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Do edible oils reduce bacterial colonization of enamel in situ?

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Abstract

Objective

Edible oils are an empiric approach for the prevention of oral diseases. The present in situ study investigated the effect of edible oils on initial bacterial colonization of enamel surfaces.

Methods and materials

Initial biofilm formation was performed on enamel specimens mounted on maxillary splints and carried by eight subjects. After 1 min of pellicle formation, rinses with safflower oil, olive oil and linseed oil were performed for 10 min. Application of chlorhexidine for 1 min served as positive control. Afterwards, the slabs were carried for 8 h overnight. Samples carried for 8 h without any rinse served as negative controls. The amount of adherent bacteria was determined by DAPI staining (4′,6-diamidino-2-phenylindole) and live–dead staining (BacLight). Additionally, determination of colony forming units was performed after desorption of the bacteria. TEM evaluation was carried out after application of the rinses.

Results

The number of adherent bacteria on control samples was 6.1 ± 8.1 × 105/cm2 after 8 h (DAPI). Fluorescence microscopic data from DAPI staining and live–dead staining as well as from the determination of CFU revealed no significant effects of rinsing with oils on the amount of adherent bacteria compared to the non-rinsed control samples. However, with chlorhexidine a significant reduction in the number of bacteria by more than 85 % was achieved (DAPI, chlorhexidine: 8.2 ± 17.1 × 104/cm2). The ratio of viable to dead bacteria was almost equal (1:1) irrespective of the rinse adopted as recorded with BacLight. TEM indicated accumulation of oil micelles at the pellicle's surface and modification of its ultrastructure.

Conclusion

Rinses with edible oils have no significant impact on the initial pattern and amount of bacterial colonization on enamel over 8 h.

Clinical relevance

Rinses with edible oils cannot be recommended for efficient reduction of oral biofilm formation.

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References

  1. Longbottom, C, Ekstrand, K, Zero, D, Kambara, M (2009) Novel preventive treatment options. Monogr Oral Sci 155–163

  2. Deng DM, ten Cate JM, Crielaard W (2007) The adaptive response of Streptococcus mutans towards oral care products: involvement of the ClpP serine protease. Eur J Oral Sci 115:363–70

    Article  PubMed  Google Scholar 

  3. Vissink A, Mitchell JB, Baum BJ, Limesand KH, Jensen SB, Fox PC, Elting LS, Langendijk JA, Coppes RP, Reyland ME (2010) Clinical management of salivary gland hypofunction and xerostomia in head-and-neck cancer patients: successes and barriers. Int J Radiat Oncol Biol Phys 78:983–91

    Article  PubMed  Google Scholar 

  4. Ship JA, McCutcheon JA, Spivakovsky S, Kerr AR (2007) Safety and effectiveness of topical dry mouth products containing olive oil, betaine, and xylitol in reducing xerostomia for polypharmacy-induced dry mouth. J Oral Rehabil 34:724–32

    Article  PubMed  Google Scholar 

  5. Asokan S, Emmadi P, Chamundeswari R (2009) Effect of oil pulling on plaque induced gingivitis: a randomized, controlled, triple-blind study. Indian J Dent Res 20:47–51

    Article  PubMed  Google Scholar 

  6. Asokan S, Rathan J, Muthu MS, Rathna PV, Emmadi P (2008) Effect of oil pulling on Streptococcus mutans count in plaque and saliva using Dentocult SM Strip mutans test: a randomized, controlled, triple-blind study. J Indian Soc Pedod Prev Dent 26:12–7

    Article  PubMed  Google Scholar 

  7. Ravikumar N, Suhas S (2009) Not recommended Br Dent J 207:518

    Article  Google Scholar 

  8. Aguiar AA, Saliba NA (2004) Toothbrushing with vegetable oil: a clinical and laboratorial analysis. Braz Oral Res 18:168–73

    Article  PubMed  Google Scholar 

  9. Pretty IA, Gallagher MJ, Martin MV, Edgar WM, Higham SM (2003) A study to assess the effects of a new detergent-free, olive oil formulation dentifrice in vitro and in vivo. J Dent 31:327–32

    Article  PubMed  Google Scholar 

  10. Kozlovsky A, Goldberg S, Natour I, Rogatky-Gat A, Gelernter I, Rosenberg M (1996) Efficacy of a 2-phase oil: water mouthrinse in controlling oral malodor, gingivitis, and plaque. J Periodontol 67:577–82

    Article  PubMed  Google Scholar 

  11. Marsh P, Martin M (1999) Oral microbiology, 4th edn. Wright, Oxford

    Google Scholar 

  12. Hannig C, Hannig M, Rehmer O, Braun G, Hellwig E, Al-Ahmad A (2007) Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ. Arch Oral Biol 52:1048–56

    Article  PubMed  Google Scholar 

  13. Slomiany BL, Murty VL, Mandel ID, Sengupta S, Slomiany A (1990) Effect of lipids on the lactic acid retardation capacity of tooth enamel and cementum pellicles formed in vitro from saliva of caries-resistant and caries-susceptible human adults. Arch Oral Biol 35:175–80

    Article  PubMed  Google Scholar 

  14. Slomiany BL, Murty VL, Zdebska E, Slomiany A, Gwozdzinski K, Mandel ID (1986) Tooth surface-pellicle lipids and their role in the protection of dental enamel against lactic-acid diffusion in man. Arch Oral Biol 31:187–91

    Article  PubMed  Google Scholar 

  15. Wiegand A, Gutsche M, Attin T (2007) Effect of olive oil and an olive-oil-containing fluoridated mouthrinse on enamel and dentin erosion in vitro. Acta Odontol Scand 65:357–61

    Article  PubMed  Google Scholar 

  16. Olsson J, van der Heijde Y, Holmberg K (1992) Plaque formation in vivo and bacterial attachment in vitro on permanently hydrophobic and hydrophilic surfaces. Caries Res 26:428–33

    Article  PubMed  Google Scholar 

  17. Doyle R, Rosenberg M (eds) (1990) Microbilal cell surface hydrophobicity. American Society for Microbiology, Washigton, DC

    Google Scholar 

  18. Rosenberg M, Judes H, Weiss E (1983) Cell surface hydrophobicity of dental plaque microorganisms in situ. Infect Immun 42:831–4

    PubMed  Google Scholar 

  19. Carluccio MA, Siculella L, Ancora MA, Massaro M, Scoditti E, Storelli C, Visioli F, Distante A, De Caterina R (2003) Olive oil and red wine antioxidant polyphenols inhibit endothelial activation: antiatherogenic properties of Mediterranean diet phytochemicals. Arterioscler Thromb Vasc Biol 23:622–9

    Article  PubMed  Google Scholar 

  20. Carluccio MA, Massaro M, Scoditti E, De Caterina R (2007) Vasculoprotective potential of olive oil components. Mol Nutr Food Res 51:1225–34

    Article  PubMed  Google Scholar 

  21. Prasad K (2005) Hypocholesterolemic and antiatherosclerotic effect of flax lignan complex isolated from flaxseed. Atherosclerosis 179:269–75

    Article  PubMed  Google Scholar 

  22. Prasad K (2009) Flaxseed and cardiovascular health. J Cardiovasc Pharmacol 54:369–77

    Article  PubMed  Google Scholar 

  23. Carlen A, Borjesson AC, Nikdel K, Olsson J (1998) Composition of pellicles formed in vivo on tooth surfaces in different parts of the dentition, and in vitro on hydroxyapatite. Caries Res 32:447–55

    Article  PubMed  Google Scholar 

  24. Al-Ahmad A, Wunder A, Auschill TM, Follo M, Braun G, Hellwig E, Arweiler NB (2007) The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization. J Med Microbiol 56:681–7

    Article  PubMed  Google Scholar 

  25. Hannig C, Sorg J, Spitzmuller B, Hannig M, Al-Ahmad A (2009) Polyphenolic beverages reduce initial bacterial adherence to enamel in situ. J Dent 37:560–566

    Article  PubMed  Google Scholar 

  26. Schwartz T, Hoffmann S, Obst U (2003) Formation of natural biofilms during chlorine dioxide and u.v. disinfection in a public drinking water distribution system. J Appl Microbiol 95:591–601

    Article  PubMed  Google Scholar 

  27. Hannig C, Follo M, Hellwig E, Al-Ahmad A (2010) Visualization of adherent micro-organisms using different techniques. J Med Microbiol 59:1–7

    Article  PubMed  Google Scholar 

  28. Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–69

    PubMed  Google Scholar 

  29. Al-Ahmad A, Wiedmann-Al-Ahmad M, Faust J, Bachle M, Follo M, Wolkewitz M, Hannig C, Hellwig E, Carvalho C, Kohal R (2010) Biofilm formation and composition on different implant materials in vivo. J Biomed Mater Res B Appl Biomater 95:101–9

    PubMed  Google Scholar 

  30. Jung DJ, Al-Ahmad A, Follo M, Spitzmuller B, Hoth-Hannig W, Hannig M, Hannig C (2010) Visualization of initial bacterial colonization on dentine and enamel in situ. J Microbiol Methods 81:166–74

    Article  PubMed  Google Scholar 

  31. Al-Ahmad A, Roth D, Wolkewitz M, Wiedmann-Al-Ahmad M, Follo M, Ratka-Kruger P, Deimling D, Hellwig E, Hannig C (2010) Change in diet and oral hygiene over an 8-week period: effects on oral health and oral biofilm. Clin Oral Investig 14:391–6

    Article  PubMed  Google Scholar 

  32. Al-Ahmad A, Follo M, Selzer AC, Hellwig E, Hannig M, Hannig C (2009) Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. J Med Microbiol 58:1359–66

    Article  PubMed  Google Scholar 

  33. Hannig C, Sorg J, Spitzmuller B, Hannig M, Al-Ahmad A (2009) Polyphenolic beverages reduce initial bacterial adherence to enamel in situ. J Dent 37:560–6

    Article  PubMed  Google Scholar 

  34. Hannig C, Spies B, Spitzmuller B, Hannig M (2010) Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle. Arch Oral Biol 55:1–6

    Article  PubMed  Google Scholar 

  35. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE (2005) Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 43:5721–32

    Article  PubMed  Google Scholar 

  36. Rosenberg M, Bar-Ness Greenstein R, Barki M, Goldberg S (1996) Hydrophobic interactions as a basis for interfering with microbial adhesion. Adv Exp Med Biol 408:241–8

    Article  PubMed  Google Scholar 

  37. Schultz MP, Bendick JA, Holm ER, Hertel WM (2011) Economic impact of biofouling on a naval surface ship. Biofouling 27:87–98

    Article  PubMed  Google Scholar 

  38. Stein J, Truby K, Wood CD, Gardner M, Swain G, Kavanagh C, Kovach B, Schultz M, Wiebe D, Holm E, Montemarano J, Wendt D, Smith C, Meyer A (2003) Silicone foul release coatings: effect of the interaction of oil and coating functionalities on the magnitude of macrofouling attachment strengths. Biofouling 19(Suppl):71–82

    Article  PubMed  Google Scholar 

  39. Knörr W., DP, Grützmacher R., Höfer R. (1995) Development of new fields of application for linseed oil. European Journal of Lipid Science and Technology. 97:5

  40. Goldberg S, Doyle RJ, Rosenberg M (1990) Mechanism of enhancement of microbial cell hydrophobicity by cationic polymers. J Bacteriol 172:5650–4

    PubMed  Google Scholar 

  41. Kabara JJ, Vrable R (1977) Antimicrobial lipids: natural and synthetic fatty acids and monoglycerides. Lipids 12:753–9

    Article  PubMed  Google Scholar 

  42. Kabara JJ, Swieczkowski DM, Conley AJ, Truant JP (1972) Fatty acids and derivatives as antimicrobial agents. Antimicrob Agents Chemother 2:23–8

    Article  PubMed  Google Scholar 

  43. Gibbons RJ, Etherden I (1983) Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. Infect Immun 41:1190–6

    PubMed  Google Scholar 

  44. Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–53

    Article  PubMed  Google Scholar 

  45. Strevett KA, Chen G (2003) Microbial surface thermodynamics and applications. Res Microbiol 154:329–35

    Article  PubMed  Google Scholar 

  46. Doyle RJ, Rosenberg M (1995) Measurement of microbial adhesion to hydrophobic substrata. Methods Enzymol 253:542–50

    Article  PubMed  Google Scholar 

  47. Hannig C, Hannig M (2009) The oral cavity—a key system to understand substratum-dependent bioadhesion on solid surfaces in man. Clin Oral Investig 13:123–139

    Article  PubMed  Google Scholar 

  48. Bowen WH, Koo H (2011) Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res 45:69–86

    Article  PubMed  Google Scholar 

  49. Scheie AA, Kjeilen JC (1987) Effects of chlorhexidine, NaF and SnF2 on glucan formation by salivary and culture supernatant GTF adsorbed to hydroxyapatite. Scand J Dent Res 95:532–5

    PubMed  Google Scholar 

  50. Medina E, Romero C, Brenes M, De Castro A (2007) Antimicrobial activity of olive oil, vinegar, and various beverages against foodborne pathogens. J Food Prot 70:1194–9

    PubMed  Google Scholar 

  51. Romero C, Medina E, Vargas J, Brenes M, De Castro A (2007) In vitro activity of olive oil polyphenols against Helicobacter pylori. J Agric Food Chem 55:680–6

    Article  PubMed  Google Scholar 

  52. Perez-Jimenez F, Alvarez de Cienfuegos G, Badimon L, Barja G, Battino M, Blanco A, Bonanome A, Colomer R, Corella-Piquer D, Covas I, Chamorro-Quiros J, Escrich E, Gaforio JJ, Garcia Luna PP, Hidalgo L, Kafatos A, Kris-Etherton PM, Lairon D, Lamuela-Raventos R, Lopez-Miranda J, Lopez-Segura F, Martinez-Gonzalez MA, Mata P, Mataix J, Ordovas J, Osada J, Pacheco-Reyes R, Perucho M, Pineda-Priego M, Quiles JL, Ramirez-Tortosa MC, Ruiz-Gutierrez V, Sanchez-Rovira P, Solfrizzi V, Soriguer-Escofet F, de la Torre-Fornell R, Trichopoulos A, Villalba-Montoro JM, Villar-Ortiz JR, Visioli F (2005) International conference on the healthy effect of virgin olive oil. Eur J Clin Invest 35:421–4

    Article  PubMed  Google Scholar 

  53. Cicerale S, Lucas L, Keast R (2010) Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci 11:458–79

    Article  PubMed  Google Scholar 

  54. Cho SH, Lee HR, Kim TH, Choi SW, Lee WJ, Choi Y (2004) Effects of defatted safflower seed extract and phenolic compounds in diet on plasma and liver lipid in ovariectomized rats fed high-cholesterol diets. J Nutr Sci Vitaminol (Tokyo) 50:32–7

    Article  Google Scholar 

  55. Haffajee AD, Roberts C, Murray L, Veiga N, Martin L, Teles RP, Letteri M, Socransky SS (2009) Effect of herbal, essential oil, and chlorhexidine mouthrinses on the composition of the subgingival microbiota and clinical periodontal parameters. J Clin Dent 20:211–7

    PubMed  Google Scholar 

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Acknowledgments

The study was supported by a grant from the DFG (Deutsche Forschungsgemeinschaft, # HA 5192/2-1; # KU1271/6‐1). The authors would like to thank W. Hoth-Hannig, B. Spitzmüller, S. Basche and S. Pötschke for the excellent support in the laboratory.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany

    Christian Hannig, Jasmin Kirsch & Anna Kensche

  2. Department of Operative Dentistry and Periodontology University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany

    Ali Al-Ahmad

  3. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, 66421, Homburg/Saar, Germany

    Matthias Hannig

  4. Material Resources, Institute for Environmental Chemistry, Leuphana University Lüneburg, Scharnhorststraße 1/C13, 21335, Lüneburg, Germany

    Klaus Kümmerer

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  1. Christian Hannig
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Correspondence to Christian Hannig.

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Hannig, C., Kirsch, J., Al-Ahmad, A. et al. Do edible oils reduce bacterial colonization of enamel in situ?. Clin Oral Invest 17, 649–658 (2013). https://doi.org/10.1007/s00784-012-0734-0

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  • DOI: https://doi.org/10.1007/s00784-012-0734-0

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