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Viscosities of mixtures of hyaluronic acids with different molecular weights and their effects on enzymatic activities of lysozyme and peroxidase

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Abstract

Objectives

To investigate the viscosity values of mixtures of hyaluronic acids with different molecular weights and the effects of these mixtures on the enzymatic activities of lysozyme and peroxidase.

Materials and methods

Mixtures of high molecular weight (1 or 2 MDa) and low molecular weight (10 or 100 kDa) hyaluronic acids at different concentrations were used for viscosity measurements. Hyaluronic acid mixtures showing viscosity values similar to those of human whole saliva were used for enzyme experiments in solution and on hydroxyapatite surface. Hen egg-white lysozyme, bovine lactoperoxidase, and human whole saliva were used as enzyme sources. Lysozyme activity was measured by hydrolysis of fluorescein-labeled Micrococcus lysodeikticus. Peroxidase activity was measured by oxidation of fluorogenic 2′,7′-dichlorofluorescein to fluorescing 2′,7′-dichlorofluorescein.

Results

The mixtures of 1 MDa (0.5 mg/mL) or 2 MDa (0.2 mg/mL) hyaluronic acid with 10 kDa (2.0 mg/mL) or 100 kDa (0.1 mg/mL) hyaluronic acid had viscosity values similar to those of human whole saliva at shear rates, reflecting normal oral functions. Compared with single molecular weight hyaluronic acids, these mixtures showed viscosity values more similar to those of human whole saliva. The mixtures inhibited lysozyme and peroxidase activities on the hydroxyapatite surfaces; however, the degree of inhibition did not differ from that of hyaluronic acid of 1 or 2 MDa only.

Conclusions

Compared with single molecular weight hyaluronic acids, hyaluronic acid mixtures showed viscosity values more similar to those of human whole saliva, without additional inhibitory effects on lysozyme and peroxidase activities.

Clinical relevance

Hyaluronic acid mixtures offer distinct advantages for the development of saliva substitutes.

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Acknowledgments

We are grateful to Dr. Ji-Young Ko and Dr. Jihoon Kim for the data input and figure drawing.

Funding

This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (no. 2016R1A2B4007286).

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Authors and Affiliations

  1. Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 03080, South Korea

    Hong-Seop Kho, Ji-Youn Chang & Yoon-Young Kim

  2. Institute on Aging, Seoul National University, Seoul, South Korea

    Hong-Seop Kho

Authors
  1. Hong-Seop Kho
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  2. Ji-Youn Chang
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  3. Yoon-Young Kim
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Correspondence to Hong-Seop Kho.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee (the Institutional Review Board of the Seoul National University Dental Hospital, #CRI18004) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all participants included in the study.

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Kho, HS., Chang, JY. & Kim, YY. Viscosities of mixtures of hyaluronic acids with different molecular weights and their effects on enzymatic activities of lysozyme and peroxidase. Clin Oral Invest 24, 3961–3970 (2020). https://doi.org/10.1007/s00784-020-03262-z

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  • DOI: https://doi.org/10.1007/s00784-020-03262-z

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