal tumor. Med Mol Morphol 41, 109–112 (2008). https://doi.org/10.1007/s00795-007-0393-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00795-007-0393-4