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Visualization of hydrogen polysulfides in living cells and in vivo via a near-infrared fluorescent probe

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Abstract

Hydrogen polysulfides (H2Sn, n > 1), as the oxidized forms of H2S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H2Sn in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1, with “turn-on” response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H2Sn. The probe was successfully applied to the visualizing of endogenous H2Sn in living cells. Moreover, it can be used for near-infrared in vivo imaging of H2Sn in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H2Sn in living systems.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21971115, 21501085) and Key University Science Research Project of Jiangsu Province (17KJA150004).

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Authors and Affiliations

  1. College of Science, Nanjing Forestry University, Nanjing, 210037, China

    Xiaoqing Wang, Qian Sun & Li Xu

  2. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, China

    Liming Zhao & Shuwen Gong

  3. Institute of Material Physics and Chemistry, Nanjing Forestry University, Nanjing, 210037, China

    Li Xu

Authors
  1. Xiaoqing Wang
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  2. Qian Sun
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  3. Liming Zhao
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  4. Shuwen Gong
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  5. Li Xu
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Correspondence to Xiaoqing Wang or Li Xu.

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Wang, X., Sun, Q., Zhao, L. et al. Visualization of hydrogen polysulfides in living cells and in vivo via a near-infrared fluorescent probe. J Biol Inorg Chem 24, 1077–1085 (2019). https://doi.org/10.1007/s00775-019-01718-6

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  • DOI: https://doi.org/10.1007/s00775-019-01718-6

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