Abstract
The purpose of this study was to compare the relative amount of low molecular weight salivary proteins in patients with head and neck tumours treated with radiotherapy and healthy subjects. Reverse-phase high-pressure liquid chromatography was used for protein separation. Nine protein fractions (including acidic and basic proline-rich proteins (PRPs), cystatins, histatins and statherin) were identified in saliva from irradiated patients as well as healthy subjects. However, compared with non-irradiated healthy subjects, the fraction of acidic PRPs was significantly reduced in irradiated patients. These data indicate an alteration of the relative amount of low molecular weight salivary proteins in irradiated patients besides the reduction of salivary flow.
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Notes
Supply from FG Oppenheim (Boston, MA, USA).
PRPs and statherin from N Stromberg (Umea, Sweden) were acknowledged.
α-Amylase from Sigma was used.
References
Almstahl A, Wikström M, Groenink J (2001) Lactoferrin, amylase and mucin MUC5B and their relation to the oral microflora in hyposalivation of different origins. Oral Microbiol Immunol 16:345–352
Anderson MW, Izutzu KT, Rice JV (1981) Parotid gland pathophysiology after mixed gamma and neutron irradation of cancer patients. Oral Surg Oral Med Oral Pathol 52:495–500
Barker WC, Garavelli JS, Huang H, McGarvey PB, Orcutt BC, Srinivasarao GY, Xiao C, Yeh LS, Ledley RS, Janda JF, Pfeiffer F, Mewes HW, Tsugita A, Wu C (2000) The protein information resource (PIR). Nucleic Acids Res 28(1):41–44
Ben-Aryeth H, Gutman D, Szargel R, Laufer D (1975) Effects of irradiation on saliva in cancer patients. Int J Oral Maxillofac Surg 4:205–210
Brown LR, Dreizen S, Rider LJ, Johnston DA (1976) The effect of radiation induced xerostomia on saliva and serum lysozyme and immunoglobulin levels. Oral Surg Oral Med Oral Pathol 41:83–92
Christensen ME, Hansen HS, Poulsen SS, Bretlau P, Nexø E (1996) Immunohisto-chemical and quantitative changes in salivary EGF, amylase and haptocorrin following radiotherapy for oral cancer. Acta Otolaryngol (Stockh) 116:137–143
Cowman RA, Baron SS, Glassman AR, Davis ME, Strosbger AM (1983) Changes in protein composition of saliva from radiation-induced xerostomia patients and its effect on growth of oral streptococci. J Dent Res 62:336–340
Dodds MW, Johnson DA, Mobley CC, Hattaway KM (1997) Parotid saliva protein profiles in caries-free and caries-active adults. Oral Surg Oral Med Oral Pathol Oral Radiol Endo 83:244–251
Edgar WM, Bowen WH, Cole MF (1982) Protein components in saliva and plaque fluid from irradiated primates. J Oral Pathol 11:252–259
Eichhorn W, Gehrke G, Eichhorst U, Schwenzer N, Linstedt-Hilden M, Greschniok A, Kaiserling E (1990) Strahleninduzierte Sialadentis und Mundschleimhautveränderungen. Z Stomatol 87:511–516
Epstein JB, Chin EA, Jacobson JJ, Rishirajb NL (1998) The relationship among fluoride, cariogenic oral flora, and salivary flow rate during radiation therapy. Oral Surg Oral Med Oral Pathol 86:286–292
Frank RM, Herdly J, Philippe E (1965) Acquired dental defects and salivary gland lesions after irradiation for carcinoma. J Am Dent Assoc 70:868–883
Funegard U, Franzen L, Ericson T, Henriksson R (1994) Parotid saliva composition during and after irradiation of head and neck cancer. Eur J Cancer B Oral Oncol 30:230–233
Kehrer U, Fischer T, Kneist S, Stößer L (1999) Reversed phase liquid chromatography (RPLC) of human salivary proteins. Caries Res 33:309–310
Liu RP, Fleming T, Toth BB, Keene HJ (1990) Salivary flow rates in patients with head and neck cancer 0.5 to 25 years after radiotherapy. Oral Surg Oral Med Oral Pathol 70:724–729
Makkonen TA, Tenovuo J, Vilja P, Heimdahl A, Tampere T (1986) Changes in the protein composition of whole saliva during radiotherapy in patients with oral or pharyngeal cancer. Oral Surg Oral Med Oral Pathol 62:270–275
Moosman KL, Shatzman AR, Chencharick J (1981) Effects of radiotherapy on human parotid saliva. Radiat Res 88:403–412
Moosman KL, Shatzman AR, Chencharick J (1982) Long term effects of radiotherapy on taste and salivary function in man. Int J Radiat Oncol Biol Phys 8:991–997
Nederfos T (2000) Xerostomia and hyposalivation. Adv Dent Res 14:48–56
Offner GD, Troxler RF (2000) Heterogeneity of high-molecular-weight human salivary mucins. Adv Dent Res 14:69–75
Pykönen H, Malmstrom M, Oikarinen VJ, Salmo M, Vehkalahti M (1986) Late effects of radiation treatment of tongue and floor-of-mouth cancer on the dentition, saliva secretion, mucous membranes and the lower jaw. Int J Oral Maxillofac Surg 15:401–409
Schubert MM, Izutsu KT (1987) Iatrogenic causes of salivary gland dysfunction. J Dent Res 66:680–688
Shannon IL, Starcke EN, Wescott WB (1997) Effect of radiotherapy on whole saliva flow. J Dent Res 56:693
Stenudd C, Nordlund A, Ryberg M, Johansson I, Kallestal C, Stromberg N (2001) The association of bacterial adhesion with dental caries. J Dent Res 80:2005–2010
Walz A, Wattenberg A, Meyer HE, Schmalz G, Blüggel M, Ruhl S (2006) Proteome analysis of glandular parotid and submandibular–sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis. Proteomics 6(8) (in press)
Valdez IH, Atkinson JC, Ship JA, Fox PC (1993) Major salivary gland function in patients with radiation-induced xerostomia: flow rates and sialochemistry. Int J Radiat Oncol Biol Phys 25:41–47
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This study was supported by a grant of Deutsche Forschungsgemeinschaft (STO 265/4-1).
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Hannig, M., Dounis, E., Henning, T. et al. Does irradiation affect the protein composition of saliva?. Clin Oral Invest 10, 61–65 (2006). https://doi.org/10.1007/s00784-005-0026-z
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DOI: https://doi.org/10.1007/s00784-005-0026-z